3D cell-based biosensors in drug discovery programs : microtissue engineering for high throughput screening / / William S. Kisaalita

• Record Nr.: UNINA9910958307103321
• Autore: Kisaalita William Ssempa <1953->
• Pubbl/distr/stampa: Boca Raton, [FL], : CRC Press, 2010
• ISBN: 1-4398-5905-1; 0-429-14677-9; 1-4200-7350-8
• Edizione: [1st ed.]
• Descrizione fisica: 1 online resource (392 p.)
• Disciplina: 615/.19
• Soggetti: Pharmaceutical biotechnology; Biosensors; High throughput screening (Drug development)
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Front Cover; Dedication; Contents; Preface; Author; Part I. Introduction; Chapter 1. Biosensors and Bioassays; Chapter 2. Target-Driven Drug Discovery; Part II. 3D versus 2D Cultures; Chapter 3. Comparative Transcriptional Profiling and Proteomics; Chapter 4. Comparative Structure and Function; Part III. Emerging Design Principles; Chapter 5. Chemical Microenvironmental Factors; Chapter 6. Spatial and Temporal Microenvironmental Factors; Chapter 7. Material Physical Property and Force Microenvironmental Factors; Chapter 8. Proteomics as a Promising Tool in the Search for 3D Biomarkers; Chapter 9. Readout Present and Near FutureChapter 10. Ready-to-Use Commercial 3D Plates; Part IV. Technology Deployment Challenges and Opportunities; Chapter 11. Challenges to Adopting 3D Cultures in HTS Programs; Chapter 12. Cases for 3D Cultures in Drug Discovery; Chapter 13. Ideal Case Study Design; Appendix A: Patents for 3D Scaffolds; Appendix B: Current Drug Targets; Appendix C: Popular Cell Lines in Drug Discovery; Appendix D: Stem Cells in Drug Discovery; Back Cover
• Sommario/riassunto: This book is based upon cutting-edge research conducted in the authors lab (Cellular Bioengineering), which over the past decade has ; developed a number of sophisticated techniques to facilitate use of 3D cell based assays or biosensors. This book uses data from peer-reviewed publications to conclusively justify use of 3D cell cultures in cell-based biosensors (assays) for (HTS). The majority of assays performed in accelerated drug discovery processes are biochemical in nature, but there is a growing demand for live cell-based assays. Unlike biochemical ones, cellular assays are functional approximations of in vivo biological conditions and can provide more biologically relevant information--Provided by publisher.