04218nam 22006014a 450 991014367610332120170816122204.01-280-90090-397866109009090-470-51161-30-470-51159-1(CKB)1000000000357047(EBL)297472(OCoLC)476071930(SSID)ssj0000174830(PQKBManifestationID)11922842(PQKBTitleCode)TC0000174830(PQKBWorkID)10188852(PQKB)11113400(MiAaPQ)EBC297472(PPN)152365346(EXLCZ)99100000000035704720070319d2007 uy 0engur|n|---|||||txtccrHuman embryonic stem cells[electronic resource] the practical handbook /editors, Stephen Sullivan, Chad A. Cowan and Kevin EgganChichester, West Sussex, England ;Hoboken, NJ John Wiley & Sonsc20071 online resource (430 p.)Description based upon print version of record.0-470-03356-8 Includes bibliographical references and index.Organization and good aseptic technique in the human embryonic stem cell laboratory / Minal J. Patel, Emma L. Stephenson and Stephen L. Minger -- Sourcing human embryonic stem cell lines / Glyn Stacey -- Culture of human embryos for stem cell derivation / R. Douglas Powers and Jeannine Witmyer -- Derivation of human embryonic stem cell lines / Stephen Sullivan ... [et al.] -- Standard culture of human embryonic stem cells / Jeremy M. Crook, Rachel Horne and Alan Colman -- Chemically-defined culture of human embryonic stem cells / Julie Hsu Clark and Sheng Ding -- Phenotypic analysis of human embryonic stem cells / Jonathan S. Draper, Cheryle A. Séguin and Peter W. Andrews -- Genetic and epigenetic analysis of human embryonic stem cells / Laurie A. Boyer, Rudolf Jaenisch and Maisam Mitalipova -- In vivo characterization of human embryonic stem cells / Scott A. Noggle, Francesca M. Spagnoli and Ali H. Brivanlou -- In vitro differentiation of human embryonic stem cells / A. Henry Sathananthan and Alan Trounson -- Differentiation of human embryonic stem cells into extraembryonic cell types / Cheryle A. Séguin and Jonathan S. Draper -- Directed differentiation of human embryonic stem cells into endoderm / Kenji Osafune ... [et al.] -- Directed differentiation of human embryonic stem cells into mesoderm / Christine Mummery ... [et al.] -- Directed differentiation of human embryonic stem cells into ectoderm / Emily A. Davis ... [et al.] -- Gene targeting in human embryonic stem cells : knock out and knock in by homologous recombination / Thomas P. Zwaka -- RNA interference in human embryonic stem cells / M. William Lensch, Asmin Tulpule and Holm Zaehres -- Generation of human gene reporters using bacterial artificial chromosome recombineering / Andrew J. Washkowitz and David A. Shaywitz.With this valuable practical guide, three members of the Harvard Stem Cell Institute have compiled and edited the definite handbook for the exciting new field of human embryonic stem cell research. The editors have gathered protocols from scientists with extensive reputation and expertise, describing and comparing currently used techniques for the culture of human stem cells and discussing the strengths and weaknesses of the different approaches. Human Embryonic Stem Cells: The Practical Handbook contains the first centralised collection of methods used in human embryonic stem cell biEmbryonic stem cellsResearchHuman embryoResearchEmbryonic stem cellsResearch.Human embryoResearch.612.64616.02774616/.02774Sullivan StephenDr.917805Cowan Chad A917806Eggan Kevin917807MiAaPQMiAaPQMiAaPQBOOK9910143676103321Human embryonic stem cells2057874UNINA01022nam1 22002653i 450 VAN0010992620241114014333.88720170619d1786 |0itac50 baitaIT||||||||| |||||||||Dissertazione sull'incertezza de' primi cinque secoli della storia romana opera di Luigi di Beaufort ... Traduzione dal francese. Parte prima [-seconda]Napolipresso i fratelli Roland17862 volumi8°001VAN001099282001 1VAN00109927Dissertation sur l'incertitude des cinq premiers.1465445NapoliVANL000005BeaufortLouis deVANV050659483668Roland fratelliVANV085042Jovene <editore>VANV107888650de Beaufort, LouisBeaufort, Louis deVANV058955ITSOL20241115RICAVAN00109926Dissertation sur l'incertitude des cinq premiers1465445UNICAMPANIA