04449nam 22007335 450 991029863390332120200702123012.03-662-47293-710.1007/978-3-662-47293-4(CKB)3710000000416750(EBL)2095480(SSID)ssj0001500940(PQKBManifestationID)11852693(PQKBTitleCode)TC0001500940(PQKBWorkID)11536264(PQKB)10000603(DE-He213)978-3-662-47293-4(MiAaPQ)EBC2095480(PPN)186027656(EXLCZ)99371000000041675020150526d2015 u| 0engur|n|---|||||txtccrMicroRNA Detection and Pathological Functions /by Xueji Zhang, Haifeng Dong, Yaping Tian1st ed. 2015.Berlin, Heidelberg :Springer Berlin Heidelberg :Imprint: Springer,2015.1 online resource (108 p.)SpringerBriefs in Molecular Science,2191-5407Description based upon print version of record.3-662-47292-9 Includes bibliographical references at the end of each chapters.Introduction -- miRNAs Biology in Pathological Processes -- Conventional MiRNA Detection Strategies -- MiRNA Electrochemical Detection -- MiRNA Optical Detection.- Other Emerging MiRNA Detection Strategies -- Intracellular and Organic miRNA in Situ Detection -- Summary and Prospects.This book summarizes microRNA (miRNA) biology in a variety of pathological processes, emphasizing the significant potential applications of miRNA in diagnostics and prognostics, as well as novel drug targets. The conventional techniques used for miRNA detection including standard PCR, Northern blotting, microarray and clone methods are addressed. Recent emerging strategies in miRNA detection and quantification with superior flexibility and adaptability, such as novel molecular biological techniques and locked nucleic acid (LNA) modified probes, as well as nanotechnology-based approaches, are also included. The book also highlights the latest advances in clinical-related miRNA detection methods in living cells, circulating blood and tissue, such as in situ hybridization (ISH) and molecular imaging techniques, which are useful to elucidate the biogenesis and biological function of miRNAs in vivo. Finally, the respective advantages and drawbacks of various detection techniques in this fast-moving field are discussed, along with the challenges and promising new directions. This book offers a valuable resource for analytical chemists, biologists and physicians involved in miRNA research. Dr. Xueji Zhang and Dr. Haifeng Dong are Professors at the School of Chemistry & Biological Engineering, University of Science & Technology Beijing (USTB), China. Dr. Yaping Tian is a Professor at the Department of Clinical Biochemistry, Chinese PLA General Hospital and Military Medical School, China.SpringerBriefs in Molecular Science,2191-5407Analytical chemistryCancer researchNucleic acidsGene therapyAnalytical Chemistryhttps://scigraph.springernature.com/ontologies/product-market-codes/C11006Cancer Researchhttps://scigraph.springernature.com/ontologies/product-market-codes/B11001Nucleic Acid Chemistryhttps://scigraph.springernature.com/ontologies/product-market-codes/L14011Gene Therapyhttps://scigraph.springernature.com/ontologies/product-market-codes/B12020Analytical chemistry.Cancer research.Nucleic acids.Gene therapy.Analytical Chemistry.Cancer Research.Nucleic Acid Chemistry.Gene Therapy.572.88Zhang Xuejiauthttp://id.loc.gov/vocabulary/relators/aut991428Dong Haifengauthttp://id.loc.gov/vocabulary/relators/autTian Yapingauthttp://id.loc.gov/vocabulary/relators/autMiAaPQMiAaPQMiAaPQBOOK9910298633903321MicroRNA Detection and Pathological Functions2268945UNINA