05316nam 2200649 450 991028495220332120180629182913.01-78924-463-31-78064-452-3(CKB)3800000000006896(EBL)1834013(SSID)ssj0001368958(PQKBManifestationID)12545629(PQKBTitleCode)TC0001368958(PQKBWorkID)11289495(PQKB)10145939(MiAaPQ)EBC1834013(PPN)250728931(EXLCZ)99380000000000689620141121h20142014 uy 0engur|n|---|||||txtccrTransgenic insects techniques and applications /Mark Q. Benedict, editorOxfordshire, England ;Boston, Massachusetts :CABI,2014.©20141 online resource (401 p.)CABI Biotechnology Series ;3Description based upon print version of record.1-78064-451-5 Includes bibliographical references at the end of each chapters and index.Contents; Contributors; Acknowledgements; Preface; PART 1: GERMLINE TRANSFORMATION TECHNOLOGY; 1 Transposons for Insect Transformation; 1.1 Transposable Elements; 1.2 DNA Transposons; 1.3 Transposons with Activity in Insects; 1.4 Summary; References; 2 Transposon-Based Technologies for Insects; 2.1 Transposon-Based Technologies; 2.2 Mutagenesis; 2.3 Germ-Line Transformation; 2.4 Modular Expression Systems; 2.5 Cell/Genetic Ablation; 2.6 Transgene Mis-expression; 2.7 Transgenic Gene Silencing; 2.8 Site-Specifi c Recombination; 2.9 Genetic Sensors; 2.10 Conclusions; References3 Sex-, Tissue- and Stage-Specifi c Transgene Expression3.1 Introduction; 3.2 Gene Regulation in Insects; 3.3 Th e Basic Genetic Construct; 3.4 Controlling for Position Eff ects; 3.5 General Considerations for Promoter Choice; 3.6 Sex-Specifi c Transgene Expression; 3.7 Tissue-Specifi c Expression; 3.8 Stage-Specifi c Expression; 3.9 Future Prospects; 3.10 Applications of Sex-, Tissue- and Stage-Specifi c Transgene Expression; References; 4 Docking Systems for Site-Directed Transgene Integration; 4.1 Background and Introduction4.2 Site-Specifi c Transgenesis - Generation of Phase 1 Docking Strains4.3 Site-Specifi c Transgenesis - Generation of Phase 2 Integrations; 4.4 Recombinase-Mediated Cassette Exchange; 4.5 Future Developments in the Use of Docking Systems; 4.6 Docking Systems Combined with Transposon Stabilization Strategies; 4.7 Integration of Large, Complex Transgene Constructs; 4.8 Construction of Complex Transgenes by Sequential Use of Integrases; References; 5 Inducible and Repressible Systems for Transgene Expression; 5.1 Introduction; 5.2 Naturally Occurring Systems of Conditional Expression5.3 Synthetic Systems5.4 Conclusions; References; 6 Sex Ratio Manipulation for Insect Population Control; 6.1 Introduction; 6.2 Overview of Applications and General Principles; 6.3 Meiotic Drive; 6.4 Sex-Specifi c Lethality; 6.5 Manipulation of Sex Determination Mechanisms; 6.6 Conclusions; References; 7 Conditional Dominant Lethals - RIDL; 7.1 Re-engineering the Sterile Insect Technique; 7.2 Sterile Insects and Genetic Control; 7.3 Engineered Traits; 7.4 Integrated Pest Management; 7.5 Resistance Management; 7.6 Molecular Designs; 7.7 Choosing an Eff ector; 7.8 Choice of Switch7.9 Strain Performance7.10 Penetrance; 7.11 Resistance; 7.12 Field Experience and Future Prospects; Notes; Acknowledgements; References; PART 2: APPLICATIONS OF TRANSGENIC INSECTS; 8 Tephritid Fruit Fly Transgenesis and Applications; 8.1 Introduction; 8.2 Transformation with the Minos Vector System; 8.3 Transformation with the piggyBac Vector System; 8.4 Transformation with the Hermes Vector System; 8.5 Marker Systems for Transformant Organismal and Tissue Detection in Tephritid Flies; 8.6 Post-integration Stabilization of Transposon Vectors in Tephritid Flies8.7 Site-Specifi c Genomic Targeting in TephritidsInsect transgenesis promises improvements in agriculture, pharmaceuticals and public health. Many important insects can now be routinely transformed with effectors that have useful applications. Agriculture presents the largest market for transgenic insects and has a foundational history of success with sterile insect technique for control of pests including Mediterranean fruit flies and screwworms. Biotechnology will contribute superior markers, suppressible sterility and sex-conversion. Public health is also seeing transgenic mosquitoes developed which suppress natural populations and are inCABI biotechnology series ;3.InsectsGeneticsTransgenic animalsInsect pestsControlInsectsGenetics.Transgenic animals.Insect pestsControl.631.5233Benedict Mark Q.C.A.B. International,MiAaPQMiAaPQMiAaPQBOOK9910284952203321Transgenic insects2793120UNINA