03591nam 22006495 450 991030054120332120200704094552.03-319-68997-510.1007/978-3-319-68997-5(CKB)4100000001382110(DE-He213)978-3-319-68997-5(MiAaPQ)EBC5211335(PPN)222228164(EXLCZ)99410000000138211020171230d2018 u| 0engurnn|008mamaatxtrdacontentcrdamediacrrdacarrierCellular Imaging Electron Tomography and Related Techniques /edited by Eric Hanssen1st ed. 2018.Cham :Springer International Publishing :Imprint: Springer,2018.1 online resource (XIX, 323 p. 84 illus., 59 illus. in color.) Biological and Medical Physics, Biomedical Engineering,1618-72103-319-68995-9 Includes bibliographical references at the end of each chapters and index.TEM Tomography -- STEM Tomography -- CryoTomo -- Large Volume Electron Tomography -- Block Face Tomography -- Array Tomography -- Image Alignment -- Reconstruction Algorithms -- Subtomogram Averaging -- Resolution -- Signal Optimisation -- Segmentation -- Placing Macromolecular Complexes in Context.This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging).  The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation.Biological and Medical Physics, Biomedical Engineering,1618-7210BiophysicsBiophysicsSpectrum analysisMicroscopyBiomedical engineeringBiological and Medical Physics, Biophysicshttps://scigraph.springernature.com/ontologies/product-market-codes/P27008Spectroscopy and Microscopyhttps://scigraph.springernature.com/ontologies/product-market-codes/P31090Biomedical Engineering and Bioengineeringhttps://scigraph.springernature.com/ontologies/product-market-codes/T2700XSpectroscopy/Spectrometryhttps://scigraph.springernature.com/ontologies/product-market-codes/C11020Biophysics.Biophysics.Spectrum analysis.Microscopy.Biomedical engineering.Biological and Medical Physics, Biophysics.Spectroscopy and Microscopy.Biomedical Engineering and Bioengineering.Spectroscopy/Spectrometry.616.07572Hanssen Ericedthttp://id.loc.gov/vocabulary/relators/edtMiAaPQMiAaPQMiAaPQBOOK9910300541203321Cellular Imaging2529262UNINA