04756oam 2200601 450 991013486410332120240125224945.03-527-69516-83-527-69518-4(CKB)4340000000003803(EBL)4451920(MiAaPQ)EBC4451920(Au-PeEL)EBL4451920(CaPaEBR)ebr11172364(CaONFJC)MIL909481(OCoLC)945137970(JP-MeL)3000132482(EXLCZ)99434000000000380320160329h20162016 uy 0engur|n|---|||||rdacontentrdamediardacarrierElectrophoresis in practice a guide to methods and applications of DNA and protein separations /Reiner WestermeierFifth edition.Weinheim, Germany :Wiley-VCH Verlag,2016.1 online resource (532 pages)Includes bibliographical references and index3-527-33880-2 Includes bibliographical references at the end of each chapters and index.Title Page; Copyright; Table of Contents; Foreword; Abbreviations, Symbols, Units; Preface; German Version; English Version, Fifth Edition; Part I: Fundamentals; Chapter 1: Electrophoresis; 1.1 General; 1.2 Electrophoresis in Nonrestrictive Gels; 1.3 Electrophoresis in Restrictive Gels; References; Chapter 2: Isotachophoresis; 2.1 Migration with the Same Speed; 2.2 "Ion Train" Separation; 2.3 Zone Sharpening Effect; 2.4 Concentration Regulation Effect; 2.5 Quantitative Analysis; References; Chapter 3: Isoelectric Focusing; 3.1 Principles; 3.2 Gels for IEF; 3.3 Temperature3.4 Controlling the pH Gradient; 3.5 Kinds of pH Gradients; 3.6 Protein Detection in IEF Gels; 3.7 Preparative Isoelectric Focusing; 3.8 Titration Curve Analysis; References; Chapter 4: High-Resolution Two-Dimensional Electrophoresis; 4.1 IEF in Immobilized pH Gradient Strips; 4.2 SDS-PAGE; 4.3 Proteomics; References; Chapter 5: Protein Sample Preparation; 5.1 Protein Quantification Methods; 5.2 Preparation of Native Samples; 5.3 Samples for SDS Electrophoresis; 5.4 Samples for High-Resolution 2D PAGE; References; Chapter 6: Protein Detection; 6.1 Fixation; 6.2 Poststaining Methods6.3 Prelabeling; 6.4 Difference Gel Electrophoresis (DIGE); 6.5 Imaging, Image Analysis, Spot Picking; References; Chapter 7: Blotting; 7.1 Transfer Methods; 7.2 Blotting Membranes; 7.3 Buffers for Electrophoretic Transfers; 7.4 General Staining; 7.5 Blocking; 7.6 Specific Detection; 7.7 Protein Sequencing; 7.8 Transfer Issues; 7.9 Electro-Elution of Proteins from Gels; References; Part II: Equipment and Methods; Chapter 8: Special Laboratory Equipment; Chapter 9: Consumables; Chapter 10: Chemicals; 10.1 Reagents; Method 1: PAGE of Dyes; M1.1 Sample Preparation; M1.2 Stock SolutionsM1.3 Preparing the Casting Cassette; M1.4 Casting Ultra-Thin-Layer Gels; M1.5 Electrophoretic Separation; Method 2: Agarose and Immunoelectrophoresis; M2.1 Sample Preparation; M2.2 Stock Solutions; M2.3 Preparing the Gels; M2.4 Electrophoresis; M2.5 Protein Detection; References; Method 3: Titration Curve Analysis; M3.1 Sample Preparation; M3.2 Stock Solutions; M3.3 Preparing the Blank Gels; M3.4 Titration Curve Analysis; M3.5 Coomassie and Silver Staining; M3.6 Interpreting the Curves; References; Method 4: Native PAGE in Amphoteric-Buffers; M4.1 Sample Preparation; M4.2 Stock SolutionsM4.3 Preparing the Empty Gels; M4.4 Electrophoresis; M4.5 Coomassie and Silver Staining; References; Method 5: Agarose IEF; M5.1 Sample Preparation; M5.2 Preparing the Agarose Gel; M5.3 Isoelectric Focusing; M5.4 Protein Detection; References; Method 6: PAGIEF in Rehydrated Gels; M6.1 Sample Preparation; M6.2 Stock Solutions; M6.3 Preparing the Blank Gels; M6.4 Isoelectric Focusing; M6.5 Coomassie and Silver Staining; M6.6 Perspectives; References; Method 7: Horizontal SDS-PAGE; M7.1 Sample Preparation; M7.2 Prelabeling with Fluorescent Dye; M7.3 Stock Solutions for Gel Preparation; M7.4 Preparing the Casting CassetteElectrophoresisLaboratory manualsDNASeparationLaboratory manualsProteinsSeparationLaboratory manualsElectrophoresisDNASeparationProteinsSeparation543/.4433.4njb/09547.7/04572njb/09Westermeier Reiner626158MiAaPQMiAaPQMiAaPQBOOK9910134864103321Electrophoresis in practice3425722UNINA