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1. |
Record Nr. |
UNINA9911019346903321 |
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Titolo |
Bacterial and eukaryotic porins : structure, function, mechanism / / edited by Roland Benz |
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Pubbl/distr/stampa |
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Weinheim [Germany], : Wiley-VCH, c2004 |
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ISBN |
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9786610519620 |
9781280519628 |
1280519622 |
9783527603879 |
3527603875 |
9783527604227 |
3527604227 |
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Descrizione fisica |
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1 online resource (388 p.) |
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Classificazione |
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Altri autori (Persone) |
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Disciplina |
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Soggetti |
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Molecules |
Proteins - Structure |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Bacterial and Eukaryotic Porins; Contents; Preface; References; List of Contributors; 1 Regulation of Porin Gene Expression by the Two-component Regulatory System EnvZ/OmpR; 1.1 Introduction; 1.2 The Structure of EnvZ; 1.3 Biochemical Activities of EnvZ underlie Signaling; 1.4 What is the EnvZ Activity Regulated by the Stimulus?; 1.5 How is the Signal Propagated?; 1.6 Is there a Role for Acetyl-phosphate in OmpR-P Production?; 1.7 The OmpR Subfamily; 1.8 OmpR Binding Sites; 1.9 Recruitment of RNA Polymerase to OmpR-dependent Promoters; 1.10 OmpR-RNAP Interaction Surface |
1.11 Affinity Model of Porin Gene Regulation1.12 A Test of the Affinity Model; 1.13 Conformational Changes in OmpR Contribute to Differential Regulation of the Porin Genes; 1.14 Other Factors that Regulate ompF and ompC; 1.15 OmpR is a Global Regulator; 1.15.1 Flagellar Biosynthesis; 1.15.2 Curli Fimbriae Production; 1.15.3 Virulence; Acknowledgments; References; 2 The Structures of General Porins; 2.1 Bacterial Outer Membrane Proteins; 2.2 Construction of |
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General Porins; 2.3 Trimer Association and Folding; 2.4 Pore Geometry; 2.5 Permeation; 2.6 Conclusion; Acknowledgments; References |
3 Role of Bacterial Porins in Antibiotic Susceptibility of Gram-negative Bacteria3.1 Introduction; 3.2 Role of Porins in Antibiotic Resistance; 3.2.1 Evolution of Clinical Isolates; 3.2.2 Expression of a Modified Porin; 3.3 In Vitro Mutagenesis Analyses of Porins and Modeling; 3.3.1 Mutations in the Loop 3 Domain; 3.3.2 Mutations in the Anti-loop 3 Domain; 3.3.3 Modeling of β-Lactam in the OmpF Eyelet; 3.4 Conclusion; Acknowledgments; References; 4 Porins of the Outer Membrane of Pseudomonas aeruginosa; 4.1 Introduction; 4.2 The Outer Membrane Permeability Defect in P. aeruginosa |
4.3 Porins Identified in the Genome Sequence4.4 The General Porins; 4.4.1 OprF; 4.4.2 Other General Porins; 4.5 Efflux; 4.5.1 OprM; 4.5.2 OprM Homologs; 4.6 Specific Porins; 4.6.1 OprB; 4.6.2 OprP and OprO; 4.6.3 OprD; 4.6.4 OprD Homologs; 4.7 TonB-dependent Receptors; 4.7.1 FpvA; 4.7.2 FptA; 4.7.3 PfeA and PirA; 4.7.4 HasR, PhuR and OptI; 4.7.5 Other TonB-dependent Receptors; 4.8 Conclusions; Acknowledgments; References; 5 Regulation of Bacterial Porin Function; 5.1 Introduction; 5.2 Voltage Dependence; 5.2.1 L3 and the Constriction Zone; 5.2.2 Extracellular Loops |
5.2.3 Modulation of Voltage Gating5.3 Effect of pH; 5.4 Polyamine Modulation; 5.5 Others; 5.6 Concluding Remarks; Acknowledgements; References; 6 Reconstitution of General Diffusion Pores from Bacterial Outer Membranes; 6.1 Introduction; 6.2 Planar Lipid Bilayer Technique; 6.3 Intrinsic Properties of General Diffusion Channels; 6.3.1 Single-channel Analysis of OmpF Gating; 6.3.2 Molecular Origin of Voltage Gating; 6.3.3 Effect of Membrane Composition and OmpF-LPS Interactions; 6.3.4 Open-channel Conductance; 6.3.5 Voltage Effect and Channel Orientation; 6.3.6 Ion Selectivity |
6.3.7 The Permeating Cations Interact with Specific Elements along the Ionic Pathway |
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Sommario/riassunto |
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This first book dedicated to the topic relates the known physiological functions of porins to their molecular structure and mechanism, as documented by various in vitro and in vivo methods, including the generation of null mutants in mice.For the first time, it brings together biophysical evidence with studies performed in a cellular context, presenting a unified picture of the fundamental importance of porins for cellular function.With 16 contributions by an interdisciplinary team of leading porin researchers, this reference is essential reading for every molecular or structural biolo |
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2. |
Record Nr. |
UNINA9910502987503321 |
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Titolo |
Advances in Application of Stem Cells: From Bench to Clinics / / edited by Firdos Alam Khan |
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Pubbl/distr/stampa |
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Cham : , : Springer International Publishing : , : Imprint : Humana, , 2021 |
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ISBN |
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Edizione |
[1st ed. 2021.] |
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Descrizione fisica |
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1 online resource (320 pages) |
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Collana |
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Stem Cell Biology and Regenerative Medicine, , 2196-8993 ; ; 69 |
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Disciplina |
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Soggetti |
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Stem cells |
Medicine - Research |
Biology - Research |
Regenerative medicine |
Biomedical engineering |
Stem Cell Biology |
Translational Research |
Regenerative Medicine and Tissue Engineering |
Biomedical Engineering and Bioengineering |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Nota di contenuto |
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Basics of stem cell biology -- Types and classifications stem cells -- Method of isolation and characterization of stem cells -- Differentiation of stem cells into neuronal lineage: In vitro cell culture, In vivo transplantation in animal models -- Differentiation of stem cells into cardiomyocyte lineage: In vitro cell culture, In vivo transplantation in animal models -- Differentiation of stem cells into hepatocyte lineage: In vitro cell culture, In vivo transplantation in animal models -- Differentiation of stem cells into pancreatic lineage: In vitro cell culture, In vivo transplantation in animal models -- Application of stem cells in treatment of bone diseases: pre-clinical and clinical perspectives -- Stem cells in regenerative medicine: clinical trials -- Stem cell production: Scale up, GMP production, Bioreactor -- Stem cell based products in the market -- Commercialization, IPR and market of stem |
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Sommario/riassunto |
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The field of stem cell biology is expanding with a continued surge of new information related to its applications. Over past few years, stem cells have been extensively used in cell therapy, tissue engineering, in vitro drug testing among others. At the moment there is no single book available which comprehensively describes the significance of various application of stem cells derived from embryonic and adult sources from lab to clinics. In this edited volume, we discuss basics and advanced topics of stem cells to help researchers, students and professional find the most important information in a single source of updated information about stem cells and relevant applications. This book is divided in 12 chapters and covers topics such as in vitro cell culture, 3D cell culture, cell therapy, tissue engineering, cell factory, cell functionality, in vitro drug testing, organ development, autologous transplantation, allogeneic transplantation, adult stem cells, multipotent stem cells, induced pluripotent stem cells, a pluripotent and embryonic stem cells. |
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