03205nam 22006855 450 991025405800332120200629214323.03-319-09558-710.1007/978-3-319-09558-5(CKB)3710000000492514(EBL)4178155(SSID)ssj0001584190(PQKBManifestationID)16263230(PQKBTitleCode)TC0001584190(PQKBWorkID)14865605(PQKB)11030710(DE-He213)978-3-319-09558-5(MiAaPQ)EBC4178155(PPN)190536764(EXLCZ)99371000000049251420151017d2016 u| 0engur|n|---|||||txtccrSingle-Molecule Fluorescence Spectroscopy of the Folding of a Repeat Protein /by Sharona Cohen1st ed. 2016.Cham :Springer International Publishing :Imprint: Springer,2016.1 online resource (70 p.)Springer Theses, Recognizing Outstanding Ph.D. Research,2190-5053Description based upon print version of record.3-319-09557-9 Includes bibliographical references.Abstract -- Introduction -- Methods -- Results -- Discussion -- Summary and Future Plans.In this thesis single-molecule fluorescence resonance energy transfer (FRET) spectroscopy was used to study the folding of a protein that belongs to the large and important family of repeat proteins. Cohen shows that the dynamics of the expanded conformations is likely to be very fast, suggesting a spring-like motion of the whole chain. The findings shed new light on the elasticity of structure in repeat proteins, which is related to their function in binding multiple and disparate partners. This concise research summary provides useful insights for students beginning a PhD in this or a related area, and researchers entering this field.Springer Theses, Recognizing Outstanding Ph.D. Research,2190-5053Bioorganic chemistrySpectroscopyBiophysicsBiological physicsBioorganic Chemistryhttps://scigraph.springernature.com/ontologies/product-market-codes/C19010Spectroscopy/Spectrometryhttps://scigraph.springernature.com/ontologies/product-market-codes/C11020Biological and Medical Physics, Biophysicshttps://scigraph.springernature.com/ontologies/product-market-codes/P27008Bioorganic chemistry.Spectroscopy.Biophysics.Biological physics.Bioorganic Chemistry.Spectroscopy/Spectrometry.Biological and Medical Physics, Biophysics.543.56Cohen Sharonaauthttp://id.loc.gov/vocabulary/relators/aut1061392MiAaPQMiAaPQMiAaPQBOOK9910254058003321Single-Molecule Fluorescence Spectroscopy of the Folding of a Repeat Protein2518692UNINA