05437nam 2200637Ia 450 991014455960332120170814171740.01-280-92167-697866109216763-527-61092-83-527-61093-6(CKB)1000000000377144(EBL)481506(OCoLC)173135610(SSID)ssj0000156119(PQKBManifestationID)11158482(PQKBTitleCode)TC0000156119(PQKBWorkID)10134196(PQKB)10823756(MiAaPQ)EBC481506(EXLCZ)99100000000037714420060529d2007 uy 0engur|n|---|||||txtccrFlow cytometry with plant cells[electronic resource] analysis of genes, chromosomes and genomes /edited by Jaroslav Doležel, Johann Greilhuber, and Jan SudaWeinheim Wiley-VCH ;Chichester John Wiley [distributor]20071 online resource (481 p.)Description based upon print version of record.3-527-31487-3 Includes bibliographical references and index.Flow Cytometry with Plant Cells; Contents; Preface; List of Contributors; 1 Cytometry and Cytometers: Development and Growth; Overview; 1.1 Origins; 1.2 From Absorption to Fluorescence, from Imaging to Flow; 1.2.1 Early Microspectrophotometry and Image Cytometry; 1.2.2 Fluorescence Microscopy and the Fluorescent Antibody Technique; 1.2.3 Computers Meet Cytometers: The Birth of Analytical Flow Cytometry; 1.2.4 The Development of Cell Sorting; 1.3 The Growth of Multiparameter Flow Cytometry; 1.4 Bench-tops and Behemoths: Convergent Evolution; 1.5 Image Cytometry: New Beginnings?; References2 Principles of Flow CytometryOverview; 2.1 Introduction; 2.2 A Brief History of Flow Cytometry; 2.3 Components of a Flow Cytometer; 2.3.1 Fluidics; 2.3.2 Optics; 2.3.3 Electronic Systems; 2.4 Flow Cytometric Informatics; 2.5 Spectral Compensation; 2.6 Cell Sorting; 2.7 Calibration Issues; 2.8 Conclusions; References; 3 Flow Cytometry with Plants: an Overview; Overview; 3.1 Introduction; 3.2 Fluorescence is a Fundamental Parameter; 3.3 Pushing Plants through the Flow Cytometer; 3.3.1 Difficulties with Plants and their Cells; 3.3.2 Protoplasts are somewhat ""Easier"" than Intact Cells3.3.3 Going for Organelles3.4 Application of Flow Cytometry in Plants; 3.4.1 Microspores and Pollen; 3.4.2 Protoplasts; 3.4.2.1 Physiological Processes; 3.4.2.2 Secondary Metabolites; 3.4.2.3 Gene Expression; 3.4.2.4 Somatic Hybrids; 3.4.2.5 DNA Transfection; 3.4.3 Cell Nuclei; 3.4.3.1 Ploidy Levels; 3.4.3.2 Aneuploidy; 3.4.3.3 B Chromosomes; 3.4.3.4 Sex Chromosomes; 3.4.3.5 Cell Cycle and Endopolyploidy; 3.4.3.6 Reproductive Pathways; 3.4.3.7 Nuclear Genome Size; 3.4.3.8 DNA Base Content; 3.4.3.9 Chromatin Composition; 3.4.3.10 Sorting of Nuclei; 3.4.4 Mitotic Chromosomes; 3.4.5 Chloroplasts3.4.6 Mitochondria3.4.7 Plant Pathogens; 3.4.8 Aquatic Flow Cytometry; 3.5 A Flow Cytometer in Every Laboratory?; 3.6 Conclusions and Future Trends; References; 4 Nuclear DNA Content Measurement; Overview; 4.1 Introduction; 4.2 Nuclear DNA Content: Words, Concepts and Symbols; 4.2.1 Replication-Division Phases; 4.2.2 Alternation of Nuclear Phases; 4.2.3 Generative Polyploidy Levels; 4.2.4 Somatic Polyploidy; 4.3 Units for Presenting DNA Amounts and their Conversion Factors; 4.4 Sample Preparation for Flow Cytometric DNA Measurement; 4.4.1 Selection of the Tissue; 4.4.2 Reagents and Solutions4.4.2.1 Isolation Buffers and DNA Staining4.5 Standardization; 4.5.1 Types of Standardization; 4.5.2 Requirement of Internal Standardization - a Practical Test; 4.5.3 Choice of the Appropriate Standard Species; 4.5.3.1 Biological Similarity; 4.5.3.2 Genome Size; 4.5.3.3 Nature of the Standard; 4.5.3.4 Availability; 4.5.3.5 Cytological Homogeneity; 4.5.3.6 Accessibility; 4.5.3.7 Reliability of C-Values; 4.5.4 Studies on Plant Standards; 4.5.5 Suggested Standards; 4.6 Fluorescence Inhibitors and Coatings of Debris; 4.6.1 What are Fluorescence Inhibitors and Coatings of Debris?4.6.2 Experiments with Tannic AcidTargeted at beginners as well as experienced users, this handy reference explains the benefits and uses of flow cytometery in the study of plants and their genomes. Following a brief introduction that highlights general considerations when analyzing plant cells by flow cytometric methods, the book goes on to discuss examples of application in plant genetics, genomic analysis, cell cycle analysis, marine organism analysis and breeding studies.With its list of general reading and a glossary of terms, this first reference on FCM in plants fills a real gap by providing first-hand practical hinFlow cytometryPlant cells and tissuesElectronic books.Flow cytometry.Plant cells and tissues.571.62Doležel Jaroslav863264Greilhuber Johann863265Suda Jan863266MiAaPQMiAaPQMiAaPQBOOK9910144559603321Flow cytometry with plant cells1926984UNINA05202oas 2201645 a 450 991014393440332120251105213014.01795-990X(CONSER) 2009250609(CKB)954925520658(OCoLC)60626411(DE-599)ZDB2083318-0(TW-Airiti)jkey=03553140(TW-Airiti)03553140(MiAaPQ)37939(EXLCZ)9995492552065820050614a19759999 uy engur|||||||||||txtrdacontentcrdamediacrrdacarrierScandinavian journal of work, environment & healthHelsinki, Finland National Board of Occupational Safety and Health, Sweden, Occupational Health Foundation, Finland, Swedish Medical Society, Section for Environmental Health, Sweden, Work Research Institutes, Norway, Workers' Protection Fund, Denmark1975-Helsinki, Finland Nordic Association of Occupational Safety and Health (NOROSH)Refereed/Peer-reviewedTitle from PDF cover (JSTOR, viewed Aug. 12, 2011).0355-3140 Scandinavian journal of work, environment and healthSJWEHScand J Work Environ HealthMedicine, IndustrialPeriodicalsEnvironmentally induced diseasesPeriodicalsWorkPhysiological aspectsPeriodicalsIndustrial hygienePeriodicalsEnvironmental healthOccupational health servicesEnvironmental HealthOccupational HealthOccupational Health ServicesEnvironmental ExposureEnvironmentWorkpsychologyMédecine du travailPériodiquesMaladies de l'environnementPériodiquesTravailAspect physiologiquePériodiquesHygiène industriellePériodiquesHygiène du milieuServices de médecine du travailExposition (Toxicologie)Occupational health servicesfast(OCoLC)fst01043207Environmental healthfast(OCoLC)fst00912999Environmentally induced diseasesfast(OCoLC)fst00913560Industrial hygienefast(OCoLC)fst00971122Medicine, Industrialfast(OCoLC)fst01015256WorkPhysiological aspectsfast(OCoLC)fst01180199ArbeidshygiënegttBeroepsziektengttPeriodicals.fastMedicine, IndustrialEnvironmentally induced diseasesWorkPhysiological aspectsIndustrial hygieneEnvironmental health.Occupational health services.Environmental Health.Occupational Health.Occupational Health Services.Environmental Exposure.Environment.Workpsychology.Médecine du travailMaladies de l'environnementTravailAspect physiologiqueHygiène industrielleHygiène du milieu.Services de médecine du travail.Exposition (Toxicologie)Occupational health services.Environmental health.Environmentally induced diseases.Industrial hygiene.Medicine, Industrial.WorkPhysiological aspects.Arbeidshygiëne.Beroepsziekten.616.9/803/05Sweden.Arbetarskyddsstyrelsen.TyöterveyssäätiöSvenska läkaresällskapetWork Research Institute (Norway)Työterveyslaitos (Finland)Arbejdsmiljøinstituttet (Denmark)Statens arbeidsmiljøinstitutt (Norway)Arbetslivsinstitutet (Sweden)Nordic CouncilOCLCSOCLCSMUQGAOBUFDNBNLGGCOCLCQLGGUX1TXJEYMOCLCQOCLCAOCLCFOCLCQOCLCOOCLCQZ5AOCLCOWY@OCLCOOCLCAU3WOCLCOOCLCAAPUMSOCLCOAU@OCLCOWYUOCLCOOCLCADLCOCLCOOCLCQOCLUABOCLCLOCLCQJOURNAL9910143934403321Scandinavian journal of work, environment & health1887602UNINA