01755nlm0 22004691i 450 9900092626504033219783642045769000926265FED01000926265(Aleph)000926265FED0100092626520100926d2009----km-y0itay50------baengDEdrnn-008mamaaTraffic Management and Traffic Engineering for the Future InternetRisorsa elettronicaFirst Euro-NF Workshop, FITraMEn 2008, Porto, Portugal, December 11-12, Revised Selected Papersedited by Rui Valadas, Paulo SalvadorBerlin ; HeidelbergSpringer2009Lecture Notes in Computer Science0302-97435464Documento elettronicoTestoFormato html, pdfSalvador,PauloValadas,RuiITUNINAREICATUNIMARCFull text per gli utenti Federico IIhttp://dx.doi.org/10.1007/978-3-642-04576-9EB990009262650403321Computer Communication NetworksComputer Communication NetworksComputer scienceComputer ScienceComputer system performanceInformation systemsInformation SystemsInformation Systems and Communication ServiceInformation Systems Applications (incl.Internet)Management of Computing and Information SystemsMultimedia Information SystemsMultimedia systemsSystem Performance and EvaluationTraffic Management and Traffic Engineering for the Future Internet773689UNINA05427nam 2200661Ia 450 991046215800332120200520144314.03-527-66862-41-283-64410-X3-527-66861-6(CKB)2670000000254018(EBL)1031847(OCoLC)811963710(SSID)ssj0000722590(PQKBManifestationID)12313404(PQKBTitleCode)TC0000722590(PQKBWorkID)10698829(PQKB)10872894(MiAaPQ)EBC1031847(Au-PeEL)EBL1031847(CaPaEBR)ebr10605313(CaONFJC)MIL395660(EXLCZ)99267000000025401820120813d2012 uy 0engur|n|---|||||txtccrEpigenetic regulation and epigenomics[electronic resource] /edited by Robert A. MeyersWeinheim Wiley-Blackwellc20121 online resource (1239 p.)Advances in molecular biology and medicineDescription based upon print version of record.3-527-32682-0 Includes bibliographical references and index.Epigenetic Regulation and Epigenomics; Contents; Preface and Commentary; List of Contributors; Part I Analytical Methods; 1 RNA Methodologies; 1 Introduction; 2 Subpopulations of RNA; 3 Goals in the Purification of RNA; 4 Methods of Cellular Disruption and RNA Recovery; 5 Inhibition of Ribonuclease Activity; 6 Methods for the Analysis of RNA; 7 Summary; References; 2 All Things ChIP: ChIP-Chip, ChIP-Seq, ChIP-PCR; 1 Introduction; 2 Protein-DNA Binding; 3 ChIP Protocol; 4 ChIP-PCR, ChIP-Chip, or ChIP-Seq. Which Should be Chosen?; 5 Experimental Considerations; 6 Calculating IP Enrichment7 Special Analysis Considerations8 Conclusions; References; 3 Methods for DNA Methylation Analysis; 1 Introduction; 2 Methods of DNA Methylation Analysis; 3 Concluding Remarks; Acknowledgments; References; 4 DNA Methylation Analysis by MALDI Mass Spectrometry; Abbreviations; 1 Introduction to DNA Methylation; 2 Epigenetics and Disease; 3 DNA Methylation Content Analysis by Mass Spectrometry; 4 Specific DNA Methylation Analysis; 5 Introduction to MALDI; 6 Problems of MALDI Analysis of; 7 DNA Sequencing with MALDI Mass Spectrometry Readout8 Primer Extension for the Interrogation of Specific Positions9 Quantification; 10 Automation of Sample Preparation, Data Accumulation, and Analysis; 11 Positioning of MALDI MS for DNA Methylation Analysis; 12 Applications of DNA Methylation Analysis by Mass Spectrometry; 13 DNA Methylation Analysis for Tumor Classification; 14 DNA Methylation Analysis for Prognosis and Response to Treatment; 15 Prenatal Diagnosis; 16 Conclusions; References; 5 Tag Sequencing; 1 Library Construction; 2 Advanced Sequencing Technologies; 3 Data Analysis and Bioinformatics; 4 Applications of Tag Sequencing5 Clinical Applications of Tag Sequencing6 Future Perspectives; Acknowledgments; References; Part II Basic Molecular Mechanisms; 6 Heterochromatin and Euchromatin - Organization, Boundaries and Gene Regulation; 1 Properties and Functions of Heterochromatin; 2 Euchromatin Formation; 3 Boundaries between Heterochromatin and Euchromatin; 4 Insulating against Active Chromatin; Acknowledgments; References; 7 Regulation of Gene Expression; 1 Introduction; 2 Regulation of Gene Expression in Prokaryotes; 3 Regulation of Gene Expression in Eukaryotes; 4 RNA Splicing5 Role of microRNAs (miRNAs) in the Regulation of Gene Expression6 Chromatin Structure and the Control of Gene Expression; 7 Epigenetic Control of Gene Expression; 8 Gene Regulation by Hormonal Action; 9 Post-Transcriptional Regulation of mRNA; 10 Transport of Processed mRNA to the Cytoplasm; 11 Regulation of Gene Expression at the Level of Translation; Acknowledgments; References; 8 Molecular Genetics of Genomic Imprinting; 1 Genomic Imprinting in Mammals; 2 Imprinted Genes; 3 Molecular Mechanisms; 4 Imprinting and Disease; 5 Human Reproduction; 6 Evolution of Imprinting; References9 Nuclear Transfer for Cloning AnimalsEpigenetics is a term in biology referring to heritable traits that do not involve changes in the underlying DNA sequence of the organism. Epigenetic traits exist on top of or in addition to the traditional molecular basis for inheritance. The ""epigenome"" is a parallel to the word ""genome,"" and refers to the overall epigenetic state of a cell. Cancer and stem cell research have gradually focused attention on these genome modifications. The molecular basis of epigenetics involves modifications to DNA and the chromatin proteins that associate with it. Methylation, for example, can silence a Current Topics from the Encyclopedia of Molecular Cell Biology and Molecular MedicineGenetic regulationEpigenesisElectronic books.Genetic regulation.Epigenesis.572.865Meyers Robert A(Robert Allen),1936-8008MiAaPQMiAaPQMiAaPQBOOK9910462158003321Epigenetic regulation and epigenomics1918396UNINA