03061nam 2200661z- 450 991034685410332120240102210720.03-03921-228-1(CKB)4920000000095126(oapen)https://directory.doabooks.org/handle/20.500.12854/40821(EXLCZ)99492000000009512620202102d2019 |y 0engurmn|---annantxtrdacontentcrdamediacrrdacarrierAnalysis of Peptides and Proteins by Electrophoretic TechniquesMDPI - Multidisciplinary Digital Publishing Institute20191 electronic resource (110 p.)3-03921-227-3 The characterization of peptides and proteins is central to understanding their function and expression in biological matrices. Moreover, these macromolecules are important biomarkers of many human diseases. In recent years, the performance of separation techniques based on electromigration have significantly increased. The development of microdevices has reduced sample consumption and waste production while high-sensitivity detectors, such as mass spectrometry (MS) or laser-induced fluorescence (LIF), have significantly improved with regards to separation efficiency and detection limits. All of these advancements have led to appreciably enlarged fields of application. Nowadays, a multitude of studies using separation techniques based on electromigration to study proteins and peptides from numerous real matrices are available in the literature. This Special Issue covers the most recent knowledge and advances in the study of peptides and proteins using several electrophoresis techniques, as well as the characterization of relevant proteins and peptides in application areas such as clinical studies, functional foods, and toxicology.seed phosphoproteomicsLC-ESI-MSpuritytransferrinCE-LIFenzyme assaythioglucosidasecapillary electrophoresisvenom compositionimmunoassayfluorescenceseed proteomicsSEC-HPLCdesulfo-sinigrinmetalloproteinsseed quality traitsseed glycoproteomicsproteomicssulfataseon-gel detectionnon-covalent bindingNaja asheimyrosinaseSDS-PAGEcarbon dots2-D electrophoresisfragmentrhIL-12seed molecular breedingchip-based CE-LIF assayHerrero-Martínez José Manuelauth1284079R. Piergiovanni AngelaauthBOOK9910346854103321Analysis of Peptides and Proteins by Electrophoretic Techniques3019272UNINA