02988oam 2200457 450 991029864010332120190911112726.03-642-42008-710.1007/978-3-642-42008-5(OCoLC)878127870(MiFhGG)GVRL6WUK(EXLCZ)99371000000007483320131023d2014 uy 0engurun|---uuuuatxtccrApplications of monolithic column and isotope dimethylation labeling in shotgun proteome analysis /Fangjun Wang1st ed. 2014.Heidelberg, Germany :Springer,2014.1 online resource (xiii, 94 pages) illustrations (some color)Springer Theses, Recognizing Outstanding Ph.D. Research,2190-5053"ISSN: 2190-5053."3-642-42007-9 Includes bibliographical references.Introduction -- Online sample injection and multidimensional chromatography separation by using strong cation exchange monolithic column -- Development of polymer-based hydrophobic monolithic columns and their applications in proteome analysis -- Large-scale proteome and phosphoproteome quantification by using dimethylation isotope labeling.In this thesis, the author outlines the development of new monolithic columns and isotope dimethyl labeling strategies and their applications in high-performance proteome analyses. Though different types of monolithic columns have been widely developed for chromatography and electrophoresis separation, their application in proteomics for complex peptide mixtures separation is still a challenge. The author discusses the preparation of new monolithic columns and optimization of chromatography separation capability to improve coverage and accuracy of proteome analysis. Further, the author describes a novel online multidimensional chromatography system combined with automated online isotope labeling, which significantly improves the throughput, sensitivity, and accuracy of quantitative proteomics. In addition to the development of new technologies, the author investigates the proteome and phosphoproteome expression changes of clinical hepatocellular carcinoma tissues and the hippocampi of mice with Alzheimer’s disease. The work in this thesis has led to several publications in high-profile journals in the fields of analytical chemistry and proteome research.Springer theses.ProteinsAnalysisProteomicsProteinsAnalysis.Proteomics.543.65Wang Fangjunauthttp://id.loc.gov/vocabulary/relators/aut1061947MiFhGGMiFhGGBOOK9910298640103321Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis2521559UNINA