1.

Record Nr.

UNINA9910877616103321

Titolo

Molecular biology problem solver : a laboratory guide / / edited by Alan S. Gerstein

Pubbl/distr/stampa

New York, : Wiley, c2001

ISBN

1-280-36659-1

9786610366590

0-470-24459-3

0-471-46103-2

0-471-22390-5

Descrizione fisica

1 online resource (591 p.)

Altri autori (Persone)

GersteinAlan S. <1957->

Disciplina

572.8

572.8078

Soggetti

Molecular biology - Methodology

Molecular biology

Lingua di pubblicazione

Inglese

Formato

Materiale a stampa

Livello bibliografico

Monografia

Note generali

Description based upon print version of record.

Nota di bibliografia

Includes bibliographical references and index.

Nota di contenuto

MOLECULAR BIOLOGY PROBLEM SOLVER; Contents; Preface; Contributors; Chapter 1. Preparing for Success in the Laboratory; Chapter 2. Getting What You Need from a Supplier; Chapter 3. The Preparation of Buffers and Other Solutions: A Chemist's Perspective; Chapter 4. How to Properly Use and Maintain Laboratory Equipment; Chapter 5. Working Safely with Biological Samples; Chapter 6. Working Safely with Radioactive Materials; Chapter 7. DNA Purification; Chapter 8. RNA Purification; Chapter 9. Restriction Endonucleases; Chapter 10. Nucleotides, Oligonucleotides, and Polynucleotides; Chapter 11. PCR

Chapter 12. ElectrophoresisChapter 13. Western Blotting; Chapter 14. Nucleic Acid Hybridization; Chapter 15. E. coli Expression Systems; Chapter 16. Eukaryotic Expression; Index

Sommario/riassunto

Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. Molecular Biology Problem Solver: A



Laboratory Guide asks the reader to consider crucial questions, such as:Have you selected the most appropriate research strategy?Have you identified the issues critical to your successful application of a technique?Are you familiar