The 10th day of Aprill 1655. A true report of the great number of poor children, and other poor people maintained in the severall hospitals by the pious care of the Lord Mayor, commonalty and citizens of the city of London [[electronic resource]]

• Record Nr.: UNISA996391418203316
• Pubbl/distr/stampa: [London, : s.n., 1655]
• Descrizione fisica: 1 sheet ([1] p.)
• Soggetti: Hospitals - Cost of operation; Hospitals - Great Britain; Poor - England - London
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Imprint from Wing.; A report on Christ's hospital, St. Bartholomew's, St. Thomas's, Bridewell, and Bethlem.; Reproduction of the original in the British Library.
• Sommario/riassunto: eebo-0018

Cooperative grain marketing : changes, issues, and alternatives / / Marc Warman

• Record Nr.: UNINA9910705181603321
• Autore: Warman Marc
• Pubbl/distr/stampa: Washington, D.C. : , : United States Department of Agriculture, Agricultural Cooperative Service, , 1994
• Descrizione fisica: 1 online resource (v, 18 pages) : illustrations
• Collana: ACS research report ; ; 123
• Soggetti: Grain - Cooperative marketing - United States; Grain - Cooperative marketing - Government policy - United States
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: "April 1994."; Title from title screen (viewed on Feb. 11, 2014).
• Nota di bibliografia: Includes bibliographical references (pages 17-18).

In situ hybridization [[electronic resource] /] / Melody Clark

• Record Nr.: UNINA9910830987403321
• Pubbl/distr/stampa: [Saddle River, NJ?, : Wiley-VCH, 2002?]
• ISBN: 1-281-75854-X; 9786611758547; 3-527-61507-5; 3-527-61506-7
• Descrizione fisica: 1 online resource (182 p.)
• Collana: Laboratory companion
• Altri autori (Persone): ClarkMelody
• Disciplina: 572.84; 574.87322
• Soggetti: In situ hybridization; Genetics
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: In Situ Hybridization; Contents; CHAPTER 1. Genomic In Situ Hybridization for Whole Chromosome and Genome Analysis; 1.1 Introduction; 1.2 Uses of Genomic In Situ Hybridization; 1.2.1 Genome Organization of Different Sequence Classes; 1.2.2 Species Identity, Differentiation and Relatedness; 1.2.3 Genomic Stability; 1.2.4 Spatial Organization of Chromosomes; 1.2.5 Chromosome lntrogression and Rearrangements; 1.2.6 Chromosome Behaviour and Meiosis; 1.3 The Principle of Genomic In Situ Hybridization; 1.3.1 Genomic Probing; 1.3.2 Blocking and Competitive Hybridization; 1.4 Materials and Chemicals; 1.4.1 Buffers and Reagents1.4.2 Chemicals; 1.4.3 Equipment; 1.5 Genomic In Situ Hybridization: Protocol; 1.5.1 Chromosome Preparations; 1.5.2 Probe DNA; 1.5.3 Blocking DNA; 1.5.4 In Situ Hybridization Procedure; 1.6 Troubleshooting; CHAPTER 2. Fluorescence In Situ Hybridization: Applications in Gene Mapping and Clinical Diagnostics; 2.1 Introduction; 2.2 Materials and Chemicals; 2.2.1 Buffers and Reagents; 2.2.2 Chemicals; 2.2.3 Equipment; 2.3 FISH Using Single Copy Cosmid Probes; 2.3.1 Preparation of Metaphase Chromosomes; 2.3.2 Preparation of Metaphase Chromosome Slides for ; Hybridization; 2.3.3 Preparation and Labelling of Probes2.3.4 Hybridization of Cosmid Probes; 2.4 Multicolor FISH Using Cosmid Probes; 2.4.1 Labelling of Probes and Hybridization Reactions; 2.5 lnterphase FISH Analysis; 2.5.1 Preparation of lnterphase Nuclear Slides from Short Term Cultures; 2.5.2 Direct Preparation of lnterphase Spreads; 2.5.3 FISH; 2.6 FISH Analysis Using YAC Clones; 2.6.1 Preparation of Metaphase Chromosomes; 2.6.2 Isolation of YAC DNA; 2.6.3 Biotin Labelling of YACs; 2.6.4 FISH; 2.6.5 Microscopy; 2.7 Direct Visual In Situ Hybridization (DIRVISH); 2.7.1 Preparation of Stretched DNA; 2.7.2 FISH Hybridization2.7.3 Detection and Analysis; 2.7.4 Microscopy; 2.8 Troubleshooting; CHAPTER 3. Detection of Nucleic Acids (DNA and RNA) In Situ by Single and Cyclic Primed In Situ labelling (PRINS): Two Alternatives to Traditional In Situ Hybridization Methods; 3.1 Introduction; 3.2 Materials and Chemicals; 3.2.1 Buffers and Reagents; 3.2.2 Chemicals; 3.2.3 Equipment; 3.3 DNA-PRINS with Oligonucleotide Probes; 3.4 PRINS with Ddel Digested Cloned Probes; 3.4.1 Generation of Primers from Cloned Alpha Satellite DNA; 3.4.2 PRlNS Reaction; 3.5 Multicolour-PRINS; 3.6 PRINS-painting; 3.7 PRINS-PCR and Repeated-PRINS of DNA3.7.1 Chromosome Spreads; 3.7.2 Pretreatment of Chromosome Spreads; 3.7.3 Labelling Reaction; 3.8 PRINS-PCR of mRNA; 3.9 Visualization of Hapten-Labelled Nucleotides; 3.9.1 Standard Slides; 3.9.1.1 Biotin; 3.9.1.2 Digoxigenin; 3.9.2 Micro-slides; 3.9.2.1 Detection of Labelled DNA; 3.9.2.2 Detection of Labelled RNA; 3.9.2.3 Alkaline Phosphatase Visualization; 3.9.2.4 Fluorescent Visualization; 3.10 Troubleshooting; CHAPTER 4. Fluorescence lmmunophenotyping and lnterphase Cytogenetics as a Tool for Investigation of Neoplasms (FICTION): Combined In Situ Hybridization and Fluorescence lmmunophenotyping
• Sommario/riassunto: In situ hybridization is a proven, powerful technique with applications in chromosome and genome analysis, as well as gene expression. Covering a carefully selected range of techniques with immediate and general applications in research and clinical diagnosis, the book starts with genome and DNA mapping, continues through gene expression localization in wholemount and tissue sections, and on to ultrastructural levels. The step-by-step protocols used reflect research in these areas and are all reproducible.