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Record Nr. |
UNINA9910874678403321 |
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Autore |
Cohen William W |
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Titolo |
A Computer Scientist's Guide to Cell Biology |
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Pubbl/distr/stampa |
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Cham : , : Springer International Publishing AG, , 2024 |
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©2024 |
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ISBN |
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Edizione |
[2nd ed.] |
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Descrizione fisica |
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1 online resource (121 pages) |
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Altri autori (Persone) |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Nota di contenuto |
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Intro -- Acknowledgments -- Contents -- Introduction -- How Cells Work: The Basics -- What Life Is Made Of -- DNA -- Proteins -- Lipids and Membranes -- Types of Life -- Prokaryotes -- Eukaryotes -- Multicellular Life, Tissues, and Signaling -- Viruses -- Plasmids -- Prions -- Cellular Activity -- The Central Dogma -- Cellular Signaling -- Cell Division -- Why Is Biology Hard? -- Proteins Interact in Complexes and Pathways -- Individual Interactions Can Be Complicated -- Enzymes Control Reaction Rates -- Reaction Rates Can Be Highly Nonlinear -- Enzymatic Pathways Are Complicated -- Cellular Energy -- Enzymatic Pathways Have Many Steps -- Amplification and Pathways -- Modularity and Locality Is Limited -- How Things That Interact Find Each Other -- Membranes and Locality -- Transport by Vesicles and Along Microtubules -- Biological Processes Can Cross Membranes -- Receptors -- Ion Channels and Neural Signals: How Nerves Talk -- How Nerves Talk -- Wrap-Up -- Looking at Very Small Things -- Limitations of Optical Microscopes -- Fluorescent Microscopes -- Confocal Microscopes -- Electron Microscopes -- Manipulation of the Very Small -- Taking Small Things Apart -- Sorting Small Things -- Centrifugation: Separation by Weight -- Chromatography: Separation by Charge or Other Properties -- Electrophoresis: Separation by Size or Shape -- The Many Approaches to Sorting and Selection -- Measuring Proteins at Scale -- Microarrays and Gene Chips -- Parallelism, Automation, and Reuse in Biology -- Liquid-Handling Robots -- Databases of Prepared Samples -- |
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Classifying Things by Their Pieces -- Histograms and Peptide Maps -- Mass Spectrometry -- DNA Fingerprinting -- Wrap-Up on Classifying Things by Their Parts -- Reprogramming Cells -- Our Friends, the Microorganisms -- Restriction Enzymes and Restriction-Methylase Systems. |
Constructing Recombinant DNA with REs and DNA Ligase -- CRISPR/Cas9 -- Inserting Foreign DNA into a Cell -- Plasmids as Insertion Vectors -- Markers -- Promoters and Regulating Recombinant Organisms -- Phages as Insertion Vectors -- Using Genomic DNA Libraries -- Creating Novel Proteins: Tagging and Phage Display -- Yeast Two-Hybrid Assays Using Fusion Proteins -- Other Ways to Use Biology for Biological Experiments -- Replicating DNA in a Test Tube -- DNA Replication: The Basics -- DNA Replication: Not So Basic -- DNA Replication in a Tube: PCR -- Sequencing DNA by Partial Replication and Sorting -- Sanger Sequencing -- Massively Parallel Sequencing -- How Fast, Cheap Sequencing Has Changed Biology -- Other In Vitro Systems: Translation and Reverse Transcription -- Translation in a Tube -- Reverse Transcription -- Antibodies: Exploiting the Natural Defenses of a Cell -- Immunofluorescence -- Antibodies in Action: COVID Tests -- Costs and Benefits -- mRNA Vaccines -- Traditional Vaccines -- mRNA Vaccines -- RNA Interference -- Serial Analysis of Gene Expression -- Bioinformatics -- DNA Sequence Analysis -- Levenshtein Distance -- Smith-Waterman Similarity -- Multiple Sequence Alignment -- Analyzing Similarities of Species -- Molecular Clocks -- Data Mining DNA Sequence Databases -- High-Throughput Experiments -- Search Engines -- Where to Go From Here? -- What Now? -- Sources -- In preparing the first edition of the book, the textbooks and/or web sites below were used as references. For the second edition, we've updated our descriptions and added a few others. -- Index. |
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