02904nam 2200601 450 991013627810332120230621135812.09782889194551 (ebook)(CKB)3710000000586920(SSID)ssj0001680126(PQKBManifestationID)16496184(PQKBTitleCode)TC0001680126(PQKBWorkID)15028316(PQKB)10699518(WaSeSS)IndRDA00057299(oapen)https://directory.doabooks.org/handle/20.500.12854/45325(EXLCZ)99371000000058692020160829d2015 uy |engur|||||||||||txtccrDNA polymerases in biotechnology[electronic resource] /topic editors Andrew F. Gardner and Zvi KelmanFrontiers Media SA2015France :Frontiers Media SA,20151 online resource (146 pages) illustrations, chartsFrontiers Research TopicsBibliographic Level Mode of Issuance: MonographIncludes bibliographical references.DNA polymerases are core tools for molecular biology including PCR, whole genome amplification, DNA sequencing and genotyping. Research has focused on discovery of novel DNA polymerases, characterization of DNA polymerase biochemistry and development of new replication assays. These studies have accelerated DNA polymerase engineering for biotechnology. For example, DNA polymerases have been engineered for increased speed and fidelity in PCR while lowering amplification sequence bias. Inhibitor resistant DNA polymerase variants enable PCR directly from tissue (i.e. blood). Design of DNA polymerases that efficiently incorporate modified nucleotide have been critical for development of next generation DNA sequencing, synthetic biology and other labeling and detection technologies. The Frontiers in Microbiology Research Topic on DNA polymerases in Biotechnology aims to capture current research on DNA polymerases and their use in emerging technologies.Animal BiochemistryHILCCHuman Anatomy & PhysiologyHILCCHealth & Biological SciencesHILCCDNA polymerase evolutionDNA polymerase fidelitymolecular diagnosticsDNA polymerasePCR inhibitorsnext generation sequencingPCRDNA sequencingAnimal BiochemistryHuman Anatomy & PhysiologyHealth & Biological SciencesZvi Kelmanauth1365472Kelman ZviGardner Andrew FPQKBUkMaJRU9910136278103321DNA polymerases in biotechnology3387411UNINA