05368nam 2200649Ia 450 991014396520332120170816123319.01-280-52060-497866105206023-527-60559-23-527-60554-1(CKB)1000000000375902(EBL)481617(OCoLC)68965702(SSID)ssj0000141995(PQKBManifestationID)11147216(PQKBTitleCode)TC0000141995(PQKBWorkID)10091591(PQKB)10032194(MiAaPQ)EBC481617(EXLCZ)99100000000037590220041129d2005 uy 0engur|n|---|||||txtccrDynamic studies in biology[electronic resource] phototriggers, photoswitches and caged biomolecules /edited by Maurice Goeldner, Richard GivensWeinheim Wiley-VCHc20051 online resource (587 p.)Description based upon print version of record.3-527-30783-4 Includes bibliographical references and index.Dynamic Studies in Biology Phototriggers, Photoswitches and Caged Biomolecules; Contents; Foreword; Preface; List of Authors; 1 Photoremovable Protecting Groups Used for the Caging of Biomolecules; 1.1 2-Nitrobenzyl and 7-Nitroindoline Derivatives; 1.1.1 Introduction; 1.1.1.1 Preamble and Scope of the Review; 1.1.1.2 Historical Perspective; 1.1.2 Synthetic Considerations; 1.1.3 Survey of Individual Caged Compounds and Caging Groups; 1.1.3.1 2-Nitrobenzyl Cages; 1.1.3.1.1 Mechanistic Aspects of Photocleavage and By-Product Reactions of 2-Nitrobenzyl Cages1.1.3.1.2 Representative Survey of Nitrobenzyl-Caged Compounds1.1.3.2 7-Nitroindoline Cages; 1.1.3.2.1 Mechanistic and Structural Aspects of Photochemical Cleavage of 1-Acyl-7-nitroindolines; 1.1.3.2.2 Survey of 7-Nitroindoline Caged Compounds; 1.1.4 Conclusion; References; 1.2 Coumarin-4-ylmethyl Phototriggers; 1.2.1 Introduction; 1.2.2 Spectroscopic and Photochemical Properties; 1.2.2.1 Overview; 1.2.2.2 Phototriggers; 1.2.2.2.1 MCM Groups: 7-Alkoxy-Substituted Coumarins; 1.2.2.2.2 DMCM Groups: 6,7-Dialkoxy-Substituted Coumarins; 1.2.2.2.3 Bhc Groups: 6-Bromo-7-alkoxy-Substituted Coumarins1.2.2.2.4 DEACM Groups: 7-Dialkylamino-Substituted Coumarins1.2.2.3 Target Molecules; 1.2.2.3.1 Phosphates; 1.2.2.3.2 Carboxylates and Sulfates; 1.2.2.3.3 Amines; 1.2.2.3.4 Alcohols and Phenols; 1.2.2.3.5 Carbonyl Compounds; 1.2.3 Synthesis; 1.2.3.1 Synthesis of Precursor Molecules (Caging Agents); 1.2.3.1.1 Phosphates; 1.2.3.1.2 Carboxylates; 1.2.3.1.3 Amines; 1.2.3.1.4 Alcohols and Phenols; 1.2.4 Applications; 1.2.5 Conclusion and Perspective; References; 1.3 p-Hydroxyphenacyl: a Photoremovable Protecting Group for Caging Bioactive Substrates; 1.3.1 Introduction and History1.3.2 p-Hydroxyphenacyl1.3.2.1 General Physical and Spectroscopic Properties; 1.3.2.2 Synthesis of pHP-Caged Substrates; 1.3.3 Mechanistic Studies; 1.3.3.1 A Triplet "Photo-Favorskii" Rearrangement; 1.3.3.2 Role of the Triplet Phenol; 1.3.3.3 Correlation of the (3)pK(a) with the Quantum Efficiency; 1.3.4 Applications; 1.3.4.1 Neurotransmitter Release; 1.3.4.2 Peptide Release; 1.3.4.3 Nucleotide Release; 1.3.4.4 Enzyme Photoswitches; 1.3.5 Advantages and Limitations; References; 1.4 Caging of ATP and Glutamate: a Comparative Analysis; 1.4.1 Introduction1.4.2 General Properties for Caging Groups1.4.3 Caged-ATP; 1.4.3.1 Introduction; 1.4.3.2 Syntheses of the P(3)-caged ATP Derivatives; 1.4.3.3 General Properties of the Caged-ATP Molecules; 1.4.3.4 Conclusion; 1.4.4 Caged Glutamate; 1.4.4.1 Introduction; 1.4.4.2 Syntheses of the Caged Glutamate Derivatives; 1.4.4.3 General Properties of the Caged-Glutamate Molecules; 1.4.4.4 Conclusion; Abbreviations; References; 2 Mechanistic Overview of Phototriggers and Cage Release; 2.1 Introduction; 2.2 Major Photoremovable Protecting Groups; 2.2.1 2-Nitrobenzyl (2-NB); 2.2.1.1 The Reactive Excited State2.2.1.2 The aci-Nitro IntermediateWith contributions by more than 30 expert researchers, this handbook covers the whole spectrum from chemistry to cell biology and from theory to application. In so doing, it deals with a broad range of topics from the chemistry and biophysics of caged compounds to their application in time-resolved studies, comparing the properties of different caging groups. The authors describe in detail light-activation of proteins as well as nucleic acids, while a special section is devoted to multiphoton phototriggers.A must-have for every biochemist, biophysicist and molecular biologist developing anPhotobiochemistryBiomoleculesSeparationProtective groups (Chemistry)Electronic books.Photobiochemistry.BiomoleculesSeparation.Protective groups (Chemistry)547.2Goeldner Maurice962288Givens Richard962289MiAaPQMiAaPQMiAaPQBOOK9910143965203321Dynamic studies in biology2181922UNINA01768nam 2200445 a 450 991069662340332120080617134457.0(CKB)5470000002380630(OCoLC)232154343(EXLCZ)99547000000238063020080617d2008 ua 0engurmn|||||||||txtrdacontentcrdamediacrrdacarrierTraffic safety[electronic resource] improved reporting and performance measures would enhance evaluation of high-visibility campaigns : report to the Chairman, Committee on Transportation and Infrastructure, House of Representatives[Washington, D.C.] :U.S. Govt. Accountability Office,[2008]ii, 42 pages digital, PDF fileTitle from title screen (viewed on June 2, 2008)."April 2008."Paper version available from: U.S. Govt. Accountability Office, 441 G St., NW, Rm. LM, Washington, D.C. 20548."GAO-08-477."Includes bibliographical references.Traffic safety Mass media in traffic safetyUnited StatesAutomobilesUnited StatesSeat beltsDrunk drivingUnited StatesPreventionTraffic safetyUnited StatesEvaluationMass media in traffic safetyAutomobilesSeat belts.Drunk drivingPrevention.Traffic safetyEvaluation.United States.Congress.House.Committee on Transportation and Infrastructure.GPOGPOBOOK9910696623403321Traffic safety1930480UNINA