04270nam 22007215 450 991029832080332120200706082902.094-017-8819-710.1007/978-94-017-8819-9(CKB)3710000000114553(EBL)1731570(OCoLC)881443479(SSID)ssj0001237254(PQKBManifestationID)11735395(PQKBTitleCode)TC0001237254(PQKBWorkID)11258140(PQKB)11480825(MiAaPQ)EBC1731570(DE-He213)978-94-017-8819-9(iGPub)SPNA0035491(PPN)17878530X(EXLCZ)99371000000011455320140519d2014 u| 0engur|n|---|||||txtccrStudies of Pluripotency in Embryonic Stem Cells and Induced Pluripotent Stem Cells[electronic resource] /by Xiaoyang Zhao1st ed. 2014.Dordrecht :Springer Netherlands :Imprint: Springer,2014.1 online resource (104 p.)Springer Theses, Recognizing Outstanding Ph.D. Research,2190-5053"Doctoral thesis accepted by University of Chinese Academy of Sciences, Beijing, China."94-017-8818-9 Includes bibliographical references at the end of each chapters.Abstract -- Introduction -- Establishment of highly efficient somatic cell reprogramming system to generate iPSC lines- Establishment of highly efficient somatic cell reprogramming system and establishment of iPSC lines -- Pluripotency of iPSC and underlining mechanism -- Developmental potential of mouse iPSC -- Conclusions.Stem cells have the ability to differentiate into all types of cells within the body, thus have great therapeutic potential for regenerative medicine to treat complicated disorders, like Parkinson’s disease and spinal cord injury. There will also be many applications in drug development. However, several roadblocks, such as safety issues and low efficiency of pluripotent stem cell (PSC) line derivation need to be resolved before their clinical application. This thesis focuses on these two areas, so as to find methods to overcome the limitation. It covers deriving embryonic stem cells (ESCs) from several different species, and reports an efficient system to generate induced pluripotent stem cells (iPSCs), and the first iPSC mice in the world. The results in this thesis confirm that somatic cells can be fully reprogrammed with the four Yamanaka factors. In addition, we have found that the Dlk1-Dio3 region can be a potential molecular marker to distinguish the fully reprogrammed iPSCs from partially reprogrammed ones. All of these results will help improve the safety of PSCs in the clinical applications, and increase the current low induction efficiency of their production.Springer Theses, Recognizing Outstanding Ph.D. Research,2190-5053Stem cellsCell cultureRegenerative medicineTissue engineeringDevelopmental biologyStem Cellshttps://scigraph.springernature.com/ontologies/product-market-codes/L16010Cell Culturehttps://scigraph.springernature.com/ontologies/product-market-codes/L16020Regenerative Medicine/Tissue Engineeringhttps://scigraph.springernature.com/ontologies/product-market-codes/L16080Developmental Biologyhttps://scigraph.springernature.com/ontologies/product-market-codes/L18000Stem cells.Cell culture.Regenerative medicine.Tissue engineering.Developmental biology.Stem Cells.Cell Culture.Regenerative Medicine/Tissue Engineering.Developmental Biology.571.6Zhao Xiaoyangauthttp://id.loc.gov/vocabulary/relators/aut1058752BOOK9910298320803321Studies of Pluripotency in Embryonic Stem Cells and Induced Pluripotent Stem Cells2502161UNINA