05238nam 2200673Ia 450 991014400830332120170810192822.01-282-37220-397866123722093-527-62021-43-527-62036-2(CKB)1000000000376040(EBL)481470(OCoLC)741346724(SSID)ssj0000307206(PQKBManifestationID)11238766(PQKBTitleCode)TC0000307206(PQKBWorkID)10244368(PQKB)10148525(MiAaPQ)EBC481470(EXLCZ)99100000000037604020060807d2006 uy 0engur|n|---|||||txtccrProtein degradationVolume 2The Ubiquitin-proteasome system[electronic resource] /R. John Mayer, Aaron Ciechanover, Martin Rechsteiner, edsWeinheim Wiley-VCHc20061 online resource (302 p.)Protein Degradation ;v.9Description based upon print version of record.3-527-31130-0 Includes bibliographical references and index.Protein Degradation; Contents; Preface; List of Contributors; 1 Molecular Chaperones and the Ubiquitin-Proteasome System; 1.1 Introduction; 1.2 A Biomedical Perspective; 1.3 Molecular Chaperones: Mode of Action and Cellular Functions; 1.3.1 The Hsp70 Family; 1.3.2 The Hsp90 Family; 1.3.3 The Small Heat Shock Proteins; 1.3.4 Chaperonins; 1.4 Chaperones: Central Players During Protein Quality Control; 1.5 Chaperones and Protein Degradation; 1.6 The CHIP Ubiquitin Ligase: A Link Between Folding and Degradation Systems1.7 Other Proteins That May Influence the Balance Between Chaperone-assisted Folding and Degradation1.8 Further Considerations; 1.9 Conclusions; References; 2 Molecular Dissection of Autophagy in the Yeast Saccharomyces cerevisiae; 2.1 Introduction; 2.2 Vacuoles as a Lytic Compartment in Yeast; 2.3 Discovery of Autophagy in Yeast; 2.4 Genetic Dissection of Autophagy; 2.5 Characterization of Autophagy-defective Mutants; 2.6 Cloning of ATG Genes; 2.7 Further Genes Required for Autophagy; 2.8 Selectivity of Proteins Degraded; 2.9 Induction of Autophagy; 2.10 Membrane Dynamics During Autophagy2.11 Monitoring Methods of Autophagy in the Yeast S. cerevisiae2.12 Function of Atg Proteins; 2.12.1 The Atg12 Protein Conjugation System; 2.12.2 The Atg8 System; 2.12.3 The Atg1 Kinase Complex; 2.12.4 Autophagy-specific PI3 Kinase Complex; 2.12.5 Other Atg Proteins; 2.13 Site of Atg Protein Functioning: The Pre-autophagosomal Structure; 2.14 Atg Proteins in Higher Eukaryotes; 2.15 Atg Proteins as Markers for Autophagy in Mammalian Cells; 2.16 Physiological Role of Autophagy in Multicellular Organisms; 2.17 Perspectives; References3 Dissecting Intracellular Proteolysis Using Small Molecule Inhibitors and Molecular Probes3.1 Introduction; 3.2 The Proteasome as an Essential Component of Intracellular Proteolysis; 3.3 Proteasome Structure, Function, and Localization; 3.4 Proteasome Inhibitors as Tools to Study Proteasome Function; 3.4.1 Peptide Aldehydes; 3.4.2 Lactacystin; 3.4.3 Peptide Epoxyketones; 3.4.4 Cyclic Peptides; 3.4.5 Peptide Boronates; 3.4.6 Peptide Vinyl Sulfones; 3.4.7 Peptide Vinyl Sulfones as Proteasomal Activity Probes3.4.8 Future Directions in the Development of Inhibitors of the Proteasome's Proteolytic Activities3.5 Assessing the Biological Role of the Proteasome With Inhibitors and Probes; 3.6 Proteasome-associated Components: The Role of N-glycanase; 3.7 A Link Between Proteasomal Proteolysis and Deubiquitination; 3.7.1 Reversal of Ub Modification; 3.7.2 Ubiquitin-specific Proteases; 3.7.3 USP Reactive Probes Correlate USP Activity With Proteasomal Proteolysis; 3.8 Future Developments and Final Remarks; Acknowledgments; Abbreviations; References4 MEKK1: Dual Function as a Protein Kinase and a Ubiquitin Protein LigaseThe second volume in a new series dedicated to protein degradation, this book discusses the mechanism and cellular functions of targeted protein breakdown via the ubiquitin pathway.Drawing on the combined knowledge of the world's leading protein degradation experts, this handy reference compiles information on the proteasome-mediated degradation steps of the ubiquitin pathway. In addition to proteasomal function and regulation, it also presents the latest results on novel members of the ubiquitin superfamily and their role in cellular regulation.Further volumes in the series cover the Protein DegradationProteinsMetabolismUbiquitinElectronic books.ProteinsMetabolism.Ubiquitin.572.76612.3/98Mayer R. J867090Ciechanover Aaron J867091Rechsteiner Martin867092MiAaPQMiAaPQMiAaPQBOOK9910144008303321Protein degradation1935375UNINA