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Record Nr. |
UNINA9910954876203321 |
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Autore |
Xia Wenshui |
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Titolo |
Chitosan hydrolysis by non-specific enzymes / / Wenshui Xia and Ping Liu |
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Pubbl/distr/stampa |
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New York, : Nova Science Publishers, c2010 |
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ISBN |
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Edizione |
[1st ed.] |
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Descrizione fisica |
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1 online resource (78 p.) |
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Collana |
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Environmental Science, Engineering and Technology |
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Altri autori (Persone) |
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Disciplina |
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Soggetti |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Intro -- CHITOSAN HYDROLYSIS BY NON-SPECIFIC ENZYMES -- CHITOSAN HYDROLYSIS BY NON-SPECIFIC ENZYMES -- CONTENTS -- ABSTRACT -- Chapter 1 INTRODUCTION -- Chapter 2 CHARACTERIZATION OF CHITOSAN HYDROLYSIS BY NONSPECIFIC ENZYMES -- 2.1. CHARACTERIZATION OFCHITOSAN HYDROLYSIS BY NON-SPECIFIC CELLULASES -- (1) Temperature and pH -- (2) Molecular Weight -- (3) Reduction of the Viscosity -- (4) Kinetic Parameters -- (5) Metal Ions -- 2.2. CHARACTERIZATION OF CHITOSAN HYDROLYSIS BY NON-SPECIFIC LIPASE -- 2.3. Characterization of Chitosan Hydrolysis by Non-Specific Papain -- Chapter 3 MECHANISM OF NON-SPECIFIC ENZYMES TOWARD CHITOSAN -- 3.1. PURIFICATION AND CHARACTERIZATION OF CHITOSANOLYTIC COMPONENTS FROM NON -SPECIFIC ENZYMES -- 3.1.1. Purification and Characterization of a Bifunctional Enzyme with Chitosanolytic and Cellulolytic Activity from Commercial Cellulase -- 3.1.2. Purification and Characterization of a Bifunctional Chitosanase with Chitinase Activity from Commercial Lipase -- 3.2. ACTION MODE ANALYSIS OF BIFUNCTIONAL ENZYMES ON CHITOSAN -- 3.2.1. Substrate Specificity -- 3.2.2. Chitosan and Chitooligosaccharide Hydrolysis Analysis by CCBE -- 3.2.3. TLC Analysis of COS Hydrolysis Products by the Purified CNBE -- Chapter 4 STRUCTURE AND FUNCTION ANALYSIS OF BIFUNCTIONAL ENZYMES WITH CHITOSANOLYTIC ACTIVITY -- 4.1. AMINO ACID COMPOSITION OF PURIFIED CCBE AND CNBE -- 4.2. STRUCTURE ANALYSIS OF PURIFIED CCBE AND CNBE -- 4.2.1. N-Terminal Sequencing Analysis of CNBE -- |
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4.2.2. Sequencing Analysis of the Purified CCBE by MALDI-TOF Mass -- 4.3. DETERMINATION OF THE ACTIVE SITES OF CCBE AND CNBE BY CHEMICAL MODIFICATION -- 4.3.1. The Essential Carboxyl Group Modification using EDC -- 4.3.2. Modification of Tryptophan Residues using NBS -- 4.3.3. Modification of Histidine Residue using DEPC. |
4.3.4. Modification of Tyrosine, Threonine/Serine, Arginine, Methionine Residues Etc -- Chapter 5 IDENTIFICATION OF CCBE FROM T.VIRIDE BY MOLECULAR CLONING -- 5.1. DETERMINATION OF FERMENTATION CONDITION OF T.VIRIDE -- 5.2. CLONING AND SEQUENCE ANALYSIS OF CCBE GENE -- 5.3. EXPRESSION ANALYSIS OF CCBE SPLICING GENES IN PICHIA PATORIS -- ACKNOWLEDGMENTS -- REFERENCE -- INDEX -- Blank Page. |
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Sommario/riassunto |
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The focus of this book is the characterizations and hydrolyzing mechanism of the non-specific enzymes toward chitosan choosing the three typical non-specific enzymes: cellulase, lipase and papain as objects. The authors studied the enzymatic characteristics, purification, product analysis, glycoside bond cleavage, active sites and gene cloning of these enzymes to expatiate their non-specific hydrolysis mechanism. |
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