05503nam 2200733 450 991013218680332120230803203826.01-118-33611-91-118-33614-31-118-33613-5(CKB)3710000000204404(EBL)1753758(OCoLC)885019005(SSID)ssj0001288363(PQKBManifestationID)12443341(PQKBTitleCode)TC0001288363(PQKBWorkID)11294064(PQKB)11390816(PQKBManifestationID)16046358(PQKB)20392911(OCoLC)889316924(MiAaPQ)EBC1753758(DLC) 2014022003(Au-PeEL)EBL1753758(CaPaEBR)ebr10902335(CaONFJC)MIL632093(OCoLC)881064963(EXLCZ)99371000000020440420140815h20142014 uy 0engurcnu||||||||txtccrBiofouling methods /edited by Sergey Dobretsov, Jeremy C. Thomason, David N. WilliamsFirst edition.West Sussex, England :John Wiley & Sons, Ltd.,2014.©20141 online resource (411 p.)Includes index.0-470-65985-8 Includes bibliographical references and index.Biofouling Methods; Copyright; Contents; List of contributors; Introduction; Guide to methods; Part I Methods for Microfouling ; Chapter 1 Microscopy of biofilms; Section 1: Traditional light and epifluorescent microscopy; 1.1 Introduction; 1.2 Determination of bacterial abundance; 1.3 Catalyzed reporter deposition fluorescent in situ hybridization (CARD-FISH); 1.4 Suggestions, with examples, for data analysis and presentation; Acknowledgements; References; Chapter 2 Traditional and bulk methods for biofilms; Section 1: Traditional microbiological methods; 2.1 Introduction2.2 Enrichment culture, isolation of microbes 2.3 Counting methods; 2.4 Troubleshooting hints and tips; References; Section 2 Bulk methods; 2.5 Introduction; 2.6 Measurement of biofilm thickness; 2.7 Biofilm dry weight determination; 2.8 Biofilm ATP content; 2.9 Troubleshooting hints and tips; Acknowledgements; References; Chapter 3 Biocide testing against microbes; Section 1: Testing biocides in solution: flow cytometry for planktonic stages; 3.1 Introduction; 3.2 Method introductions; 3.3 Pros and cons; 3.4 Materials and equipment; 3.5 Methods; 3.6 Troubleshooting hints and tips3.7 Suggestions References; Section 2 Biocide testing using single and multispecies biofilms; 3.8 Introduction; 3.9 Questions to answer when applying biocides; 3.10 Laboratory methods for testing biocide effect; 3.11 Field methods for testing biocide effect; 3.12 Troubleshooting hints and tips; Acknowledgements; References; Chapter 4 Molecular methods for biofilms; Section 1: Isolation of nucleic acids; 4.1 Introduction; 4.2 Materials; 4.3 Isolation of DNA from a biofilm; 4.4 Troubleshooting hints and tips; References; Section 2 PCR and DNA sequencing4.5 PCR and DNA sequencing: General introduction 4.6 PCR; 4.7 Microbial marker genes - 16S; 4.8 DNA sequencing; 4.9 454 16S amplicon pyrotag sequencing; 4.10 Protocol 1: DNA extraction using the Qiagen DNeasy Plant Mini Kit; 4.11 Protocol 2: Full-length 16S PCR using the Qiagen Multiplex Kit; 4.12 Protocol 3: Analysis of full-length 16S genes; 4.13 Protocol 4: 16S amplicon PCR for 454 sequencing using the Qiagen Multiplex Kit; 4.14 Protocol 5: Trimming and filtering of 454 16S pyrotag sequencing; 4.15 Protocol 6: Taxon-based analyses; 4.16 Protocol 7: Phylogeny-based analyses; ReferencesSection 3 Community comparison by genetic fingerprinting techniques 4.17 Introduction; 4.18 History and principles of the methods; 4.19 Advantages and limitations of fingerprinting techniques; 4.20 Materials and equipment; 4.21 Suggestions for data analysis and presentation; 4.22 Troubleshooting hints and tips; Acknowledgements; References; Section 4 Metagenomics; 4.23 Introduction and brief summary of methods; 4.24 Overview of metagenomics methods; 4.25 Method introduction; 4.26 Overview of DNA handling for BAC library construction; 4.27 BAC and Fosmid library construction4.28 Library handling, archiving, and databasingBiofouling Methods provides a "cook book" for both established workers and those new to the field. The methods included in this important new book range from tried and tested techniques to those at the cutting edge, encompassing the full diversity of this multidisciplinary field. The book covers methods for microbial and macrofouling, coatings and biocides, and ranges from methods for fundamental studies to methods relevant for industrial applications. There is an emphasis on answering questions and each chapter provides technical methods and problem-solving hints and tips. Bringing togetheFoulingFouling organismsFouling.Fouling organisms.628.9/6 Dobretsov SergeyThomason Jeremy C.Williams David N.MiAaPQMiAaPQMiAaPQBOOK9910132186803321Biofouling methods2259738UNINA