1.

Record Nr.

UNISA996466198603316

Titolo

Internet Science [[electronic resource] ] : INSCI 2018 International Workshops, St. Petersburg, Russia, October 24–26, 2018, Revised Selected Papers / / edited by Svetlana S. Bodrunova, Olessia Koltsova, Asbjørn Følstad, Harry Halpin, Polina Kolozaridi, Leonid Yuldashev, Anna Smoliarova, Heiko Niedermayer

Pubbl/distr/stampa

Cham : , : Springer International Publishing : , : Imprint : Springer, , 2019

ISBN

3-030-17705-X

Edizione

[1st ed. 2019.]

Descrizione fisica

1 online resource (XI, 283 p. 88 illus., 29 illus. in color.)

Collana

Information Systems and Applications, incl. Internet/Web, and HCI ; ; 11551

Disciplina

004.678

Soggetti

Application software

User interfaces (Computer systems)

Computer organization

Information Systems Applications (incl. Internet)

User Interfaces and Human Computer Interaction

Computer Appl. in Social and Behavioral Sciences

Computer Systems Organization and Communication Networks

Lingua di pubblicazione

Inglese

Formato

Materiale a stampa

Livello bibliografico

Monografia

Note generali

Includes index.

Nota di contenuto

Detecting Social Problems in Online Content -- CONVERSATIONS 2018 - 2nd International Workshop on Chatbot Research -- The Future of Decentralized Governance Workshop -- Internet as an Issue: an International Workshop on Government and Media Narratives.

Sommario/riassunto

This book constitutes the refereed post-conference proceedings of 4 workshops, held at the 5th International Conference on Internet Science, St. Petersburg, Russia, in October 2018: Workshop 1 : Detecting Social Problems in Online Content, Workshop 2: CONVERSATIONS, Workshop 3: The Future of Decentralized Governance: A Workshop on Encryption, Blockchains, and Personal Data, and Workshop 4: Internet as an issue: An international workshop on government and media narratives. The 20 full papers presented



together with 4 short papers were carefully reviewed and selected from 38 submissions. The contributions of the Workshop 1: Detecting Social Problems in Online Content has united Russian scholars who work upon Russian-language datasets. Workshop 2: CONVERSATIONS: An international workshop on chatbot research and design regularly discusses the novel issues in their research and production area. Workshop 3: The Future of Decentralized Governance: A Workshop on Encryption, Blockchains, and Personal Data. At this workshop scholars and industry representatives from France, the Netherlands, the UK, and Russia have discussed distributed governance technologies based on blockchain and other privacy-protecting technologies. Workshop 4: Internet as an issue: An international workshop on government and media narratives took a rare approach and regarded Internet as a focus for public discussion.

2.

Record Nr.

UNINA9910254147703321

Autore

Terasaka Naohiro

Titolo

Applications of Aminoacylation Ribozymes That Recognize the 3′-end of tRNA / / by Naohiro Terasaka

Pubbl/distr/stampa

Tokyo : , : Springer Japan : , : Imprint : Springer, , 2017

ISBN

9784431565154

9784431565178 (e-book)

Edizione

[1st ed. 2017.]

Descrizione fisica

1 online resource (XII, 89 p. 38 illus., 28 illus. in color.)

Collana

Springer Theses, Recognizing Outstanding Ph.D. Research, , 2190-5053

Disciplina

540

Soggetti

Bioorganic chemistry

Catalysis

Nucleic acids

Biotechnology

Bioorganic Chemistry

Nucleic Acid Chemistry

Lingua di pubblicazione

Inglese

Formato

Materiale a stampa

Livello bibliografico

Monografia

Note generali

"Doctoral Thesis accepted by the University of Tokyo, Tokyo, Japan."

Nota di bibliografia

Includes bibliographical references.



Nota di contenuto

General introduction -- Discovery of human microRNA precursor binding to folic acid by small RNA transcriptomic SELEX -- Orthogonal ribosome-tRNAs pair by engineering of peptidyl transferase center -- General conclusion.

Sommario/riassunto

In this thesis, applications of aminoacylation ribozymes named flexizymes are described. Flexizymes have the following unique characteristics: (i) substrate RNA is recognized by two consecutive base pairs between the 3'-end of substrate RNA and the 3'-end of the flexizyme; (ii) these base pairs can be substituted with other base pairs; and (iii) various activated amino acids can be used as substrates including both canonical and noncanonical amino acids. This flexible aminoacylation of RNAs by flexizymes was used to label endogenous tRNAs to be removed, and in vitro selection using the tRNA-depleted library enabled the discovery of the novel interaction between the microRNA precursor and metabolites. Flexizymes are also used to prepare various aminoacyl-tRNAs bearing mutations at the 3'-end to engineer the translation machinery and to develop the orthogonal translation machinery. The first part of the research demonstrated that SELEX is appropriate for discovering the interaction between small RNA and ligands, and suggested that more RNA motif binding to small molecules exists in small RNAs. The second part opened a door to new opportunities for in vitro synthetic biology involving the engineering of the genetic codes and translation machineries. This research also indicated the great potential of aminoacylation by flexizymes to be applied in various fields of RNA research, which is beneficial for RNA researchers.