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Record Nr. |
UNISA996197616503316 |
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Autore |
Kellner R (Roland) |
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Titolo |
Microcharacterization of proteins [[electronic resource] /] / R. Kellner, F. Lottspeich, H.E. Meyer |
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Pubbl/distr/stampa |
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ISBN |
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1-281-75875-2 |
9786611758752 |
3-527-61571-7 |
3-527-61570-9 |
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Descrizione fisica |
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1 online resource (288 p.) |
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Altri autori (Persone) |
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LottspeichF <1947-> (Friedrich) |
MeyerH. E (Helmut E.) |
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Disciplina |
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Soggetti |
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Proteins |
Proteins - Analysis |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Presented at the first meeting "Mikromethoden in der Proteinchemie" held June 1994 at the Max Planck Institute for Biochemistry. |
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Nota di bibliografia |
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Includes bibliographical references. |
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Nota di contenuto |
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Microcharacterization of Proteins; Contents; Section I: Overview; 1.1 Microcharacterization of Proteins; 1 General Aspects; 2 From a Cell to a Protein Sequence; 3 FutureTrends; Section II: Sample Preparation; II.1 Chemical and Enzymatic Fragmentation of Proteins; 1 Strategy; 2 Denaturation, Reduction and Alkylation; 3 Enzymatic Fragmentation; 3.1 Enzymes; 3.2 Practical Considerations; 4 Chemical Fragmentation; 4.1 Cyanogen Bromide Cleavage; 4.2 Partial Acid Hydrolysis; 4.3 Hydroxylamine Cleavage of Asn-Gly Bonds; 4.4 Cleavage at Tryptophan; 4.5 Cleavage at Cysteine; 5 References |
II.2 Microseparation Techniques I: High Performance Liquid Chromatography1 Introduction; 2 Getting Started; 2.1 Solvents; 2.2 Pump; 2.3 Pre-Column Split; 2.4 Sample Preparation; 2.5 Injector; 2.6 Tubings; 2.7 In-Line Filter, Guard Column; 2.8 Analytical Column; 2.9 Elution; 2.10 Detection; 2.11 Fractionation; 3 Applications; 4 References; II.3 Microseparation Techniques II: Analysis of Peptides and Proteins by Capillary Electrophoresis; 1 Introduction; 2 Theory; 2.1 |
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Capillary Isotachophoresis; 2.2 Capillary Zone Electrophoresis; 2.3 Electroosmotic Flow; 3 Instrumentation; 3.1 Injection |
3.2 Detection4 Applications; 4.1 Peptide Separations; 4.2 Protein Separations; 5 References; II.4 Microseparation Techniques III: Gel Electrophoresis for Sample Preparation in Protein Chemistry; 1 Introduction; 2 Denaturing Techniques; 2.1Commonly Used SDS-Polyacrylamide Gel Electrophoresis Techniques for Protein Separation; 2.2 Blue-SDS-PAGE for Quantitative Protein Recovery from Gels; 2.3 Electroelution of Proteins After Blue-SDS-PAGE; 2.4 Electroblotting of Blue and Colourless SDS Gels; 2.5 Isoelectric Focusing in the Presence of Urea; 3 Native Techniques; 3.1 Colourless-Native-PAGE |
3.2 Blue-Native-PAGE3.3 Native Isoelectric Focusing; 4 References; II.5 Microseparation Techniques IV: Electroblotting; 1 Introduction; 2 Electroblotting; 2.1 Polyacrylamide Gel Electrophoresis; 2.2 Blot Systems; 2.2.1 Tank Blotting; 2.2.2 Semidry Blotting; 2.3 Blotting Parameters; 2.3.1 The Blotting Process; 2.3.2 Transfer Buffers; 2.3.3 Addition of SDS; 2.3.4 Addition of Methanol; 2.3.5 Influence of Protein Concentration; 3 Blotting Membranes; 4 References; Section III: Amino Acid Analysis; III.1 Amino Acid Analysis; 1 Introduction; 2 Sample Preparation; 2.1 Peptides and Proteins |
2.1.1 Enzymatic Hydrolysis2.1.2 Acid Hydrolysis; 2.1.3 Alkaline Hydrolysis; 2.2 Free Amino Acids; 3 Derivatization; 3.1 Post-Column Derivatization; 3.1.1 Ninhydrin; 3.1.2 Orthophthaldialdehyde; 3.1.3 Fluorescamine; 3.2 Pre-Column Derivatization; 3.2.1 Phenylisothiocyanate; 3.2.2 Orthophthaldialdehyde; 3.2.3 Fluorenylmethyl Chloroformate; 3.2.4 Dabsyl Chloride; 3.2.5 Dansyl Chloride; 3.2.6 Chiral Reagents; 4 Data Evaluation; 5 Instrumentation; 6 Discussion; 7 References; Section IV. Protein Sequence Analysis; IV. 1 The Edman Degradation; 1 The Edman Chemistry |
1.1 Coupling, Cleavage and Conversion |
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Sommario/riassunto |
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Both a thorough introduction and laboratory guide!This book is based on the lectures and demonstrations presented at a successful workshop organized by the authors. Expert contributions and the support of more than a dozen companies engaged in bioanalysis and instrumentation enabled the participants to familiarize themselves with the most recent developments e.g. in protein separation and characterization (including laser desorption-ionisation mass spectrometry), fragmentation and micro sequencing.The workshop was held in the Max Planck Institute for Biochemistry at Martinsried |
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2. |
Record Nr. |
UNINA9910830634103321 |
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Titolo |
Novel techniques for imaging the heart : cardiac MR and CT / / edited by Marcelo F. Di Carli, Raymond Y. Kwong |
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Pubbl/distr/stampa |
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Chichester, West Sussex, England ; ; Hoboken, New Jersey : , : Wiley-Blackwell, , 2008 |
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©2008 |
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ISBN |
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1-4443-0059-8 |
1-4443-0324-4 |
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Descrizione fisica |
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1 online resource (384 p.) |
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Collana |
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Disciplina |
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Soggetti |
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Heart - Tomography |
Heart - Magnetic resonance imaging |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Contents; Contributors; Preface; Forewords; PART I General Considerations and Fundamentals of Imaging; 1 Principles of CT and MRI; 2 Clinical Considerations on the Use of Contrast Agents for CT and MRI; 3 Radiation Dose Considerations in Cardiac CT; 4 Safety Considerations of Current and Evolving CMR Techniques and Hardware; PART II Clinical Applications of CT and CMR Imaging; 5 Evaluating the Symptomatic Patient with Suspected CAD; 6 Evaluation of Atherosclerotic Plaques with CT and MRI; 7 Evaluating Chest Pain in Patients with Known CAD |
8 Evaluating the Patient with LV Dysfunction for Potential Revascularization9 Role of Hybrid Imaging: PET/CT and SPECT/CT; 10 Critical Review of Imaging Approaches for the Diagnosis and Prognosis of CAD; 11 Evaluating the Patient with New Onset Heart Failure; 12 Evaluating the Patient before Noncardiac Surgery; 13 Evaluating the Patient before Interventional Electrophysiology; 14 Assessment of Blood Flow and Heart Valve Disease by CMR; 15 Relative Merits of CTA and MRA for Coronary Artery Imaging; 16 Can Atherosclerosis Imaging Improve Patient Management? |
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PART III Advanced Applications of CT and CMR Imaging17 Atherosclerosis Imaging: A Biological and Clinical Perspective; 18 Stem Cell Imaging; 19 Myocardial Imaging in CAD: Beyond Ischemia and Viability; 20 Technical Advances and the Future Prospects of High Field Strength MRI; 21 Technical Advances in MDCT for Imaging Coronary Artery Stenoses and Physiology; 22 Imaging of Myocardial Mechanics; 23 Cardiovascular Interventional MRI; Index; Color Plate |
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Sommario/riassunto |
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This book brings the recent dramatic changes in the field of cardiovascular imaging into the clinical setting to enable the clinician to best use the technology at hand. Novel Techniques for Imaging the Heart opens with three chapters reviewing the general considerations and fundamentals of imaging, followed by a series of chapters that address clinical applications of CT and CMR, includingcritical review of imaging approaches for diagnosis and prognosis of CADevaluating the patient with new onset heart failureevaluating the patient before non-cardiac surgeryevalua |
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