03316nam 22007453a 450 991034685410332120250203235426.09783039212286303921228110.3390/books978-3-03921-228-6(CKB)4920000000095126(oapen)https://directory.doabooks.org/handle/20.500.12854/40821(ScCtBLL)e22c1d16-7a66-45b0-aaaf-60601e75c5fd(OCoLC)1163845492(EXLCZ)99492000000009512620250203i20192019 uu engurmn|---annantxtrdacontentcrdamediacrrdacarrierAnalysis of Peptides and Proteins by Electrophoretic TechniquesAngela R. Piergiovanni, José Manuel Herrero-MartínezBasel, Switzerland :MDPI,2019.1 electronic resource (110 p.)9783039212279 3039212273 The characterization of peptides and proteins is central to understanding their function and expression in biological matrices. Moreover, these macromolecules are important biomarkers of many human diseases. In recent years, the performance of separation techniques based on electromigration have significantly increased. The development of microdevices has reduced sample consumption and waste production while high-sensitivity detectors, such as mass spectrometry (MS) or laser-induced fluorescence (LIF), have significantly improved with regards to separation efficiency and detection limits. All of these advancements have led to appreciably enlarged fields of application. Nowadays, a multitude of studies using separation techniques based on electromigration to study proteins and peptides from numerous real matrices are available in the literature. This Special Issue covers the most recent knowledge and advances in the study of peptides and proteins using several electrophoresis techniques, as well as the characterization of relevant proteins and peptides in application areas such as clinical studies, functional foods, and toxicology.seed phosphoproteomicsLC-ESI-MSpuritytransferrinCE-LIFenzyme assaythioglucosidasecapillary electrophoresisvenom compositionimmunoassayfluorescenceseed proteomicsSEC-HPLCdesulfo-sinigrinmetalloproteinsseed quality traitsseed glycoproteomicsproteomicssulfataseon-gel detectionnon-covalent bindingNaja asheimyrosinaseSDS-PAGEcarbon dots2-D electrophoresisfragmentrhIL-12seed molecular breedingchip-based CE-LIF assayPiergiovanni Angela R1788593Herrero-Martínez José ManuelScCtBLLScCtBLLBOOK9910346854103321Analysis of Peptides and Proteins by Electrophoretic Techniques4323633UNINA