05181nam 2200637Ia 450 991014312950332120170810175612.01-282-29205-697866122920573-527-62668-93-527-62669-7(CKB)1000000000767195(EBL)481485(OCoLC)437416793(SSID)ssj0000137586(PQKBManifestationID)11159659(PQKBTitleCode)TC0000137586(PQKBWorkID)10088690(PQKB)11399282(MiAaPQ)EBC481485(PPN)194575683(EXLCZ)99100000000076719520080107d2009 uy 0engur|n|---|||||txtccrDetection of highly dangerous pathogens[electronic resource] microarray methods for BSL 3 and BSL 4 agents /edited by Tanja Kostic, Patrick Butaye, and Jacques SchrenzelWeinheim Wiley-VCH20091 online resource (193 p.)Description based upon print version of record.3-527-32275-2 Includes bibliographical references and index.Detection of Highly Dangerous Pathogens: Microarray Methods for the Detection of BSL 3 and BSL 4 Agents; Contents; This Publication is Supported by COST; Preface; List of Contributors; 1 Introduction to Microarray-Based Detection Methods; 1.1 Introduction to Microarray Technology; 1.2 Technical Aspects of Microarray Technology; 1.2.1 Probes; 1.2.1.1 Genome Fragments; 1.2.1.2 PCR Products; 1.2.1.3 Oligonucleotide Probes; 1.2.2 Substrates for Printing; 1.2.2.1 Slides with Poly-L-lysine Coating; 1.2.2.2 Slides with Amino Silane Coating; 1.2.2.3 Slides with Aldehyde Coating1.2.2.4 Slides with Epoxy Coating1.2.2.5 Proprietary Surface Chemistries; 1.2.2.6 Probe Spacers; 1.2.3 Targets for Microarray Analysis; 1.2.3.1 Target Amplifications and Sensitivity Issues; 1.2.3.2 Labeling of the Targets; 1.2.3.3 Hybridization and Wash Conditions; 1.2.4 Classical Commercially Available Microarray Formats; 1.2.4.1 Spotting Approaches; 1.2.4.2 In Situ Synthesis; 1.2.5 Alternative Methods for Improving Microarray-Based Detection Sensitivity; 1.2.5.1 Resonance-Light Scattering (RLS); 1.2.5.2 Planar-Waveguide Technology (PWT); 1.2.5.3 Liquid Arrays1.2.5.4 Three-Dimensional Microarray Formats1.2.6 Marker Genes Used on MDMs; 1.3 Analysis and Quality Control Aspects; 1.4 Applications of Microarray Technology in Microbial Diagnostics; 1.4.1 Gene Expression Studies; 1.4.2 Comparative Genomic Hybridization (CGH); 1.4.3 Generic or Universal Microarrays; 1.4.4 Microarrays for Sequence Analysis; 1.4.5 Microbial Diagnostic Microarrays (MDMs); 1.5 Further Developments and New Perspectives Regarding Array Sensitivity and Specificity; 1.6 Conclusions; References; Part I: Methods; 2 Long Oligonucleotide Microarray-Based Microbial Detection2.1 Introduction2.2 Method; 2.2.1 DNA Extraction; 2.2.2 Ф29 Amplification; 2.2.3 Klenow Amplification/Labeling; 2.2.4 Probe and Slide Preparation; 2.2.5 Slide Processing Protocol (for Amino Surfaces); 2.2.6 Hybridization and Slide Washing; 2.2.7 Comments; 2.3 Our Test System and Results; 2.4 Conclusions; References; 3 Sequence-Specific End-Labeling of Oligonucleotides; 3.1 Introduction; 3.2 Probe Design; 3.3 Slide Preparation (Spotting); 3.4 Slide Processing Protocol ( for Aldehyde Surfaces); 3.5 DNA Extraction and PCR Amplification of the Targeted Gene3.6 Shrimp Alkaline Phosphatase Treatment3.7 Labeling; 3.8 Hybridization and Slide Washing; 3.9 Data Analysis; 3.10 Costs; 3.11 Microarray for Detection of Pathogenic Bacteria; References; 4 Non-Cognate Approaches for Pathogen Detection on Microarrays; 4.1 Introduction; 4.2 Non-Cognate Hybridization System; 4.2.1 Concept; 4.2.2 Definition of the Optimal Probe Length; 4.2.3 Virtual Assessment of Array Performances (in Silico Experiments); 4.2.4 Array Manufacturing and Hybridization (Wet-Lab Experiments); 4.2.5 Analysis; 4.3 Perspectives; References; 5 Patterning Techniques for Array Platforms5.1 IntroductionWritten by leading experts in the field as part of an interdisciplinary pan-European research program funded by the EU, the results provided in this booklet provide a unique and comprehensive overview of how microarray technology can be used in safely tracking the most highly dangerous pathogens. A must-have for public health agencies focused on bioterrorism as well as all laboratories working with BSL3 and/or BSL 4 agents.Pathogenic microorganismsDetectionDNA microarraysPathogenic microorganismsDetection.DNA microarrays.579.165Kostic Tanja993881Butaye Patrick993882Schrenzel Jacques993883MiAaPQMiAaPQMiAaPQBOOK9910143129503321Detection of highly dangerous pathogens2275740UNINA00964nam a2200265 i 4500991001821969707536031008s1996 enk 000 0 eng d0856686093b12202186-39ule_instDip.to Scienze dell'Antichitàitaenggrcgrc882.01Euripides229973Bacchae /Euripides ; with an introduction, translation and commentary by Richard SeafordWarminster :Aris & Phillips,1996XI, 270 p. ;22 cmTesto greco a fronteEuripide.BacchaeSeaford, Richard.b1220218602-04-1408-10-03991001821969707536LE007 880.1 Euripides 2001-0312015000069771le007LE015-0309LE015-Vox-E33.03-l- 05150.i1257858708-10-03Bacchae17651UNISALENTOle00708-10-03ma -engenk01