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1. |
Record Nr. |
UNINA9911019893403321 |
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Titolo |
In situ hybridization / / Melody Clark |
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Pubbl/distr/stampa |
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[Saddle River, NJ?, : Wiley-VCH, 2002?] |
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ISBN |
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9786611758547 |
9781281758545 |
128175854X |
9783527615070 |
3527615075 |
9783527615063 |
3527615067 |
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Descrizione fisica |
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1 online resource (182 p.) |
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Collana |
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Altri autori (Persone) |
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Disciplina |
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Soggetti |
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In situ hybridization |
Genetics |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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In Situ Hybridization; Contents; CHAPTER 1. Genomic In Situ Hybridization for Whole Chromosome and Genome Analysis; 1.1 Introduction; 1.2 Uses of Genomic In Situ Hybridization; 1.2.1 Genome Organization of Different Sequence Classes; 1.2.2 Species Identity, Differentiation and Relatedness; 1.2.3 Genomic Stability; 1.2.4 Spatial Organization of Chromosomes; 1.2.5 Chromosome lntrogression and Rearrangements; 1.2.6 Chromosome Behaviour and Meiosis; 1.3 The Principle of Genomic In Situ Hybridization; 1.3.1 Genomic Probing; 1.3.2 Blocking and Competitive Hybridization; 1.4 Materials and Chemicals |
1.4.1 Buffers and Reagents1.4.2 Chemicals; 1.4.3 Equipment; 1.5 Genomic In Situ Hybridization: Protocol; 1.5.1 Chromosome Preparations; 1.5.2 Probe DNA; 1.5.3 Blocking DNA; 1.5.4 In Situ Hybridization Procedure; 1.6 Troubleshooting; CHAPTER 2. Fluorescence In Situ Hybridization: Applications in Gene Mapping and Clinical Diagnostics; 2.1 Introduction; 2.2 Materials and Chemicals; |
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2.2.1 Buffers and Reagents; 2.2.2 Chemicals; 2.2.3 Equipment; 2.3 FISH Using Single Copy Cosmid Probes; 2.3.1 Preparation of Metaphase Chromosomes; 2.3.2 Preparation of Metaphase Chromosome Slides for Hybridization |
2.3.3 Preparation and Labelling of Probes2.3.4 Hybridization of Cosmid Probes; 2.4 Multicolor FISH Using Cosmid Probes; 2.4.1 Labelling of Probes and Hybridization Reactions; 2.5 lnterphase FISH Analysis; 2.5.1 Preparation of lnterphase Nuclear Slides from Short Term Cultures; 2.5.2 Direct Preparation of lnterphase Spreads; 2.5.3 FISH; 2.6 FISH Analysis Using YAC Clones; 2.6.1 Preparation of Metaphase Chromosomes; 2.6.2 Isolation of YAC DNA; 2.6.3 Biotin Labelling of YACs; 2.6.4 FISH; 2.6.5 Microscopy; 2.7 Direct Visual In Situ Hybridization (DIRVISH); 2.7.1 Preparation of Stretched DNA |
2.7.2 FISH Hybridization2.7.3 Detection and Analysis; 2.7.4 Microscopy; 2.8 Troubleshooting; CHAPTER 3. Detection of Nucleic Acids (DNA and RNA) In Situ by Single and Cyclic Primed In Situ labelling (PRINS): Two Alternatives to Traditional In Situ Hybridization Methods; 3.1 Introduction; 3.2 Materials and Chemicals; 3.2.1 Buffers and Reagents; 3.2.2 Chemicals; 3.2.3 Equipment; 3.3 DNA-PRINS with Oligonucleotide Probes; 3.4 PRINS with Ddel Digested Cloned Probes; 3.4.1 Generation of Primers from Cloned Alpha Satellite DNA; 3.4.2 PRlNS Reaction; 3.5 Multicolour-PRINS; 3.6 PRINS-painting |
3.7 PRINS-PCR and Repeated-PRINS of DNA3.7.1 Chromosome Spreads; 3.7.2 Pretreatment of Chromosome Spreads; 3.7.3 Labelling Reaction; 3.8 PRINS-PCR of mRNA; 3.9 Visualization of Hapten-Labelled Nucleotides; 3.9.1 Standard Slides; 3.9.1.1 Biotin; 3.9.1.2 Digoxigenin; 3.9.2 Micro-slides; 3.9.2.1 Detection of Labelled DNA; 3.9.2.2 Detection of Labelled RNA; 3.9.2.3 Alkaline Phosphatase Visualization; 3.9.2.4 Fluorescent Visualization; 3.10 Troubleshooting |
CHAPTER 4. Fluorescence lmmunophenotyping and lnterphase Cytogenetics as a Tool for Investigation of Neoplasms (FICTION): Combined In Situ Hybridization and Fluorescence lmmunophenotyping |
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Sommario/riassunto |
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In situ hybridization is a proven, powerful technique with applications in chromosome and genome analysis, as well as gene expression. Covering a carefully selected range of techniques with immediate and general applications in research and clinical diagnosis, the book starts with genome and DNA mapping, continues through gene expression localization in wholemount and tissue sections, and on to ultrastructural levels. The step-by-step protocols used reflect research in these areas and are all reproducible. |
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2. |
Record Nr. |
UNINA9910968066403321 |
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Titolo |
Race, voting, redistricting, and the constitution : sources and explorations on the Fifteenth Amendment / / edited with introductions by Marsha J. Tyson Darling |
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Pubbl/distr/stampa |
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New York : , : Routledge, , 2001 |
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ISBN |
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1-135-73045-8 |
1-315-05495-7 |
1-135-73038-5 |
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Edizione |
[1st ed.] |
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Descrizione fisica |
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1 online resource (515 p.) |
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Collana |
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Controversies in constitutional law |
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Altri autori (Persone) |
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DarlingMarsha J. Tyson (Marsha Jean Tyson) |
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Disciplina |
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Soggetti |
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Election districts - United States |
Apportionment (Election law) - United States |
Gerrymandering - United States |
Voting - United States |
Race discrimination - Law and legislation - United States |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references. |
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Nota di contenuto |
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Cover; Title Page; Copyright Page; Acknowledgments; Tableof Contents; Series Introduction; Volume Introduction; Enforcement of the Voting Rights Act; Hearings Before the Civil Rights Oversight Subcommittee on the Enforcement of the Voting Rights Act.; Testimony of Aaron Henry et al., NAACP; Hearings Before the Civil Rights Oversight Subcommittee on the Enforcement of the Voting Rights Act.; Testimony of John Lewis, Director, Voter EducationProject; Hearings Before the Civil Rights Oversight Subcommittee on the Enforcement of the Voting Rights Act. |
Testimony of Armand Derfner et al., Lawyer'sCommittee for Civil Rights Under LawTestimony of, and Brief Submitted by, the Mississippi Freedom Democratic Party.; Joseph L. Rauh etal., Leadership Conference on Civil Rights; Correspondence:Frank R. Parker, Lawyer's Committee for Civil Rights Under Law, to Hon. Don Edwards, May 19, 1971; Correspondence: Hon. Don Edwards, Chairman, Civil Rights Oversight Subcommittee, to David L. Norman, Acting Assistant Attorney |
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General, Civil Rights Division, June 1, 1971 |
Correspondence: David L. Norman, Acting Assistant Attorney General, Civil Rights Division,reply to Hon. Don Edwards, Chairman, Civil Rights Oversight Subcommittee and ""Current Registration in Mississippi Counties""Amendments to the Voting Rights Act; Hearings Before the Subcommittee on Constitutional Rights of the Committee on the Judiciary on Amendments to the Voting Rights Act,1970; Statement of Hon. Barry Goldwater; Voting Rights Act Extension, House of Representatives, 94th Congress, 1st Session, Report No. 94-196, May 8, 1975 |
Alternative Voting Systems as Remedies for Unlawful At-Large SystemThe 1982 Amendments to the Voting Rights Act: A Legislative History; The Constitutional Imperative of Proportional Representation; Geometry and Geography: Racial Gerrymandering and the Voting Rights Act; The Disenfranchisement of Ex-Felons: Citizenship, Criminality, and the ""Purity of the Ballot Box""; The Myth of Colorblindness; Book Review: Without Fear and Without Research: Abigail Thernstrom on the Voting Rights Act; Keeping the Courts Honest: The Role of Historians as Expert Witnesses in Southern Voting Rights Cases |
Copyright Acknowledgments |
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Sommario/riassunto |
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First Published in 2002. Routledge is an imprint of Taylor & Francis, an informa company. |
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