05104nam 2200625 450 991013228180332120230807213818.03-527-68798-X3-527-68797-13-527-68799-8(CKB)3710000000361509(EBL)1896080(SSID)ssj0001471626(PQKBManifestationID)11820214(PQKBTitleCode)TC0001471626(PQKBWorkID)11425388(PQKB)11738549(MiAaPQ)EBC1896080(Au-PeEL)EBL1896080(CaPaEBR)ebr11025877(CaONFJC)MIL769810(OCoLC)904405254(EXLCZ)99371000000036150920150317h20152015 uy 0engur|n|---|||||txtccrModern techniques for pathogen detection /edited by Jürgen Popp and Michael BauerWeinheim, Germany :Wiley Blackwell,2015.©20151 online resource (353 p.)Description based upon print version of record.3-527-33516-1 Includes bibliographical references at the end of each chapters and index.Cover; Contents; Preface; List of Contributors; Chapter 1 Unmet Medical Needs in Life-Threatening Infections - Caring for the Critically Ill; 1.1 Life Threatening Infections and Sepsis - Defining the Problem; 1.2 Sepsis as a ""Hidden Healthcare Disaster""; 1.3 Microorganisms and Types of Infection Triggering Sepsis; 1.4 Emerging Problems Related to Resistance in Bacterial Infections; 1.5 The Role of Fungi and Viruses; 1.6 The Need for New Approaches in Diagnostics of Life-Threatening Infection and Sepsis1.7 Rapid and Sensitive Culture-Independent Strategies to Identify Blood Stream Infection1.8 Beyond Infection - Profiling the Immune Response of the Septic Host; 1.9 Host Factors Contributing to Pathogenesis of Sepsis; References; Chapter 2 Identification Methods - An Overview; 2.1 Taxonomy of Pathogenic Organisms; 2.2 Microscopic Methods; 2.2.1 Stains; 2.2.2 Autofluorescence; 2.2.3 Immunofluorescence; 2.2.4 In Situ Hybridization; 2.2.5 Dark-Field Microscopy; 2.3 Culture-Based Methods; 2.3.1 Blood Culture; 2.3.2 Automated Differentiation Systems; 2.3.3 API System; 2.3.4 Selective Agar2.3.5 Susceptibility Testing2.3.6 Other Methods; 2.4 Nucleic Acid-Based Techniques; 2.4.1 PCR; 2.4.1.1 Real-Time PCR; 2.4.1.2 Multiplex-PCR; 2.4.1.3 RAPD and rep-PCR; 2.4.1.4 Microarrays; 2.4.1.5 RFLP; 2.4.2 Sequencing; 2.4.2.1 Ribosomal RNA Genes; 2.4.2.2 MLST; 2.4.2.3 NG Sequencing; 2.5 Serology; 2.5.1 Antigen-Antibody-Based Methods; 2.5.1.1 Agglutination; 2.5.1.2 Immunodiffusion; 2.5.1.3 Quantitative Immunoassays; 2.5.2 Automated Immunoassays; 2.5.3 Applications of Serological Test; 2.5.3.1 Types of Human Antibodies and What They Indicate?2.5.3.2 Validity of Antibodies against Opportunistic Species2.5.3.3 Specificity of Fungal Antigens; 2.5.3.4 Specific Urine Antigen Tests; 2.5.4 Biomarkers; 2.6 Conclusions and Perspectives; References; Chapter 3 Nucleic Acid Amplification Techniques; 3.1 Introduction; 3.2 The Basic PCR Protocol; 3.3 Primer Design; 3.3.1 Specific versus Broad-Range Primers and the Fate of False Targeting; 3.4 Modified End-Point PCR Protocols; 3.5 Non-PCR NAT: Isothermal Amplification Protocols; 3.6 Quantitative PCR; 3.6.1 Quantification in qPCR; 3.6.2 Melting Curve Analysis (MCA)3.7 Controls, Probing, and General Aspects of Result Interpretation3.7.1 Strategies in Amplicon Verification; 3.7.1.1 (DNA) Microarray; 3.7.1.2 Flow Cytometry; 3.7.1.3 Mass Spectrometry; 3.7.2 PCR Inhibitors; 3.8 Preanalytics; 3.8.1 Sample Volume; 3.8.2 Cell Lysis; 3.8.3 Nucleic Acid Isolation and Preparation; 3.9 Fields of PCR Application; 3.9.1 Sequencing; 3.9.1.1 Pyrosequencing; 3.9.2 Typing and Epidemiology; 3.9.3 Pathogen Detection in Complex Clinical Samples; 3.9.4 General Aspects of Result Interpretation; 3.9.4.1 Sensitivity and Specificity; 3.9.4.2 NAT versus Culture3.9.4.3 The Fate of Antibiotic Resistances DetectionThis outstanding overview sets a new standard for a methods book on pathogen detection. The first chapter provides an outline of currently used routine methods, including their background, strengths and weaknesses, as well as comparing them to newer methods. The following chapters then cover novel methods already in wide use and which are still more experimental for routine purposes.<br>An invaluable resource for all medical laboratories and clinical institutions dealing with infectious diseases.<br>Pathogenic microorganismsDetectionPathogenic microorganismsDetection.616.9Popp JürgenBauer MichaelMiAaPQMiAaPQMiAaPQBOOK9910132281803321Modern techniques for pathogen detection2118806UNINA