03387nam 2200385 450 991064594480332120230510162522.0(CKB)5860000000285478(NjHacI)995860000000285478(EXLCZ)99586000000028547820230510d2022 uy 0engur|||||||||||txtrdacontentcrdamediacrrdacarrierIntermediate Filament Mechanics Across Scales From Single Filaments to Single Interactions and Networks in Cells /Anna Veronika Schepers[Place of publication not identified] :Universitätsverlag Göttingen,2022.1 online resource (iv, 234 pages) illustrationsGöttingen Series in BiophysicsThe mechanical properties of cells are largely determined by the cytoskeleton. The cytoskeleton is an intricate and complex structure formed by protein filaments, motor proteins, and crosslinkers. The three main types of protein filaments are microtubules, actin filaments, and intermediate filaments ( IFs ). Whereas the proteins that form microtubules and actin filaments are exceptionally conserved throughout cell types and organisms, the family of IFs is diverse. For example, the IF protein vimentin is expressed in relatively motile fibroblasts, and keratin IFs are found in epithelial cells. This variety of IF proteins might therefore be linked to the various mechanical properties of different cell types. In the scope of this thesis, I combine studies of IF mechanics on different time scales and in systems of increasing complexity, from single filaments to networks in cells. This multiscale approach allows for the simplification necessary to interpret observations while adding increasing physiological context in subsequent experiments. We especially focus on the tunability of the IF mechanics by environmental cues in these increasingly complex systems. In a series of experiments, including single filament elongation studies, single filament stretching measurements with optical tweezers, filament-filament interaction measurements with four optical tweezers, microrheology, and isotropic cell stretching, we characterize how electrostatic (pH and ion concentration) and hydrophobic interactions (detergent) provide various mechanisms by which the mechanics of the IF cytoskeleton can be tuned. These studies reveal how small changes, such as charge shifts, influence IF mechanics on multiple scales. In combination with simulations, we determine the mechanisms by which charge shifts alter single vimentin filament mechanics and we extract energy landscapes for interactions between single filaments. Such insights will provide a deeper understanding of the mechanisms by which cells can maintain their integrity and adapt to the mechanical requirements set by their environment.Cytoplasmic filamentsOptical tweezersCellsMechanical propertiesCytoplasmic filaments.Optical tweezers.CellsMechanical properties.571.6Schepers Anna Veronika1348122NjHacINjHaclBOOK9910645944803321Intermediate filament mechanics across scales3085190UNINA01995nam2 22003973i 450 TO0036072320231121125837.08802035660IT826901 19960416d1981 ||||0itac50 baitaitz01i xxxe z01nVol. 3di Carl Ludwig von Hallera cura di Mario SanciprianoTorinoUnione tipografico-editrice torinese1981678 p., [6] carte di tav.ill.24 cmTraduzione di Marilla Boffito, Mario Sancipriano e Piero Sancipriano001RMG00163512001 ˜La œrestaurazione della scienza politicadi Carl Ludwig von Hallera cura di Mario Sancipriano3Sancipriano, MarioCFIV074994340Boffito, MarillaRAVV073546730Sancipriano, PieroSBLV015450730ITIT-0119960416IT-RM028 IT-RM0289 IT-RM0542 IT-RM0418 IT-FR0084 IT-FR0017 IT-RM0574 Biblioteca Universitaria AlessandrinaRM028 Biblioteca Statale A. BaldiniRM0289 BIBLIOTECA DEL MINISTERO DEGLI AFFARI ESTERIRM0542 BIBLIOTECA ACCADEMIA NAZ. DEI LINCEI E CORSINIANARM0418 Biblioteca Del Monumento Nazionale Di MontecassinoFR0084 Biblioteca umanistica Giorgio ApreaFR0017 Biblioteca del Consiglio nazionale dell'economia e del lavoroRM0574 TO00360723Biblioteca umanistica Giorgio Aprea 52S.L. 320 UTET Hal 3 52MAG0000155215 VMB RS C 2012092520120926 01 04 09 10 25 52 61Parole di un credente come le scrisse l'abate F. de Lamennais quando era un credente2860897UNICAS