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Record Nr. |
UNINA9910143983303321 |
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Titolo |
High-throughput screening in drug discovery [[e-book] /] / edited by Jörg Hüser |
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Pubbl/distr/stampa |
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Weinheim, : Wiley-VCH |
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[Chichester, : John Wiley, distributor], c2006 |
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ISBN |
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1-280-72366-1 |
9786610723669 |
3-527-60932-6 |
3-527-60936-9 |
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Descrizione fisica |
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1 online resource (371 p.) |
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Collana |
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Methods and principles in medicinal chemistry ; ; v. 35 |
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Classificazione |
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Altri autori (Persone) |
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Disciplina |
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Soggetti |
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High throughput screening (Drug development) |
Pharmaceutical chemistry |
Electronic books. |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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High-Throughput Screening in Drug Discovery; Foreword; List of Contents; Preface; List of Contributors; Part I Concept of Screening; 1 Chemical Genetics: Use of High-throughput Screening to Identify Small-molecule Modulators of Proteins Involved in Cellular Pathways with the Aim of Uncovering Protein Function; 1.1 Introduction; 1.2 Classical and Chemical Genetics; 1.2.1 Forward and Reverse Screens; 1.3 Identifying Bioactive Molecules; 1.4 Target Identification; 1.4.1 Hypothesis-driven Target Identification; 1.4.2 Affinity-based Target Identification |
1.4.3 Genomic Methods of Target Identification1.4.4 Proteomic Methods; 1.5 Discovery for Basic Research Versus Pharmacotherapy Goals; 1.6 Chemical Genetic Screens in the Academic Setting; 1.7 Conclusions; 2 High-throughput Screening for Targeted Lead Discovery; 2.1 Chemical Libraries for High-throughput Screening; 2.2 Properties of Lead Structures; 2.3 Challenges to High-throughput Screening; 2.4 Assay Technologies for High-throughput Screening; 2.5 |
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Laboratory Automation; 2.6 From Target Selection to Confirmed Hits - the HTS Workflow and its Vocabulary |
2.7 Separating Specific Modulators from Off-Target Effects2.8 Data Analysis and Screening Results; 2.9 Conclusions; Part II Automation Technologies; 3 Tools and Technologies that Facilitate Automated Screening; 3.1 Introduction - the Necessity to Automate; 3.1.1 Compound Libraries; 3.1.2 Targets and Data Points; 3.1.3 Main Issues Facing HTS Groups Today; 3.1.4 Benefits of Miniaturization; 3.1.5 Benefits of Automated HTS; 3.1.6 Screening Strategies; 3.1.7 Ultra HTS (UHTS); 3.2 Sample Carriers; 3.2.1 A Brief History of the Microplate; 3.2.2 Microplate Usage Today; 3.2.3 Microplate Arrays |
3.2.4 Non-microplate Alternatives3.2.4.1 Labchips; 3.2.4.2 LabCDs; 3.2.4.3 LabBrick; 3.2.4.4 Arrayed Compound Screening; 3.3 Liquid Handling Tools; 3.3.1 Main Microplate Dispense Mechanisms; 3.3.1.1 Pin Tools; 3.3.1.2 Air and Positive Displacement; 3.3.1.3 Peristaltic; 3.3.1.4 Solenoid-syringe; 3.3.1.5 Solenoid-pressure bottle; 3.3.1.6 Capillary Sipper; 3.3.1.7 Piezoelectric; 3.3.1.8 Acoustic Transducer; 3.3.2 HTS Liquid Handling Applications and Dispensing Technologies Used; 3.3.2.1 Bulk Reagent and Cell Addition; 3.3.2.2 Compound Reformatting and Nanoliter Dispensing |
3.3.2.3 Cherry Picking and Serial Dilution3.3.2.4 Microplate Washing; 3.4 Detection Technologies; 3.4.1 Main Detection Modalities Used in HTS; 3.4.2 Plate Readers; 3.4.3 Plate Imagers; 3.4.3.1 Macro-imaging; 3.4.3.2 Micro-imaging; 3.4.4 Dispense and Read Devices; 3.4.5 Other Detection Technologies; 3.4.6 Automation of Detection Technologies; 3.4.7 Potential Sources of Reading Error; 3.5 Laboratory Robotics; 3.5.1 Traditional Workstations; 3.5.2 Robotic Sample Processors; 3.5.3 Plate Storage Devices; 3.5.4 Plate Moving Devices; 3.5.5 Fully Integrated Robotic Systems; 3.5.6 Turnkey Workstations |
3.5.7 Automated Cell Culture Systems |
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Sommario/riassunto |
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Backed by leading authorities, this is a professional guide to successful compound screening in pharmaceutical research and chemical biology, including the chemoinformatic tools needed for correct data evaluation. Chapter authors from leading pharmaceutical companies as well as from Harvard University discuss such factors as chemical genetics, binding, cell-based and biochemical assays, the efficient use of compound libraries and data mining using cell-based assay results.For both academics and professionals in the pharma and biotech industries working on small molecule screening. |
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2. |
Record Nr. |
UNISA996386781503316 |
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Autore |
Younge Richard |
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Titolo |
A counterpoyson, or, Soverain antidote against all griefe [[electronic resource] ] : as also, The benefit of affliction, and how to husband it so, that the weakest Christian (with blessing from above) may be able to support himself in his most miserable exigents : together with The victory of patience, extracted out of the choisest authors, ancient and moderne, both holy and humane : necessary to be read of all that any way suffer tribulation / / by R. Young |
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Pubbl/distr/stampa |
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London, : Printed by J.B. and S.B., and are to be sold by Philip Nevill ..., 1641 |
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Edizione |
[The second edition /] |
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Descrizione fisica |
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Soggetti |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Reproduction of original in Union Theological Seminary Library, New York. |
Attributed to Richard Younge. cf. NUC pre-1956. |
Running title: The victory of patience. |
Index: p. [1]-[12] |
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Nota di contenuto |
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The benefit of affliction. -- Three prayers for a family. -- The state of a Christian. |
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Sommario/riassunto |
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