Methods in bioengineering : biomicrofabrication and biomicrofluidics / / Jeffrey D. Zahn, editor

• Record Nr.: UNINA9910780919503321
• Pubbl/distr/stampa: Boston : , : Artech House, , ©2010; [Piscataqay, New Jersey] : , : IEEE Xplore, , [2009]
• ISBN: 1-5231-4631-1; 1-59693-401-8
• Descrizione fisica: 1 online resource (366 p.)
• Collana: Artech House methods in bioengineering series
• Altri autori (Persone): ZahnJeffrey D
• Disciplina: 610.284
• Soggetti: Bioengineering - Methodology; Microfabrication; Microfluidics
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Methods in Bioengineering: Biomicrofabrication and Biomicrofluidics; Contents; Preface; Chapter 1 Microfabrication Techniques for Microfluidic Devices; 1.1 Introduction to microsystems and microfluidic devices; 1.2 Microfluidic systems: fabrication techniques; 1.3 Transfer processes; 1.3.1 Photolithography; 1.3.2 Molding; 1.4 Additive processes; 1.4.1 Growth of SiO2; 1.4.2 Deposition techniques; 1.5 Subtractive techniques; 1.5.1 Etching; 1.5.2 Chemical-mechanical polishing and planarization; 1.6 Bonding processes; 1.6.1 Lamination; 1.6.2 Wafer bonding methods; 1.7 Sacrificial layer techniques; 1.8 Packaging processes1.8.1 Dicing; 1.8.2 Electrical interconnection and wire bonding; 1.8.3 Fluidic interconnection in microfluidic systems; 1.9 Materials for microfluidic and bio-MEMS applications; 1.9.1 Glass, pyrex, and quartz; 1.9.2 Silicon; 1.9.3 Elastomers; 1.9.4 Polydimethylsiloxane; 1.9.5 Epoxy; 1.9.6 SU-8 thick resists; 1.9.7 Thick positive resists; 1.9.8 Benzocyclobutene; 1.9.9 Polyimides; 1.9.10 Polycarbonate; 1.9.11 Polytetrafluoroethylene; 1.10 Troubleshooting table; 1.11 Summary; References; Chapter 2 Micropumping and Microvalving; 2.1 Introduction; 2.2 Actuators for micropumps and microvalves2.2.1 Pneumatic ; actuators; 2.2.2 Thermopneumatic actuators; 2.2.3 Solid-expansion actuators; 2.2.4 Bimetallic actuators; 2.2.5 Shape-memory alloy actuators; 2.2.6 Piezoelectric actuators; 2.2.7 Electrostatic actuators; 2.2.8 Electromagnetic actuators; 2.2.9 Electrochemical actuators; 2.2.10 Chemical actuators; 2.2.11 Capillary-force actuators; 2.3 Micropumps; 2.3.1 Mechanical pump; 2.3.2 Nonmechanical pump; 2.4 Microvalves; 2.4.1 Mechanical valve; 2.4.2 Nonmechanical valve; 2.5 Outlook; 2.6 Troubleshooting; 2.7 Summary points; References; Chapter 3 Micromixing Within Microfluidic Devices3.1 Introduction; 3.2 Materials; 3.2.1 Microfluidic mixing devices; 3.2.2 Microfluidic interconnects; 3.2.3 Optical assembly; 3.2.4 Required reagents; 3.3 Experimental design and methods; 3.3.1 Passive micromixers; 3.3.2 Active micromixers; 3.3.3 Multiphase mixers; 3.4 Data acquisition, anticipated results, and interpretation; 3.4.1 Computer acquisition; 3.4.2 Performance metrics, extent of mixing, reaction monitoring; 3.5 Discussion and commentary; 3.6 Troubleshooting; 3.7 Application notes; 3.8 Summary points; References; Chapter 4 On-Chip Electrophoresis and Isoelectric Focusing Methods for Quantitative Biology4.1 Introduction; 4.1.1 Microfluidic electrophoresis supports quantitative biology and medicine; 4.1.2 Biomedical applications of on-chip electrophoresis; 4.2 Materials; 4.2.1 Reagents; 4.2.2 Facilities/equipment; 4.3 Methods; 4.3.1 On chip polyacrylamide gel electrophoresis (PAGE); 4.3.2 Polyacrylamide gel electrophoresis based isoelectric focusing; 4.3.3 Data acquisition, anticipated results, and interpretation; 4.3.4 Results and discussion; 4.4 Discussion of pitfalls; 4.5 Summary notes
• Sommario/riassunto: Written and edited by recognized experts in the field, the new Artech House Methods in Bioengineering series offers detailed guidance on authoritative methods for addressing specific bioengineering challenges. Offering a highly practical presentation of each topic, each book provides research engineers, scientists, and students with step-by-step procedures, clear examples, and effective ways to overcome problems that may be encountered. This unique volume presents leading-edge microfluidics methods used to handle, manipulate, and analyze cells, particles biological components (e.g., proteins and DNA) for microdiagnostics.

Suspicion / / Laura Grimaldi ; translated by Robin Pickering-Iazzi

• Record Nr.: UNINA9910969930603321
• Autore: Grimaldi Laura
• Pubbl/distr/stampa: Madison, Wis., : University of Wisconsin Press, Terrace Books, c2003
• ISBN: 9786612424250; 9781282424258; 1282424254; 9780299188634; 0299188639
• Edizione: [1st ed.]
• Descrizione fisica: 1 online resource (258 p.)
• Altri autori (Persone): Pickering-IazziRobin
• Disciplina: 853/.914
• Soggetti: Murder; Fiction
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Bibliographic Level Mode of Issuance: Monograph
• Nota di contenuto: Intro -- Chapter 1 -- Chapter 2 -- Chapter 3 -- Chapter 4 -- Chapter 5 -- Chapter 6 -- Chapter 7 -- Chapter 8 -- Chapter 9 -- Chapter 10 -- Chapter 11 -- Chapter 12 -- Chapter 13 -- Chapter 14 -- Chapter 15 -- Chapter 16 -- Chapter 17 -- Chapter 18 -- Chapter 19 -- Chapter 20 -- Chapter 21 -- Chapter 22 -- Chapter 23 -- Chapter 24 -- Chapter 25 -- Chapter 26 -- Chapter 27.
• Sommario/riassunto: In the shadow of the "Monster of Florence, " a serial murderer who has terrorized Italy for seventeen years, Laura Grimaldi sets her tense psychological thriller Suspicion -a noir mystery of a city transformed by fear, and of friendships and family ties twisted by uncertainty and dark speculation. Grimaldi, whose hardboiled mysteries of the 1950s earned her the title "Italy's queen of crime, " turns here to the deeper, more elusive and disturbing questions that haunt human affairs. For years Matilde, the widow of a prominent Florentine doctor, has lived alone with her eccentric middle-aged son, Enea. When the police pay a call, the balance between mother and son is shifted just subtly enough to make Matilde prey to suspicions and doubts that grow ever more corrosive, ever harder to conceal and more dangerous to reveal. In the literary tradition of such mystery writers as Patricia Highsmith and Ruth ; Rendell, Grimaldi creates an atmosphere charged with suspense as the daily lives and routines of her characters, infected with suspicion, begin to rearrange themselves around a few frightening facts and infinite monstrous possibilities. Matilde's efforts to decipher Enea's secretive movements and occupations appear perfectly sensible and defensible through Grimaldi's deft shifts between mother and son-and another, chillingly detached perspective on the gruesome murders. Grimaldi's readers will find themselves as subject to misinterpretation and doubt, to sympathies and suspicions as her Florentine characters, and spellbound until the book's final page.