Animal biotechnology : models in discovery and translation / / edited by Prof. Ashish S. Verma, PH.D. (Zoology), Jadavpier University, Kolkata, 700032, W.B., India, Dr. Anchal Singh, PH.D. (Biochemistry), Department of Biochemistry, Institute of Science, Banaras Hindu University, Varanasi, 221005, U.P. India

• Record Nr.: UNINA9910984661503321
• Pubbl/distr/stampa: London, United Kingdom : , : Academic Press, an imprint of Elsevier, , [2020]; ©2020
• ISBN: 9780128117101; 0128117109
• Edizione: [Second edition.]
• Descrizione fisica: 1 online resource (799 pages) : illustrations
• Disciplina: 660.6
• Soggetti: Animal biotechnology; Biomedical engineering; Genetic engineering; Human-animal relationships
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Front Cover -- Animal Biotechnology -- Copyright Page -- Dedication -- Contents -- List of Contributors -- Preface -- Acknowledgments -- I. Human diseases: in vivo and in vitro models -- 1 Drosophila: a model for biotechnologist -- Summary -- What you can expect to know -- Introduction -- Classical aspects of Drosophila melanogaster -- Physical appearance -- Life cycle -- Drosophila development -- Embryogenesis in Drosophila -- Pattern formation in Drosophila -- Homeotic genes in Drosophila -- Drosophila genome -- History -- Historical perspective of Drosophila contributions to biotechnology -- Principle -- Methodology -- Culturing of Drosophila -- Preparation of Drosophila food medium -- Materials required -- Handling of flies -- Fly disposal -- Egg collection -- Dechorination of eggs -- Preparation of DNA for injection -- Protocols -- Protocol for germ-line transformation in Drosophila -- Materials required -- Procedure -- ; Ethical issues -- Translational significance -- Clinical significance -- Drosophila-based models for understanding human neurodegenerative diseases -- Drosophila as a model for understanding human metabolic disorders -- Drosophila as a model for understanding nephrolithiasis (kidney stones) -- Drosophila-based model for understanding the human immunodeficiency virus pathology -- Drosophila-based therapeutic peptide production -- Turning point -- World Wide Web resources -- Acknowledgments -- References -- Further reading -- Glossary -- Abbreviations -- Long answer questions -- Short answer questions -- Answers to short answer questions -- Yes/no type questions -- Answers to yes/no type questions -- 2 Animal models of tuberculosis -- Summary -- Introduction -- Comparative pathology of tuberculosis in humans and animals -- Characteristics of a model for tuberculosis with respect to infection and pathogenesis.; Pathogen diversity: crossing species barriers -- Host diversity: fundamental processes and fine-tuning -- Animal models of tuberculosis: limits and lessons -- Animal models: contributions in tuberculosis vaccine testing -- Various animal models -- Mouse model -- Guinea pig model -- Rabbit model -- Nonhuman primate model -- Cattle model -- Protocols -- Preparing M. tuberculosis inoculum for aerosol exposure -- Aerosol infection of mice using the middlebrook apparatus -- Aerosol infection of guinea pigs using a madison chamber -- Bacteria loading -- Intravenous infection of mice with M. tuberculosis -- Isolation of samples for determining M. tuberculosis load by real-time-PCR -- Determination of bacterial loads in target organs -- Preparation of lungs or other tissues for histology -- Preparation of lung cell suspension -- Ethical issues -- Translational significance -- World wide web resources -- Safety considerations -- References -- Further reading -- Glossary -- Abbreviations -- Long-answer questions -- Short-answer questions -- Answers to short-answer questions -- Yes/no-type questions -- Answers for yes/no type questions -- 3 Animal models for neurodegenerative disorders -- Summary -- What you can expect to know -- History and methods -- Introduction -- Neurodegenerative diseases -- Amyotrophic lateral sclerosis -- Spinal muscular atrophy -- Spinal and bulbar muscular atrophy -- Principles -- Genetics of amyotrophic lateral sclerosis -- Superoxide dismutase 1-amyotrophic lateral sclerosis -- Amyotrophic lateral sclerosis: genes implicated in RNA metabolism -- TAR-DNA-binding protein 43 -- Fused-in sarcoma/translocated in liposarcoma protein -- C9orf72 -- Genetics of spinal muscular atrophy -- Genetics of spinal and bulbar muscular atrophy -- Methodology -- Generation of transgenic mice.; Preparation and purification of transgenic construct (step 1) -- Harvesting donor eggs (step 2) -- Microinjection of transgene to fertilized egg (step 3) -- Implantation of microinjected egg to pseudopregnant female mice (step 4) -- Screening of founder mice for expression of transgene (step 5) -- Establishing stable transgenic line (step 6) -- Cre-loxP technology -- Amyotrophic lateral sclerosis models -- SOD1G37R transgenic mice -- SOD1G93A transgenic mice -- SOD1WT transgenic mice -- Spinal muscular atrophy models -- Severe spinal muscular atrophy mice (mSMN−/− -- SMN2+/+) -- Spinal muscular atrophy type II mice (mSMN−/− -- SMN2+/+ -- SMNΔ7+/+) -- Spinal and bulbar muscular atrophy models -- AR-97Q and AR-24Q transgenic mice -- Examples and their applications -- Superoxide dismutase 1-linked amyotrophic lateral sclerosis -- Gain of toxicity from mutant superoxide dismutase 1 established as pathomechanisms through engineering mutant superoxide di... -- Evaluating phenotype and clinical course of mutant superoxide ; dismutase 1 transgenic mice -- Toxicity from misfolded mutant superoxide dismutase 1 protein -- Non-cell autonomous neurodegeneration demonstrated by superoxide dismutase 1 mouse models -- Stem cell-derived motor neurons established from mutant superoxide dismutase 1 mice -- Other amyotrophic lateral sclerosis -- Spinal muscular atrophy -- Human SMN2 transgenic mice -- Neuron-specific deletion of survival of motor neuron in mice using Cre-loxP systems -- Spinal and bulbar muscular atrophy -- AR-97Q mice as spinal and bulbar muscular atrophy Model -- Androgen hormone and mutant androgen receptor central to spinal and bulbar muscular atrophy pathogenesis -- Clinical correlations -- Protocols -- Ethical issues -- Translational significance -- World Wide Web resources -- Acknowledgment -- References -- Further reading -- Glossary.; Abbreviations -- Long answer questions -- Short answer questions -- Answers to short answer questions -- Yes/no type questions -- Answers for yes/no type questions -- 4 Epigenetics and animal models: applications in cancer control and treatment -- Summary -- What you expect to know -- Introduction -- History -- Principle -- Use of mouse models in the epigenetics of cancer -- Examples with applications -- Brain cancer -- Breast cancer -- Colorectal cancer -- Esophageal cancer -- Gastric cancer -- Head and neck cancer -- Lung cancer -- Lymphoma and leukemia -- Prostate cancer -- Liver cancer -- Other approaches -- Methodology -- Methylation profiling -- Histone profiling -- Nucleosome mapping -- Protocols -- Ethical issues -- Translation significance -- Clinical significance -- Web resources -- Turning point -- Flow chart -- World Wide Web resources -- Something interesting about this chapter -- References -- Glossary (terms used in text with examples) -- Abbreviations -- Long answer questions -- Answers to long answer questions -- Short answer questions -- Answers to short answer questions -- Yes/no type questions -- Answers to yes/no type questions -- 5 Development of mouse models for cancer research -- Summary -- What you can expect to know -- Introduction -- History -- Principle -- Institutional Animal Care and Use Committee approval -- Institutional Animal Care and Use Committee guidelines -- Methodology -- Inbred mice -- Examples with applications -- Immunocompetent mice -- Spontaneous tumor models -- The genetically engineered mouse models -- The Cre/Lox system: a superior genetically engineered mouse model -- Immunodeficient mice -- Allograft transplants -- Xenograft transplants -- Humanized mice -- Checklist for a successful in vivo experiment -- Protocols -- An orthotopic mouse model of colorectal cancer -- Design and execution.; Interpretation of results -- A xenograft model of prostate cancer metastasis -- Design and execution -- Interpretation of results -- Humanized mouse models for tumor xenografts -- Design and execution -- Ethical issues -- Turning point -- Translational significance -- World Wide Web resources -- References -- Glossary -- Abbreviations -- Long answer questions -- Short answer questions -- Answers to short answer questions -- Yes/no type questions -- Answers to yes/no type questions -- 6 The clinico-molecular approaches for detection of human papillomavirus -- Summary -- What you can expect to know -- Introduction -- Cancer -- Cervical cancer -- Historical overview -- Mistaken theories of cervical cancer causation -- The first breakthrough -- zur Hausen -- Prevalence and epidemiology of cervical cancer -- Global scenario -- Symptoms of cervical cancer -- Anatomy of female pelvis -- Types of cervical cancer -- Risk factors for cervical cancer -- Human papillomaviruses -- Genomic organization of human papillomavirus -- Transcriptional ; regulation of human papillomavirus -- Life cycle of human papillomavirus -- Functions of human papillomavirus oncoproteins E6 and E7 -- Inactivation and degradation of p53 through the E6/E6AP complex -- Screening and diagnostic methodologies of cervical cancer -- Screening -- Methods used for screening/diagnosis of cervical cancer -- Visual methods -- Indications -- Other screening tests -- Reporting systems terminology -- Precancer classification -- WHO classification -- CIN classification (Bhambhani., 2007) -- Bethesda classification -- Cancer classification -- Colposcopy and biopsy -- New technologies -- DNA cytometry -- Human papillomavirus DNA-based screening methods (protocol) -- Urine-based noninvasive human papillomavirus DNA detection method.; Simple "paper smear" method for rapid detection of human papillomavirus infection.
• Sommario/riassunto: Animal Biotechnology: Models in Discovery and Translation, Second Edition, provides a helpful guide to anyone seeking a thorough review of animal biotechnology and its application to human disease and welfare.