03943nam 22007215 450 991029832900332120200703091337.03-0348-0856-910.1007/978-3-0348-0856-9(CKB)3710000000218675(EBL)1802959(SSID)ssj0001338289(PQKBManifestationID)11735551(PQKBTitleCode)TC0001338289(PQKBWorkID)11337752(PQKB)11429357(MiAaPQ)EBC1802959(DE-He213)978-3-0348-0856-9(PPN)180627473(EXLCZ)99371000000021867520140805d2014 u| 0engur|n|---|||||txtccrFluorescent Methods for Molecular Motors /edited by Christopher P. Toseland, Natalia Fili1st ed. 2014.Basel :Springer Basel :Imprint: Springer,2014.1 online resource (306 p.)Experientia Supplementum,1664-431X ;105Description based upon print version of record.1-322-13356-5 3-0348-0855-0 Includes bibliographical references at the end of each chapters.Fluorescence and Labelling: How to choose and what to do -- Fluorescent biosensors: design and application to motor proteins -- Rapid Reaction Kinetic Techniques -- Fluorescence to study the ATPase mechanism of Motor Proteins -- Use of pyrene labelled actin to probe actin myosin interactions; kinetic and equilibrium studies -- Fluorescent methods to study transcription initiation and transition into elongation -- Single-molecule and single-particle imaging of molecular motors in vitro and in vivo -- Fluorescence methods in the investigation of the DEAD-box helicase mechanism -- Use of Fluorescent Techniques to Study the In Vitro Movement of Myosins -- Fluorescence Tracking of Motor proteins in vitro -- Measuring Transport of Motor Cargos -- Measuring two at the same time: Combining Magnetic Tweezers with Single-Molecule FRET -- Using fluorescence to study actomyosin in yeasts.This book focuses on the application of fluorescence to study motor proteins (myosins, kinesins, DNA helicases and RNA polymerases). It is intended for a large community of biochemists, biophysicists and cell biologists who study a diverse collection of motor proteins. It can be used by researchers to gain an insight into their first experiments, or by experienced researchers who are looking to expand their research to new areas. Each chapter provides valuable advice for executing the experiments, along with detailed background knowledge in order to develop own experiments.Experientia Supplementum,1664-431X ;105Proteins Cell biologyBiophysicsBiological physicsProtein Sciencehttps://scigraph.springernature.com/ontologies/product-market-codes/L14040Cell Biologyhttps://scigraph.springernature.com/ontologies/product-market-codes/L16008Biological and Medical Physics, Biophysicshttps://scigraph.springernature.com/ontologies/product-market-codes/P27008Proteins .Cell biology.Biophysics.Biological physics.Protein Science.Cell Biology.Biological and Medical Physics, Biophysics.574.19245572.64Toseland Christopher Pedthttp://id.loc.gov/vocabulary/relators/edtFili Nataliaedthttp://id.loc.gov/vocabulary/relators/edtMiAaPQMiAaPQMiAaPQBOOK9910298329003321Fluorescent Methods for Molecular Motors2539462UNINA