03749nam 22006015 450 991030055730332120200629122522.03-319-98255-910.1007/978-3-319-98255-7(CKB)4100000005679250(MiAaPQ)EBC5491451(DE-He213)978-3-319-98255-7(PPN)229915531(EXLCZ)99410000000567925020180809d2018 u| 0engurcnu||||||||txtrdacontentcrdamediacrrdacarrierSpectrally Resolved Detector Arrays for Multiplexed Biomedical Fluorescence Imaging /by Anna Siri Luthman1st ed. 2018.Cham :Springer International Publishing :Imprint: Springer,2018.1 online resource (153 pages)Springer Theses, Recognizing Outstanding Ph.D. Research,2190-50533-319-98254-0 Summary and Rationale -- Spectral Imaging Systems and Sensor Characterisations -- Wide-field fHSI with a Linescan SRDA -- A Multispectral Endoscope Based on SRDAs -- Conclusions and Outlook.This book describes the design, development, characterisation and application of two novel fluorescence imaging instruments based on spectrally resolved detector arrays (SRDAs). The simplest SRDA is the standard colour camera, which integrates a Bayer filter array of red, green and blue colour filters to replicate the colour sensing capability of the human eye. The SRDAs used in this book contain many more colours, ranging from 16 to over 100 colour channels. Using these compact, robust and low-cost detectors for biomedical applications opens new avenues of exploration that were not possible before, in particular, the use of spectral imaging in endoscopy. The work presented shows for the first time that not only can this new type of camera be used for fluorescence imaging, but also that it is able to resolve signals from up to 7 different dyes – a level of multiplexing not previously achieved in tissue with such compact and robust equipment. Furthermore, it reports the application of a bimodal endoscope performing both reflectance and fluorescence imaging using these cameras in an ex vivo pig oesophagus model. This is the first book to demonstrate the potential of SRDAs for fluorescence imaging in biomedicine, opening up a new multidisciplinary research direction.Springer Theses, Recognizing Outstanding Ph.D. Research,2190-5053Spectrum analysisMicroscopyBiophysicsBiophysicsPhysical measurementsMeasurementSpectroscopy and Microscopyhttps://scigraph.springernature.com/ontologies/product-market-codes/P31090Biological and Medical Physics, Biophysicshttps://scigraph.springernature.com/ontologies/product-market-codes/P27008Measurement Science and Instrumentationhttps://scigraph.springernature.com/ontologies/product-market-codes/P31040Spectrum analysis.Microscopy.Biophysics.Biophysics.Physical measurements.Measurement.Spectroscopy and Microscopy.Biological and Medical Physics, Biophysics.Measurement Science and Instrumentation.535.84Luthman Anna Siriauthttp://id.loc.gov/vocabulary/relators/aut1064360BOOK9910300557303321Spectrally Resolved Detector Arrays for Multiplexed Biomedical Fluorescence Imaging2537609UNINA