01102nam a22002771i 450099100373919970753620040604085313.0040802s1970 it |||||||||||||||||ita b13120220-39ule_instARCHE-107283ExLBiblioteca InterfacoltàitaA.t.i. Arché s.c.r.l. Pandora Sicilia s.r.l.943.086Hitler, Adolf203874Conversazioni di Hitler a tavola :1941-1942 /raccolte al suo quartier generale da Henry Picker ; traduzione di Emmerico GuiscardiMilano :Longanesi,c1970317 p. ;18 cmI libri pocket ;227Hitler, AdolfDiscorsi1941-1942Picker, HenryGuiscardi, Emmerico.b1312022002-04-1405-08-04991003739199707536LE002 Fondo Giudici P 11701LE002G-14896le002C. 1-E0.00-no 00000.i1375573005-08-04Conversazioni di Hitler a tavola308131UNISALENTOle00205-08-04ma -itait 0105237nam 2200637 a 450 991013360190332120240605120810.01-283-02488-897866130248860-470-64936-4(CKB)3400000000000263(EBL)698880(OCoLC)769341494(SSID)ssj0000483684(PQKBManifestationID)11302795(PQKBTitleCode)TC0000483684(PQKBWorkID)10573111(PQKB)10468314(MiAaPQ)EBC698880(PPN)194571343(EXLCZ)99340000000000026320100312d2010 uy 0engur|n|---|||||txtccrCulture of animal cells a manual of basic technique and specialized applications /R. Ian Freshney6th ed.Hoboken, N.J. Wiley-Blackwellc20101 online resource (766 p.)Description based upon print version of record.0-470-64935-6 0-470-52812-5 Includes bibliographical references and index.Culture of Animal Cells; Contents; List of Figures; List of Color Plates; List of Protocols; Preface and Acknowledgements; Abbreviations; 1. Introduction; 1.1. Historical Background; 1.2. Advantages of Tissue Culture; 1.2.1. Control of the Environment; 1.2.2. Characterization and Homogeneity of Samples; 1.2.3. Economy, Scale, and Mechanization; 1.2.4. In vitro Modeling of In vivo Conditions; 1.3. Limitations; 1.3.1. Expertise; 1.3.2. Quantity; 1.3.3. Dedifferentiation and Selection; 1.3.4. Origin of Cells; 1.3.5. Instability; 1.4. Major Differences In vitro; 1.5. Types of Tissue Culture2. Biology of Cultured Cells2.1. The Culture Environment; 2.2. Cell Adhesion; 2.2.1. Cell Adhesion Molecules; 2.2.2. Intercellular Junctions; 2.2.3. Extracellular Matrix; 2.2.4. Cytoskeleton; 2.2.5. Cell Motility; 2.3. Cell Proliferation; 2.3.1. Cell Cycle; 2.3.2. Control of Cell Proliferation; 2.4. Differentiation; 2.4.1. Maintenance of Differentiation; 2.4.2. Dedifferentiation; 2.5. Cell Signaling; 2.6. Energy Metabolism; 2.7. Origin of Cultured Cells; 2.7.1. Initiation of the Culture; 2.7.2. Evolution of Cell Lines; 2.7.3. Senescence2.7.4. Transformation and the Development of Continuous Cell Lines3. Laboratory Design, Layout, and Equipment; 3.1. Layout, Furnishing, and Services; 3.1.1. Requirements; 3.1.2. Services; 3.1.3. Ventilation; 3.2. Layout; 3.2.1. Sterile Handling Area; 3.2.2. Laminar Flow; 3.2.3. Service Bench; 3.2.4. Quarantine and Containment; 3.2.5. Incubation; 3.2.6. Preparation Area; 3.2.7. Storage; 4. Equipment and Materials; 4.1. Requirements of a Tissue Culture Laboratory; 4.2. Aseptic Area; 4.2.1. Laminar-Flow Hood; 4.2.2. Service Carts; 4.2.3. Sterile Liquid Handling-Pipetting and Dispensing4.2.4. Inverted Microscope4.2.5. CCD Camera and Monitor; 4.2.6. Dissecting Microscope; 4.2.7. Centrifuge; 4.2.8. Cell Counting; 4.3. Incubation and Culture; 4.3.1. Incubator; 4.3.2. Humid CO2 Incubator; 4.3.3. Temperature Recorder; 4.3.4. Roller Racks; 4.3.5. Magnetic Stirrer; 4.3.6. Culture Vessels; 4.4. Preparation and Sterilization; 4.4.1. Washup; 4.4.2. Preparation of Media and Reagents; 4.4.3. Sterilization; 4.5. Storage; 4.5.1. Consumables; 4.5.2. Refrigerators and Freezers; 4.5.3. Cryostorage Containers; 4.5.4. Controlled-Rate Freezer; 4.6. Supplementary Laboratory Equipment4.6.1. Computers and Networks4.6.2. Upright Microscope; 4.6.3. Low-Temperature Freezer; 4.6.4. Confocal Microscope; 4.6.5. PCR Thermal Cycler; 4.7. Specialized Equipment; 4.7.1. Microinjection Facilities; 4.7.2. Colony Counter; 4.7.3. Centrifugal Elutriator; 4.7.4. Flow Cytometer; 5. Aseptic Technique; 5.1. Objectives of Aseptic Technique; 5.1.1. Risk of Contamination; 5.1.2. Maintaining Sterility; 5.2. Elements of Aseptic Environment; 5.2.1. Laminar Flow; 5.2.2. Quiet Area; 5.2.3. Work Surface; 5.2.4. Personal Hygiene; 5.2.5. Reagents and Media; 5.2.6. Cultures; 5.3. Sterile Handling5.3.1. SwabbingThis is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Rigorously revised, it features updates on specialized techniques in stem cell research and tissue engineering; updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer; new sections on vitrification and Organotypic Culture, and new chapters on epithelial, mesenchymal, neurectodermal, and hematopoietic cells; germs cells/stemcells/amniocytes; and non-mammalian/avian cells. It is written for graduate students, research and clinical scientists, and technicians and laTissue cultureLaboratory manualsCell cultureLaboratory manualsTissue cultureCell culture571.6/381571.6381Freshney R. Ian66884MiAaPQMiAaPQMiAaPQBOOK9910133601903321Culture of animal cells377367UNINA