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Record Nr. |
UNINA9910876999303321 |
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Titolo |
Cell-free protein synthesis : methods and protocols / / edited by Alexander S. Spirin and James R. Swartz |
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Pubbl/distr/stampa |
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Weinheim, : Wiley-VCH, c2008 |
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ISBN |
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3-527-69150-2 |
1-281-94699-0 |
9786611946999 |
3-527-62270-5 |
3-527-62269-1 |
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Descrizione fisica |
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1 online resource (264 p.) |
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Altri autori (Persone) |
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SpirinA. S (Aleksandr Sergeevich) |
SwartzJames R |
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Disciplina |
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Soggetti |
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Proteins - Synthesis |
Genetic translation |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Cell-free Protein Synthesis; Contents; Preface; List of Contributors; 1 Cell-free Protein Synthesis Systems: Historical Landmarks, Classification, and General Methods; 1.1 Introduction: Historical Landmarks; 1.1.1 Discovery of Protein Synthesis in Cell Extracts; 1.1.2 Translation of Exogenous Messages; 1.1.3 Coupled Transcription-translation in Bacterial Extracts; 1.1.4 Combined Transcription-translation Systems; 1.1.5 Continuous Flow/Continuous Exchange Principle; 1.2 Prokaryotic and Eukaryotic Types of Cell-free Expression Systems; 1.2.1 Cell Extracts; 1.2.1.1 E. coli extract (ECE) |
1.2.1.2 Wheat Germ Extract (WGE)1.2.1.3 Rabbit Reticulocyte Lysate (RRL); 1.2.2 Genetic Constructs (Expression Vectors); 1.2.2.1 Prokaryotic Systems; 1.2.2.2 Eukaryotic Systems; 1.3 Preparing Cell Extracts; 1.3.1 E. coli Extracts; 1.3.1.1 Genetics; 1.3.1.2 Cell Growth; 1.3.1.3 Extract Preparation; 1.3.2 Wheat Germ Extracts; 1.4 Designing Reaction Composition; 1.4.1 Mg(2+) and Phosphate; 1.4.2 Other Salts; 1.4.3 Nucleotides and Amino Acids; 1.4.4 Stabilization Reagents; 1.4.5 Other Factors; 1.5 Providing Energy; 1.5.1 Direct Nucleotide |
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