05435nam 2200673Ia 450 991013905510332120200520144314.01-118-63441-11-118-63449-71-118-63456-X(CKB)2560000000102090(EBL)1209634(OCoLC)824890399(SSID)ssj0000886546(PQKBManifestationID)11525249(PQKBTitleCode)TC0000886546(PQKBWorkID)10834742(PQKB)10195119(MiAaPQ)EBC1209634(DLC) 2013002556(Au-PeEL)EBL1209634(CaPaEBR)ebr10716661(CaONFJC)MIL496075(PPN)196492505(EXLCZ)99256000000010209020130116d2013 uy 0engur|n|---|||||txtccrProteomic applications in cancer detection and discovery[electronic resource] /Timothy D. VeenstraHoboken, NJ John Wiley & Sons, Inc.c20131 online resource (320 p.)Includes index.0-471-72406-8 PROTEOMIC APPLICATIONS IN CANCER DETECTION AND DISCOVERY; CONTENTS; PREFACE; ACKNOWLEDGMENTS; 1 SYSTEMS BIOLOGY; 1.1 INTRODUCTION; 1.2 WHAT IS SYSTEMS BIOLOGY?; 1.3 WHAT SYSTEMS DO WE NEED TO STUDY?; 1.3.1 Genomics; 1.3.2 Transcriptomics; 1.3.3 Proteomics; 1.3.4 Metabolomics; 1.4 CANCER IS A SYSTEMS BIOLOGY DISEASE; 1.5 MODELING SYSTEMS BIOLOGY; 1.6 DATA INTEGRATION; 1.6.1 Integrating Transcriptomics and Proteomics; 1.7 CONCLUSIONS; REFERENCES; 2 MASS SPECTROMETRY INCANCER RESEARCH; 2.1 INTRODUCTION; 2.2 MASS SPECTROMETRY: THE TECHNOLOGY DRIVING CANCERPROTEIN BIOMARKER DISCOVERY2.2.1 Ion Sources2.2.2 Electrospray Ionization; 2.2.3 Matrix-Assisted Laser Desorption/Ionization; 2.3 TYPES OF MASS SPECTROMETERS; 2.3.1 Ion-Trap Mass Spectrometer; 2.3.2 Fourier Transform Ion Cyclotron Resonance MS; 2.3.3 Orbitrap Mass Spectrometer; 2.3.4 TOF Mass Spectrometer; 2.3.5 Triple-Quadrupole Mass Spectrometer; 2.3.6 Triple-Quadrupole TOF Mass Spectrometer; 2.4 PROTEIN FRACTIONATION; 2.4.1 Polyacrylamide Gel Electrophoresis; 2.4.2 Liquid Chromatography; 2.5 IMPACT OF MS IN CANCER; 2.5.1 Identification of a Drug Target; 2.6 CONCLUSIONS; REFERENCES; 3 QUANTITATIVE PROTEOMICS3.1 INTRODUCTION3.2 WHAT IS BEING MEASURED IN QUANTITATIVE PROTEOMICS?; 3.3 TWO-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS; 3.4 TWO-DIMENSIONAL DIFFERENCE GEL ELECTROPHORESIS; 3.5 SOLUTION-BASED QUANTITATIVE METHODS; 3.5.1 Stable Isotope Labeling; 3.5.2 Isotope-Coded Affinity Tags; 3.5.3 Isobaric Tag for Relative and Absolute Quantitation; 3.5.4 Stable Isotope Labeling of Amino Acids in Culture; 3.6 NONISOTOPIC SOLUTION-BASED QUANTITATION; 3.6.1 Subtractive Proteomics-Peptide Counting; 3.6.2 Subtractive Proteomics-Peak Intensity; 3.7 CONCLUSIONS; REFERENCES4 PROTEOMIC ANALYSIS OF POSTTRANSLATIONAL MODIFICATIONS4.1 INTRODUCTION; 4.2 PHOSPHORYLATION; 4.2.1 Identification of Phosphorylated Proteins; 4.2.2 Phosphopeptide Mapping; 4.2.3 Collision-Induced Dissociation; 4.2.4 Electron Capture and Electron Transfer Dissociation; 4.2.5 Electron Transfer Dissociation; 4.2.6 Enrichment of Phosphopeptides; 4.2.7 Immunoaffinity Chromatography; 4.2.8 Immobilized Metal Affinity Chromatography; 4.2.9 Metal Oxide Affinity Chromatography; 4.3 GLYCOSYLATION; 4.3.1 Mass Spectrometry Characterization; 4.3.2 Electron Capture and Electron Transfer Dissociation4.3.3 Targeted Identification of Glycoproteins4.3.4 Proteome-Wide Identification of Glycoproteins; 4.4 OTHER POSTTRANSLATIONAL MODIFICATIONS; 4.5 CONCLUSIONS; REFERENCES; 5 CHARACTERIZATION OF PROTEIN COMPLEXES; 5.1 INTRODUCTION; 5.2 METHODS FOR ISOLATING PROTEIN COMPLEXES; 5.2.1 Optimizing Protein Complex Isolation; 5.2.2 Importance of Optimizing Isolation Conditions; 5.2.3 Oligoprecipitation; 5.3 PROTEOME SCREENING USING TANDEM AFFINITY PURIFICATION; 5.4 YEAST TWO-HYBRID SCREENING; 5.5 QUICK LC-MS METHOD TO IDENTIFY SPECIFICALLY BOUND PROTEINS; 5.6 PROTEIN ARRAYS5.7 FLUORESCENCE MICROSCOPY"Bridging the knowledge gap between scientists that develop and apply proteomics technologies and oncologists who focus on understanding the biological basis behind cancer manifestation and progression, Proteomic Applications in Cancer Detection and Discovery provides an up-to-date account of how the multiple facets of proteomics have been applied to cancer. By balancing the treatment of technologies and applications, the book enables analytical scientists and oncologists, post-doctoral researchers, major research or medical centers, cancer researchers, pharmaceutical researchers, chemists, and biologists to better understand both"--Provided by publisher.Biochemical markersCancerGenetic aspectsProteomicsBiochemical markers.CancerGenetic aspects.Proteomics.616.99/4042SCI029000bisacshVeenstra Timothy Daniel1966-981738MiAaPQMiAaPQMiAaPQBOOK9910139055103321Proteomic applications in cancer detection and discovery2240639UNINA02027nas--2200553---450 99000103190020331620240228152936.00074-44410103190USA010103190(ALEPH)000103190USA01010319020020314d--------km-y0itay0103----bafreengNLaka--------Recueil des arrêts, avis consultatifs et ordonnancesReports of judgments, advisory opinions and ordersCour Internationale de JusticeLeydenA.W. 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