05439nam 2200697 450 991014411740332120170810183332.01-281-94680-X97866119468073-527-62229-23-527-62230-6(CKB)1000000000549420(EBL)481336(OCoLC)277256524(SSID)ssj0000229680(PQKBManifestationID)11174950(PQKBTitleCode)TC0000229680(PQKBWorkID)10173121(PQKB)10369351(MiAaPQ)EBC481336(EXLCZ)99100000000054942020160819h20082008 uy 0engur|n|---|||||txtccrProteomics in practice a guide to successful experimental design /edited by Reiner Westermeier, Tom Naven, Hans-Rudolf HöpkerSecond, completely revised edition.Weinheim, Germany :WILEY-VCH Verlag GmbH & Co. KGaA,2008.©20081 online resource (504 p.)Description based upon print version of record.3-527-31941-7 Includes bibliographical references and index.Proteomics in Practice; Contents; Preface; Foreword; Abbreviations, Symbols, Units; Introduction; 1 History; 2 Critical Points; 2.1 Challenges of the Protein Samples; 2.2 Challenges of the Analysis Systems; 3 Proteomics Strategies; 3.1 Proteome Mapping; 3.2 Differential Analysis; 3.3 Time Point Experiments; 3.4 Verification of Targets or Biomarkers; 3.5 Integration of Results into Biological Context; 3.6 Systems Biology; 4 Concept of Experimental Planning; 4.1 Biological Replicates; 4.2 Pooling of Samples: Yes or No?; 4.3 Pre-fractionation of Samples: Yes or No?4.4 Which is the Best Workflow to Start With?Part I: Proteomics Technology; 1 Electrophoretic Techniques; 1.1 The Principle of Electrophoresis and Some Methodological Background; 1.1.1 Free Flow Electrophoretic Methods; 1.1.2 Gels for Electrophoretic Techniques; 1.1.3 Electroendosmosis Effects; 1.2 Polyacrylamide Gel Electrophoresis; 1.2.1 The Polyacrylamide Gel; 1.2.2 SDS Polyacrylamide Gel Electrophoresis; 1.2.3 Blue Native Electrophoresis; 1.2.4 Cationic Detergent Electrophoresis; 1.3 Blotting; 1.3.1 Electrophoretic Transfer; 1.3.2 Protein Detection on the Membrane1.4 Isoelectric Focusing1.4.1 Theoretical Background; 1.4.2 Preparation of IEF Gels; 1.4.3 Isoelectric Focusing in Proteomics; 1.5 Two-dimensional Electrophoresis; 1.5.1 Sample Preparation; 1.5.2 Pre-labeling of Proteins for Difference Gel Electrophoresis; 1.5.3 First Dimension: Isoelectric Focusing in IPG Strips; 1.5.4 Second Dimension: SDS Electrophoresis; 1.5.5 Detection of Protein Spots; 1.6 Image Analysis; 1.6.1 Image Acquisition; 1.6.2 Image Analysis and Evaluation; 1.6.3 Use of 2-D Electrophoresis Data; 1.7 Spot Handling; 1.7.1 Spot Picking; 1.7.2 Protein Cleavage2 Liquid Chromatography Techniques2.1 Basic Principles of Important Liquid Chromatography Techniques; 2.1.1 Ion Exchange Chromatography; 2.1.2 Reversed Phase Chromatography; 2.1.3 Affinity Chromatography; 2.1.4 Gel Filtration; 2.2 Strategic Approach and General Applicability; 2.3 Liquid Chromatography Techniques and Applications in Proteome Analysis; 2.3.1 Peptide Separation; 2.3.2 2DLC Peptide Separation; 2.3.3 Affinity Chromatography and LC-MS/MS; 2.3.4 Protein Pre-fractionation; 2.4 Practical Considerations and Application of LC-based Protein Pre-fractionation2.4.1 Sample Extraction and Preparation2.4.2 Experimental Setup; 2.4.3 Ion Exchange Chromatography and Protein Pre-fractionation; 2.4.4 Reversed Phase Chromatography and Protein Pre-fractionation; 2.4.5 Fraction Size and Number of Fractions; 2.5 Critical Review and Outlook; 3 Mass Spectrometry; 3.1 Ionization; 3.1.1 Matrix Assisted Laser Desorption Ionization; 3.1.2 Electrospray Ionization; 3.2 Ion Separation; 3.2.1 Time-of-Flight Analyzer; 3.2.2 Triple Quadrupole Analyzer; 3.2.3 Quadrupole Ion Trap; 3.2.4 Quadrupole Time-of-Flight; 3.2.5 Hybrid Triple Quadrupole Linear Ion Trap3.2.6 TOF/TOF AnalyzerStill the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC.ProteomicsHandbooks, manuals, etcProteinsAnalysisMolecular biologyElectronic books.ProteomicsProteinsAnalysis.Molecular biology.572.6572.64Westermeier ReinerNaven TomHöpker Hans-RudolfMiAaPQMiAaPQMiAaPQBOOK9910144117403321Proteomics in practice1984061UNINA01980nam0 22003973i 450 ANA045876420251003044039.020151116d1971 ||||0itac50 baitaitz01i xxxe z01nz01ncRDAcarrierMovimento sindacale e contrattazione collettiva1945-1970Francesco Drago ... [et al.]MilanoAngeli1971430 p.22 cmCollana di cultura sindacale1001LO100300842001 Collana di cultura sindacale1Contratti collettiviItalia1945-1970FIRLO1C535656I331.880922SINDACATI. Biografia collettiva23331.8909450904Contrattazione e controversie collettive. Italia. 20. secolo, 1900-199922Accordi collettiviContratti collettivi d'impiegoRapporti collettivi di lavoroContratti collettivi di lavoroAccordi collettivi di lavoroContratti collettiviAccordi collettiviContratti collettiviContratti collettivi d'impiegoContratti collettiviRapporti collettivi di lavoroContratti collettiviContratti collettivi di lavoroContratti collettiviAccordi collettivi di lavoroDrago, FrancescoLO1V133918070ITIT-00000020151116IT-BN0095 NAP 01POZZO LIB.Vi sono collocati fondi di economia, periodici di ingegneria e scienze, periodici di economia e statistica e altri fondi comprendenti documenti di economia pervenuti in dono. ANA0458764Biblioteca Centralizzata di Ateneo1 v. 01POZZO LIB.F. SANTI 234 0101 0060032535E VMA 1 v. (Precedente collocazione S 241)B 2022110920221109 01Movimento sindacale e contrattazione collettiva457251UNISANNIO