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Record Nr. |
UNISA996218619003316 |
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Autore |
Albani Jihad René <1956-> |
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Titolo |
Principles and applications of fluorescence spectroscopy [[electronic resource] /] / Jihad René Albani |
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Pubbl/distr/stampa |
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Oxford ; ; Ames, Iowa, : Blackwell Science, c2007 |
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ISBN |
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1-281-31900-7 |
9786611319007 |
0-470-69205-7 |
0-470-69133-6 |
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Edizione |
[1st ed.] |
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Descrizione fisica |
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1 online resource (270 p.) |
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Disciplina |
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Soggetti |
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Fluorescence spectroscopy |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Principles and Applications of Fluorescence Spectroscopy; Contents; 1 Absorption Spectroscopy Theory; 1.1 Introduction; 1.2 Characteristics of an Absorption Spectrum; 1.3 Beer-Lambert-Bouguer Law; 1.4 Effect of the Environment on Absorption Spectra; References; 2 Determination of the Calcofluor White Molar Extinction Coefficient Value in the Absence and Presence of a1-Acid Glycoprotein; 2.1 Introduction; 2.2 Biological Material Used; 2.2.1 Calcofluor White; 2.2.2 a1-Acid glycoprotein; 2.3 Experiments; 2.3.1 Absorption spectrum of Calcofluor free in PBS buffer |
2.3.2 Determination of e. value of Calcofluor White free in PBS buffer 2.3.3 Determination of Calcofluor White e. value in the presence of a1-acid glycoprotein; 2.4 Solution; References; 3 Determination of Kinetic Parameters of Lactate Dehydrogenase; 3.1 Objective of the Experiment; 3.2 Absorption Spectrum of NADH; 3.3 Absorption Spectrum of LDH; 3.4 Enzymatic Activity of LDH; 3.5 Kinetic Parameters; 3.6 Data and Results; 3.6.1 Determination of enzyme activity; 3.6.2 Determination of kinetic parameters; 3.7 Introduction to Kinetics and the Michaelis-Menten Equation; 3.7.1 Definitions |
3.7.2 Reaction rates References; 4 Hydrolysis of p-Nitrophenyl-B-D-Galactoside with B-Galactosidase from E. coli; 4.1 Introduction; 4.2 Solutions to be Prepared; 4.3 First-day Experiments; 4.3.1 Absorption |
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