01308nam 2200337Ia 450 99638379640331620221108051900.0(CKB)1000000000596224(EEBO)2248521506(OCoLC)12847743(EXLCZ)99100000000059622419851126d1641 uy |engurbn||||a|bb|An answer to a printed paper entituled Articles exhibited in Parliament against Mr. John Sqvier, viccar of Saint Leonard Shoreditch, August 7, 1641[electronic resource][London? s.n.]1641[2], 11, [1] pReproduction of originals in Thomason Collection, British Library and Union Theological Seminary Library.Item at 255:E.172, no. 22 identified as A3339 (number cancelled in Wing 2nd ed.).eebo-0216Great BritainHistoryCharles I, 1625-1649SourcesSquire Johnca. 1588-1653.1003027EAAEAAm/cUMIWaOLNBOOK996383796403316An answer to a printed paper entituled Articles exhibited in Parliament against Mr. John Sqvier, viccar of Saint Leonard Shoreditch, August 7, 16412378735UNISA03387nam 22005055 450 991029840710332120200702011749.03-319-98071-810.1007/978-3-319-98071-3(CKB)4100000007127536(MiAaPQ)EBC5592914(DE-He213)978-3-319-98071-3(PPN)232472556(EXLCZ)99410000000712753620181108d2018 u| 0engurcnu||||||||txtrdacontentcrdamediacrrdacarrierFlow Cytometry Basics for the Non-Expert /by Christine Goetz, Christopher Hammerbeck, Jody Bonnevier1st ed. 2018.Cham :Springer International Publishing :Imprint: Springer,2018.1 online resource (xvii, 219 pages)Techniques in Life Science and Biomedicine for the Non-Expert,2367-11143-319-98070-X 1. Flow Cytometry: Definition, History, and Uses in Biotechnology -- 2. Physics of a Flow Cytometer -- 3. The Language of Flow Cytometry and Experimental Setup -- 4. Fluorochrome Choices for Flow Cytometry -- 5. Compensation -- 6. Primary and Seconardy Antibodies and Their Use in Flow Cytometry -- 7. Experimental Considerations with Data Sets as Examples -- 8. Cell Enrichment -- 9. Surface and Intracellular Staining Protocols for Flow Cytometry -- 10. Troubleshooting.Flow cytometry allows for the measurement of different aspects of a cell or particles in real time. Applications include quantifying various types of T cells to determine the presence of various immune disorders, such as whether HIV has progressed to AIDS, studying the dynamics of immune signaling in cells to detect the suppression of normal immune function, and analyzing biopsy specimens for tumors, to aid in cancer diagnosis and prognosis. In addition to simplifying the technique and using vocabulary accessible to those not trained in cell biology and immunology, the book includes the following 4 unqiue features: 1) Tips on how to set up an experiment for flow cytometry optimially 2) An expanded data set of flow staining including multiparameter flow cytometry 3) Detailed staining protocols for flow cytometry (ex. protocols optimized for transcription factors, secreted cytokines, phospho-antibodies, and surface antigens) 4) Section devoted to antibody development and conjugation.Techniques in Life Science and Biomedicine for the Non-Expert,2367-1114ImmunoglobulinsDiagnosis, LaboratoryAntibodieshttps://scigraph.springernature.com/ontologies/product-market-codes/B14010Laboratory Medicinehttps://scigraph.springernature.com/ontologies/product-market-codes/B15007Immunoglobulins.Diagnosis, Laboratory.Antibodies.Laboratory Medicine.574.87028Goetz Christineauthttp://id.loc.gov/vocabulary/relators/aut1059859Hammerbeck Christopherauthttp://id.loc.gov/vocabulary/relators/autBonnevier Jodyauthttp://id.loc.gov/vocabulary/relators/autBOOK9910298407103321Flow Cytometry Basics for the Non-Expert2508889UNINA