Single cell analysis [[electronic resource] ] : technologies and applications / / edited by Dario Anselmetti

• Record Nr.: UNINA9910830701603321
• Pubbl/distr/stampa: Weinheim, : Wiley-VCH, c2009
• ISBN: 1-282-46084-6; 9786612460845; 3-527-62665-4; 3-527-62664-6
• Descrizione fisica: 1 online resource (286 p.)
• Altri autori (Persone): AnselmettiD
• Disciplina: 571.6
• Soggetti: Cells - Analysis; Cytology
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Single Cell Analysis: Technologies and Applications; Contents; Foreword; Preface; List of Contributors; Part I Single Cell Analysis: Imaging; 1 Single Molecule Fluorescence Monitoring in Eukaryotic Cells: Intranuclear Dynamics of Splicing Factors; 1.1 Motivation; 1.2 Experimental Approach; 1.3 Single Particle Tracking within Living Cells; 1.4 Pre-Messenger RNA Splicing; 1.5 Intranuclear Splicing Factor Tracking; 1.6 Intranuclear U1 snRNP Splicing Factor Binding; 1.7 Events in Speckles; 1.8 Intranuclear U1 snRNP Mobility; 1.9 Perspectives of Single Molecule Microscopy; References; 2 Gene Classification and Quantitative Analysis of Gene Regulation in Bacteria using Single Cell Atomic Force Microscopy and Single Molecule Force Spectroscopy2.1 Introduction; 2.2 AFM on Paracrystalline Cell Surface Layers of C. glutamicum: Protein Sequence Information and Morphology; 2.3 Imaging of Living C. glutamicum Cells with Molecular Resolution: Genes, Transcriptional Regulation and Morphology; 2.4 Single Molecule Force Spectroscopy on Specific Protein-DNA Complexes: Transcriptional Regulation in S. meliloti; 2.5 Effector-Induced Protein-DNA Binding on the Single Molecule Level: Quorum Sensing in S. meliloti2.6 Conclusion; References; 3 Cellular ; Cryo-Electron Tomography (CET): Towards a Voyage to the Inner Space of Cells; 3.1 Introduction; 3.2 Tomography with the Electron Microscope - a Practical Perspective; 3.2.1 Sample Preparation; 3.2.2 Instrumental and Technical Requirements; 3.2.3 Alignment, Reconstruction and Visualization; 3.3 Molecular Interpretation of Cellular Tomograms; 3.4 Outlook: The Future is Bright; References; Part II Single Cell Analysis: Technologies; 4 Single Cell Proteomics; 4.1 Introduction4.2 The Challenge; 4.3 Single Cell Proteomics: Mass Spectrometry; 4.4 Single Cell Separations; 4.5 Ultrasensitive Protein Analysis: Capillary Electrophoresis with Laser-Induced Fluorescence Detection; 4.6 Capillary Sieving Electrophoresis of Proteins from a Single Cancer Cell; 4.7 Cell Cycle-dependent Single Cell Capillary Sieving Electrophoresis; 4.8 Tentative Identification of Proteins in Single Cell Electropherograms; 4.9 Capillary Micellar and Submicellar Separation of Proteins from a Single Cell; 4.10 Two-Dimensional Capillary Electrophoresis of Proteins in a Single Cell; 4.11 Single Copy Detection of Specific Proteins in Single Cells4.12 Conclusion; References; 5 Protein Analysis of Single Cells in Microfluidic Format; 5.1 Introduction; 5.2 Microfluidic Single Cell Analysis Concept; 5.2.1 Single Cell Selection and Trapping; 5.2.2 Single Cell Lysis; 5.3 Single Cell Electrophoretic Separation and Detection of Proteins; 5.3.1 Label-Based Fluorescence Detection; 5.3.2 Label-Free Fluorescence Detection; 5.3.2.1 UV-LIF in Quartz Microfluidic Devices; 5.3.2.2 UV-LIF in PDMS Microfluidic Devices; 5.3.2.3 Single Cell UV-LIF Electrophoretic Analysis; 5.4 Future Directions in Single Cell Analysis
• Sommario/riassunto: The first-ever comprehensive overview of the methods used in this key technology in modern biology provides the latest working knowledge needed by every scientist entering this growing field. It covers all the current technology and application areas, from microscopy and spectroscopy to proteomics and microfluidics.

Ecologies of the heart : emotion, belief, and the environment / / E.N. Anderson

• Record Nr.: UNINA9910972764703321
• Autore: Anderson Eugene Newton, Jr., <1941->
• Pubbl/distr/stampa: New York, : Oxford University Press, 1996
• ISBN: 0-19-756061-X; 1-280-52747-1; 0-19-535818-X; 1-4294-1548-7
• Edizione: [1st ed.]
• Descrizione fisica: 1 online resource (ix, 256 pages)
• Collana: Oxford scholarship online
• Disciplina: 333.717; 363.7; 574.5
• Soggetti: Environmental policy; Environmental protection; Human ecology; Environmental sciences - Philosophy
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Previously issued in print: 1996.
• Nota di bibliografia: Includes bibliographical references (p. [215]-233) and index.
• Nota di contenuto: Contents; 1. Landscape with Figures; 2. Feng-shui: Ideology and Ecology; 3. Chinese Nutritional Therapy; 4. Learning from the Land Otter: Religious Representation of Traditional Resource Management; 5. Managing the Rainforest: Maya Agriculture in the Town of the Wild Plums; 6. Needs and Human Nature; 7. Information Processing: Rational and Irrational Transcended; 8. Culture: Ecology in a Wider Context; 9. In and Out of Institutions; 10. The Disenchanted: Religion as Ecological Control, and Its Modern Fate; 11. A Summary, and Some Suggestions; Notes; References; Index
• Sommario/riassunto: In Ecologies of the Heart, Anderson reveals how religion and other folk beliefs helped pre-industrial peoples control and protect their resources. He also presents a coherent view of long-term, sustainable policies for the environment.