05194nam 22006134a 450 991014396290332120210104162156.01-280-85416-297866108541653-527-60766-81-61583-208-43-527-60708-09783527312320(CKB)1000000000375908(EBL)481785(OCoLC)70113405(SSID)ssj0000118310(PQKBManifestationID)11133249(PQKBTitleCode)TC0000118310(PQKBWorkID)10053081(PQKB)11166711(MiAaPQ)EBC481785(EXLCZ)99100000000037590820060525d2005 uy 0engur|n|---|||||txtccrCarrier-bound immobilized enzymes[electronic resource] principles, applications and design /Linqiu CaoWeinheim Wiley-VCHc20051 online resource (581 p.)Description based upon print version of record.3-527-31232-3 Includes bibliographical references and index.Carrier-bound Immobilized Enzymes; Foreword; Contents; 1 Introduction: Immobilized Enzymes: Past, Present and Prospects; 1.1 Introduction; 1.2 The Past; 1.2.1 The Early Days (1916-1940s); 1.2.2 The Underdeveloped Phase (1950s); 1.2.3 The Developing Phase (1960s); 1.2.4 The Developed Phase (1970s); 1.2.5 The Post-developed Phase (1980s); 1.2.6 Rational Design of Immobilized Enzymes (1990s-date); 1.3 Immobilized Enzymes: Implications from the Past; 1.3.1 Methods of Immobilization; 1.3.2 Diversity versus Versatility; 1.3.3 Complimentary versus Alternative1.3.4 Modification versus Immobilization1.3.4.1 Enhanced Stability; 1.3.4.2 Enhanced Activity; 1.3.4.3 Improved Selectivity; 1.4 Prospective and Future Development; 1.4.1 The Room for Further Development; 1.4.2 An Integration Approach; 1.5 References; 2 Adsorption-based Immobilization; 2.1 Introduction; 2.2 Classification of Adsorption; 2.3 Principles Involved in Absorptive Enzyme Immobilization; 2.3.1 Monolayer Principle; 2.3.2 Stabilization Principle; 2.3.3 Enzyme Distribution; 2.4 Requirement of the Carriers; 2.4.1 Physical Requirements; 2.4.1.1 Pore-size and Available Surface2.4.1.2 Internal Structure2.4.1.3 Density of Binding Functionality; 2.4.1.4 Particle Size; 2.4.2 Chemical Nature of the Carriers; 2.4.2.1 Nature of Binding Functionality; 2.4.2.2 The Role of the Spacer; 2.4.2.3 The Nature of the Backbone; 2.5 Factors Which Dictate Enzyme Catalytic Performance; 2.5.1 Activity; 2.5.1.1 Diffusion-controlled Activity; 2.5.1.2 Conformation-controlled Activity; 2.5.1.3 Substrate-controlled Activity; 2.5.1.4 Loading-controlled Activity; 2.5.1.5 Medium-dependent Activity; 2.5.1.6 Microenvironment-dependent Activity; 2.5.1.7 Carrier Nature-dependent Activity2.5.1.8 Enzyme Nature-dependent Activity2.5.1.9 Additive-dependent Activity; 2.5.1.10 Hydrophilicity-dependent Activity; 2.5.1.11 Orientation-determined Activity; 2.5.1.12 Binding Nature-controlled Enzyme Activity; 2.5.1.13 Binding Density-controlled Enzyme Activity; 2.5.1.14 Reactor-dependent Activity; 2.5.1.15 Pore-size-dependent Activity; 2.5.1.16 Water-activity-dependent Activity; 2.5.2 Stability; 2.5.2.1 Conformation-controlled Stability; 2.5.2.2 Confinement-controlled Stability; 2.5.2.3 Enzyme Loading-dependent Stability; 2.5.2.4 Diffusion-controlled Stability2.5.2.5 Cross-linking-dependent Stability2.5.2.6 Carrier Nature-controlled Stability; 2.5.2.7 Aquaphilicity-controlled Stability; 2.5.2.8 Medium-controlled Stability; 2.5.2.9 Temperature-dependent Stability; 2.5.2.10 Microenvironment-controlled Stability; 2.5.2.11 Binding Nature-controlled Enzyme Stability; 2.5.2.12 Binding Density-controlled Enzyme Stability; 2.5.2.13 Additive-dependent Stability; 2.5.2.14 Enzyme Orientation-dependent Stability; 2.5.2.15 Enzyme-dependent Stability; 2.5.3 Selectivity; 2.5.3.1 Conformation-controlled Selectivity; 2.5.3.2 Diffusion-controlled Selectivity2.5.3.3 Binding Functionality-controlled SelectivityThe first systematic overview of this key technique since the early 1990s, this authoritative reference is the only handbook available to include all recent developments. The author draws on his wide-ranging experience in both academia and industry to systematically cover all types of enzyme immobilization methods, such as adsorption-based and covalent immobilization, as well as enzyme entrapment and encapsulation. Throughout, a careful review of materials and techniques for the generation of functional immobilized enzymes benefits both developers and users of carrier-bound enzymes.A mustImmobilized enzymesElectronic books.Immobilized enzymes.572.7Cao Linqiu863246MiAaPQMiAaPQMiAaPQBOOK9910143962903321Carrier-bound immobilized enzymes1926966UNINA