Caco-2 cells and their uses / / Megan A. Schulz, editor

• Record Nr.: UNINA9910966245903321
• Pubbl/distr/stampa: Hauppauge, N.Y., : Nova Science Publishers, Inc., c2011
• ISBN: 1-62081-613-X
• Edizione: [1st ed.]
• Descrizione fisica: 1 online resource (219 p.)
• Collana: Cell biology research progress
• Altri autori (Persone): SchulzMegan A
• Disciplina: 611/.0187
• Soggetti: Epithelial cells; Monomolecular films; Intestinal absorption
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Intro -- CACO-2 CELLS AND THEIR USES -- CACO-2 CELLS AND THEIR USES -- Library of Congress Cataloging-in-Publication Data -- CONTENTS -- PREFACE -- Chapter 1 THE INDUSTRIAL AND ACADEMIC RATIONALE OF CACO-2 CELL MODELS TODAY -- ABSTRACT -- ABBREVIATIONS -- 1. INTRODUCTION -- 2. CACO-2 CELL ADVANTAGES, DRAWBACKS AND TRANSPORTER-ENZYME EXPRESSION PATTERNS -- 2.1. Applicability of CACO-2 Models to Predict the Fraction of Drug Absorbed in Humans -- 2.2. CACO-2 Transporters and Enzyme Expression and the Comparison with the In Vivo Human Expression -- 3. CACO-2 CELLS AS AN IN VITRO PROGNOSTIC TOOL FOR ASSESSING PHARMACOKINETIC DRUG-GARLIC INTERACTIONS -- 4. CACO-2 CELLS FOR THE EVALUATION OF SELF-MICROEMULSIFYING DRUG DELIVERY SYSTEMS (SMEDDS) -- 4.1. Self-Microemulsifying Drug Delivery Systems:The Objectives and Advantages -- 4.2. Studies Performed to Evaluate SMEDDS Impact on Intestinal Absorption of Drugs -- 4.3. The Mechanism Responsible for Increased Absorption of Furosemide Incorporated in SMEDDS -- CONCLUSION -- REFERENCES -- Chapter 2 A CRITICAL PERSPECTIVE ON CELL LINES STUDIES IN NUTRITION: THE CASE OF INTESTINAL ABSORPTION -- ABSTRACT -- INTRODUCTION -- RESEARCH WITH CELL LINES -- CELL LINES IN INTESTINAL ABSORPTION STUDIES -- NON-HUMAN CELL LINES IN INTESTINAL ABSORPTION STUDIES -- IN VITRO CELL CULTURES AS AN ; ALTERNATIVE TO ANIMAL TESTING -- INTESTINAL CELL LINES AS AN IN VITRO MODEL OF CLINICAL VALUE -- CONCLUSION -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 3 CO-CULTURE SYSTEMS WITH CACO-2 CELLS TO EVALUATE THE BIOACTIVITY OF PLANT EXTRACTS -- ABSTRACT -- INTRODUCTION -- CO-CULTURE SYSTEMS USING CACO-2 CELLS: CELL VIABILITY AND FUNCTIONALITY -- WORKING WITH NATURAL EXTRACTS: CACO-2 AS A FILTER TO SIMULATE THE HUMAN INTESTINAL BARRIER -- WORKING WITH NATURAL EXTRACTS: ARE THESE FILTERED EXTRACTS BIOACTIVE? -- CONCLUSION.; ACKNOWLEDGMENTS -- REFERENCES -- Chapter 4 THE USE OF CACO-2 CELLS IN THE STUDY OF INTESTINAL TRANSPORT -- ABSTRACT -- INTRODUCTION -- USES OF CACO-2 CELLS -- Paracellular Pathway -- Transcellular Pathway -- CONCLUSION -- REFERENCES -- Chapter 5 CACO-2 CELLS AS AN EXPERIMENTAL MODEL FOR THE STUDY OF CELL-MATRIX INTERACTIONS AND WOUND HEALING IN INTESTINAL VILLUS CELLS -- ABSTRACT -- INTRODUCTION -- CACO-2 CELLS AND CELL-MATRIX INTERACTIONS -- CACO-2 CELLS AND CELL-MATRIX INTERACTIONS IN CO-CULTURE -- CACO-2 CELLS IN CO-CULTURE: A WOUND HEALING MODEL -- CONCLUSIONS -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 6 EFFECT OF POLYUNSATURATED FATTY ACIDS AND BILE SALTS ON BUTYRATE UPTAKE BY INTESTINAL EPITHELIAL CELLS -- ABSTRACT -- INTRODUCTION -- MATERIALS AND METHODS -- CACO-2 Cell Culture -- IEC-6 Cell Culture -- Determination of 14C-BT uptake by CACO-2 and IEC-6 Cells -- Treatment of the Cells with Compounds -- Determination of Cell Viability -- Protein Determination -- Calculation and Statistics -- Materials -- RESULTS -- Effect of Pufas and Bile Acids on 14C-BT Apical uptake by CACO-2 Cells -- Effect of Pufas and Bile Acids on 14C-BT Apical uptake by IEC-6 Cells -- CONCLUSION -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 7 ANALYSIS OF BOTULINUM TOXIN: INTESTINAL EPITHELIAL BARRIER INTERACTION USING EPITHELIAL MONOLAYER CULTURE SYSTEMS -- ABSTRACT -- INTRODUCTION -- 1. TRANSCYTOSIS OF BONT ACROSS CULTURED INTESTINAL EPITHELIAL MONOLAYERS -- 2. ADHESION OF HA TO INTESTINAL EPITHELIAL CELLS -- 3. DISRUPTION OF THE INTERCELLULAR EPITHELIAL BARRIER BY HA -- 4. POSSIBLE MECHANISMS OF BONT COMPLEX TRANSPORT ACROSS THE INTESTINAL EPITHELIAL MONOLAYER -- CONCLUSION -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 8 CACO-2 CELLS AND GENOTOXICITY STUDIES OF FOOD CONTAMINANTS -- ABSTRACT -- INTRODUCTION ON IN VITRO GENOTOXICITY TESTING.; IN VITRO HUMAN INTESTINAL CELLS: PERTINENCE FOR GENOTOXICITY TESTING OF FOOD CONTAMINANTS -- USE OF CACO-2 CELLS IN GENOTOXICITY TESTS -- Comet Assay -- Others -- CYTOKINESIS-BLOCK MICRONUCLEUS ASSAY -- On Undifferentiated Cells -- On Differentiated Cells: Development and Application -- IMPROVEMENT OF THIS CELL MODEL TO PREDICT TOXICITY AND MECHANISTIC ENDPOINTS -- Co-Cultures CACO-2/TK6 -- Metabolism Regulation -- CONCLUSIONS -- ACKNOWLEDGMENT -- REFERENCES -- Chapter 9 CACO-2 CELLS FOR STUDYING INTESTINAL ABSORPTION MECHANISMS: COMPARISON AND CORRELATIONS WITH RAT MODEL -- ABSTRACT -- INTRODUCTION: IN VITRO MODELS OF INTESTINAL PERMEATION -- ABSORPTION IN THE GASTROINTESTINAL TRACT: ROUTES OF PERMEATION ACROSS INTESTINAL CELLS. -- IN VITRO MODELS OF THE INTESTINAL BARRIER: CELL MODELS -- CACO-2 -- Other Cell Lines -- HT29 Cells -- CACO-2 CELLS FOR PREDICTING ORAL FRACTION ABSORBED: COMPARISON WITH HUMAN, RAT AND PAMPA MODELS -- CACO-2 CELLS FOR THE STUDY OF DRUG ABSORPTION MECHANISMS: ; MODELING APPROACHES FOR PARAMETER ESTIMATION -- Apparent Permeability Estimation (Sink versus Non Sink Conditions) -- Mathematical Modeling of CACO-2 Data for Parameter Estimation -- P versus C -- Amounts versus Time -- CORRELATION BETWEEN CACO-2 CELLS DATA AND IN SITU RAT DATA FOR THE STUDY OF P-GP MEDIATED SECRETION -- CONCLUSIONS -- REFERENCES -- Chapter 10 NANOCARRIER ABSORPTION STUDIES WITH CACO-2 CELLS -- ABSTRACT -- INTRODUCTION -- I. METHOD -- a. Absorption Study -- b. Mechanistic Studies -- II. RESULTS -- a. Absorption Study -- b. Mechanistics Study -- III. DISADVANTAGE/ LIMIT OF CACO-2 TO STUDY DRUG TRANSPORT / OTHER METHOD -- a. Parallel Artificial Membrane Permeation Assay (PAMPA) -- b. Alternative Cell Culture Models -- c. In Vivo or Ex Vivo Studies -- CONCLUSION -- ACKNOWLEDGMENT -- BIBLIOGRAPHY.; Chapter 11 CACO-2 CELLS AS A MODEL TO STUDY THE INTESTINAL EFFECTS OF NON-ABSORBABLE PHYTOCHEMICALS -- ABSTRACT -- ABBREVIATIONS -- 1. INTRODUCTION -- 2. PROCYANIDINS:STRUCTURE AND METABOLISM -- 3. PROCYANIDINS AND THE HEALTH OF THE GASTROINTESTINAL TRACT -- 4. INTERACTIONS OF PROCYANIDINS WITH CACO-2 CELL MEMBRANES -- 5. PROCYANIDINS PROTECTCACO-2 CELLSFROM OXIDATION AND PERMEABILIZATION -- 6. PROCYANIDINS PROTECTCACO-2 CELLS FROM INFLAMMATION -- CONCLUSION -- ACKNOWLEDGMENTS -- REFERENCES -- INDEX.
• Sommario/riassunto: Presents topical research in the study of CACO-2 cells and their uses. This book discusses topics that include the application of CACO-2 cell models as a supportive in vitro tool during drug discovery and formulation; co-culture systems with CACO-2 cells to evaluate the bioactivity of plant extracts; and, more.