Record Nr.

UNINA9910956907403321

Titolo

Upstream industrial biotechnology / / edited by Michael C. Flickinger

Pubbl/distr/stampa


Hoboken, New Jersey, : John Wiley & Sons Inc., c2013

ISBN

9781118619278

1118619277

9781118619230

1118619234

Edizione

[1st ed.]

Descrizione fisica

1 online resource (1838 p.)

Classificazione

SCI010000

Altri autori (Persone)

FlickingerMichael C

Disciplina

660.6

Soggetti

Biotechnology

Biotechnology industries

Lingua di pubblicazione

Inglese

Formato

Materiale a stampa

Livello bibliografico

Monografia

Note generali

Description based upon print version of record.

Nota di bibliografia

Includes bibliographical references and index.

Nota di contenuto

UPSTREAM INDUSTRIAL BIOTECHNOLOGY; CONTENTS; 27 Animal Cell Culture, Effects of Osmolality and Temperature; PREFACE; CONTRIBUTORS; PART I INTRODUCTION; PART II INDUSTRIAL CELL GROWTH AND GENE EXPRESSION SYSTEMS; 1 Animal Cells, Suspension Culture; 1.1 INTRODUCTION; 1.2 TYPES USED FOR LARGE-SCALE PRODUCTION IN SUSPENSION CULTURE; 1.3 SUSPENSION CULTURE REACTORS; 1.4 OPERATING MODES FOR REACTORS; 1.5 PROCESS MONITORING AND CONTROL; 1.6 CULTURE MEDIA FOR SUSPENSION CULTURE; 1.7 CONCLUSIONS; REFERENCES; 2 Baculovirus Expression Systems; 2.1 INTRODUCTION; 2.2 BACULOVIRUS STRUCTURE AND REPLICATION

2.3 PRODUCTION OF RECOMBINANT BACULOVIRUSES2.4 BACULOVIRUS TRANSFER VECTORS; 2.5 MODIFYING THE BACULOVIRUS GENOME TO IMPROVE PROTEIN PRODUCTION; 2.6 INSECT CELL CULTURE; 2.7 BACULOVIRUSES FOR GENE EXPRESSION IN MAMMALIAN CELLS; 2.8 CONCLUSION; REFERENCES; 3 Baculovirus Kinetics, Insect Culture; 3.1 HISTORY AND CHALLENGE; 3.2 BACULOVIRUS; 3.3 CELL YIELD CONCEPT; 3.4 KINETIC MODEL OF VIRAL INFECTION: SYNCHRONOUS INFECTION; 3.5 KINETIC MODEL OF VIRAL INFECTION: ASYNCHRONOUS INFECTION; REFERENCES; 4 Cell Culture, Aseptic Techniques; 4.1

INTRODUCTION; 4.2 ASEPTIC TECHNIQUE: GENERAL CONSIDERATIONS

4.3 ASEPTIC TECHNIQUE: BASIC PROCEDURES4.4 HEPA FILTRATION; 4.5 HOODS AND CABINETS EMPLOYING HEPA FILTRATION; 4.6 WORKING WITHIN UNIDIRECTIONAL AIRFLOW CABINETS AND MICROBIOLOGICAL SAFETY CABINETS; 4.7 TESTING OF CLASS I AND CLASS II MICROBIOLOGICAL SAFETY CABINETS; 4.8 CLEANROOMS FOR CELL CULTURE USE; REFERENCES; 5 Cell Cycle in Bioprocesses; 5.1 INTRODUCTION; 5.2 THE CELL CYCLE; 5.3 METHODS FOR DESCRIBING THE CELLCYCLE; 5.4 IMPORTANCE OF THE CELL CYCLE IN PROCESS BIOTECHNOLOGY; REFERENCES; 6 Cell Growth and Protein Expression Kinetics; 6.1 INTRODUCTION; 6.2 BATCH CULTURE KINETICS

6.3 CONTINUOUS CULTURE KINETICS6.4 FED-BATCH AND PERFUSION CULTURES; 6.5 CONCLUSIONS; NOMENCLATURE; REFERENCES; 7 Cell Viability Measurement; 7.1 INTRODUCTION; 7.2 PERMEABILITY ASSAYS; 7.3 FUNCTIONAL ASSAYS; 7.4 FLOW CYTOMETRY; 7.5 PHYSICAL METHODS; REFERENCES; 8 Contamination Detection in Animal Cell Culture; 8.1 INTRODUCTION; 8.2 HISTORICAL PERSPECTIVES; 8.3 REGULATORY ISSUES; 8.4 MANUFACTURING AND SAFETY TESTING STANDARDS; 8.5 EXAMPLES OF VIRAL CONTAMINANTS; 8.6 DETECTION OF VIRAL CONTAMINANTS IN CELL LINES; 8.7 TESTING RAW MATERIALS; 8.8 DETECTION OF MYCOPLASMAS; 8.9 BACTERIA AND FUNGI

8.10 OXYGEN UPTAKE RATE8.11 ENDOTOXIN DETECTION; 8.12 STATISTICAL ANALYSIS; 8.13 DETECTION OF PRIONS; 8.14 SUMMARY; REFERENCES; 9 Culture Collections and Biological Resource Centers (BRCs); 9.1 INTRODUCTION; 9.2 CULTURE COLLECTION FUNDING; 9.3 OPERATION; 9.4 QUALITY MANAGEMENT; 9.5 SERVICES; 9.6 SUMMARY; REFERENCES; FURTHER READING; 10 Culture Preservation; 10.1 INTRODUCTION; 10.2 CULTURE AND PRESERVATION OF BACTERIA; 10.3 CULTURE AND PRESERVATION OF FUNGI AND YEAST; 10.4 CULTURE AND PRESERVATION OF CELL CULTURES; REFERENCES

11 Expression and Secretion of Heterologous Proteins, Bacillus and Other Gram-Positive Bacteria

Sommario/riassunto

Biotechnology represents a major area of research focus, and many universities are developing academic programs in the field. This guide to biomanufacturing contains carefully selected articles from Wiley's Encyclopedia of Industrial Biotechnology, Bioprocess, Bioseparation, and Cell Technology as well as new articles (80 in all,) and features the same breadth and quality of coverage and clarity of presentation found in the original. For instructors, advanced students, and those involved in regulatory compliance, this two-volume desk reference offers an accessible and comprehensive reso