Insect cell cultures : fundamental and applied aspects / / edited by J.M. Vlak ... [et al.]

• Record Nr.: UNINA9910966640403321
• Pubbl/distr/stampa: Dordrecht [Netherlands] ; ; Boston, : Kluwer, c1996
• ISBN: 1-280-04313-X; 9786610043132; 0-306-46850-6
• Edizione: [1st ed. 2002.]
• Descrizione fisica: 1 online resource (325 p.)
• Collana: Current applications in cell culture engineering ; ; vol. 2
• Altri autori (Persone): VlakJ. M
• Disciplina: 660/.6
• Soggetti: Insect cell biotechnology; Insects - Viruses; Insects - Cytology
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Insect Cell Lines -- Development and characterization of insect cell lines -- New approaches to insect tissue culture -- Transgenic insect cells: mosquito cell mutants and the dihydrofolate reductase gene -- Insect cell physiology -- Insect cell cultivation: growth and kinetics -- Medium design for insect cell culture -- Viruses -- Baculovirus-insect cell interactions -- Replication patterns and cytopathology of cells infected with baculoviruses -- Construction of baculovirus recombinants -- Passage effect of virus infection in insect cells -- Post-translational modifications in insect cells -- Productivity of insect cells for recombinant proteins -- Chaperone and foldase coexpression in the baculovirus-insect cell expression system -- Engineering Aspects -- Shear sensitivity of insect cells -- Insect cell bioreactors -- Perfusion bioreactors for the production of recombinant proteins in insect cells -- Immobilization of insect cells -- Modelling baculovirus infection of insect cells in culture -- Scale up aspects of sparged insect-cell bioreactors -- Oxygen gradients in small and big sparged insect-cell bioreactors -- Downstream processing of insect cell cultures -- Applications -- Parvovirus diagnostics and vaccine production in insect cells -- Classical swine fever virus diagnostics and vaccine ; production in insect cells -- Production of multidomain complement glycoproteins in insect cells -- Economic and Regulatory Aspects -- Economics of baculovirus-insect cell production systems -- Safety aspects of insect cell culture -- Regulatory issues in the use of insect-cell culture.
• Sommario/riassunto: A comprehensive reference work covering the key issues in insect cell cultures, this text includes 30 review papers on such topics as: cell lines (development, characterisation, physiology, cultivation and medium design); viruses (virus-cell interactions, replication, recombinant construction, infection kinetics, post-translational modification and passage effects); engineering (shear, bioreactors including perfusion, immobilisation, scale-up and modelling, downstream processing); applications; and economics and regulatory aspects.; This text should be useful for cell biologists, biochemists, molecular biologists, virologists, immunologists and other basic and applied disciplines related to cell culture engineering, both academic and industrial.