04699nam 22006975 450 991029857960332120251116195418.03-319-73405-910.1007/978-3-319-73405-7(CKB)4100000002892254(MiAaPQ)EBC5357938(DE-He213)978-3-319-73405-7(PPN)22554864X(EXLCZ)99410000000289225420180302d2018 u| 0engurcnu||||||||txtrdacontentcrdamediacrrdacarrierFluorescent Tools for Imaging Oxidative Stress in Biology /by Amandeep Kaur1st ed. 2018.Cham :Springer International Publishing :Imprint: Springer,2018.1 online resource (265 pages) illustrationsSpringer Theses, Recognizing Outstanding Ph.D. Research,2190-5053"Doctoral thesis accepted by the University of Sydney, Australia."3-319-73404-0 Introduction -- Flavin based redox probes -- FRET based ratiometric redox probes -- Mitochondrially-targeted ratiometric redox probes -- Nicotinamide based ratiometric probes -- In cellulo studies -- Ex vivo studies -- Non-mammalian systems -- Conclusions -- Experimental Methods. .This thesis advances the long-standing challenge of measuring oxidative stress and deciphering its underlying mechanisms, and also outlines the advantages and limitations of existing design strategies. It presents a range of approaches for the chemical synthesis of fluorescent probes that detect reversible changes in cellular oxidative stress. The ability to visualise cellular processes in real-time is crucial to understanding disease development and streamline treatment, and this can be achieved using fluorescent tools that can sense reversible disturbances in cellular environments during pathogenesis. The perturbations in cellular redox state are of particular current interest in medical research, since oxidative stress is implicated in the pathogenesis of a number of diseases. The book investigates different strategies used to achieve ratiometric fluorescence output of the reversible redox probes, which nullify concentration effects associated with intensity-based probes. It also describes suitable approaches to target these probes to specific cellular organelles, thereby enabling medical researchers to visualise sub-cellular oxidative stress levels, and addressing the typically poor uptake of chemical tools into biological studies. In total it reports on four new probes that are now being used by over twenty research groups around the globe, and two of which have been commercialised. The final chapters of this thesis demonstrate successful applications of the sensors in a variety of biological systems ranging from prokaryotes to mammalian cells and whole organisms. The results described clearly indicate the immense value of collaborative, cross-disciplinary research.Springer Theses, Recognizing Outstanding Ph.D. Research,2190-5053Analytical chemistryOxidative stressBiophysicsBiophysicsBiomedical engineeringChemistry, Physical and theoreticalAnalytical Chemistryhttps://scigraph.springernature.com/ontologies/product-market-codes/C11006Oxidative Stresshttps://scigraph.springernature.com/ontologies/product-market-codes/L16070Biological and Medical Physics, Biophysicshttps://scigraph.springernature.com/ontologies/product-market-codes/P27008Biomedical Engineering/Biotechnologyhttps://scigraph.springernature.com/ontologies/product-market-codes/B24000Physical Chemistryhttps://scigraph.springernature.com/ontologies/product-market-codes/C21001Analytical chemistry.Oxidative stress.Biophysics.Biophysics.Biomedical engineering.Chemistry, Physical and theoretical.Analytical Chemistry.Oxidative Stress.Biological and Medical Physics, Biophysics.Biomedical Engineering/Biotechnology.Physical Chemistry.616.39Amandeep Kaurauthttp://id.loc.gov/vocabulary/relators/aut1879100MiAaPQMiAaPQMiAaPQBOOK9910298579603321Fluorescent Tools for Imaging Oxidative Stress in Biology4498461UNINA