| |
|
|
|
|
|
|
|
|
1. |
Record Nr. |
UNINA9910324037103321 |
|
|
Autore |
Gosseries Axel |
|
|
Titolo |
Entre ville et nature, les sites semi-naturels / / François Ost, Jean Remy, Luc Van Campenhoudt |
|
|
|
|
|
|
|
Pubbl/distr/stampa |
|
|
Bruxelles, : Presses de l’Université Saint-Louis, 2019 |
|
|
|
|
|
|
|
ISBN |
|
|
|
|
|
|
Descrizione fisica |
|
1 online resource (299 p.) |
|
|
|
|
|
|
Altri autori (Persone) |
|
OstFrançois |
RemyJean |
SadeleerNicolas de |
SambonJacques |
SchautChristine |
Van CampenhoudtLuc |
Van CampenhoudtLuc |
|
|
|
|
|
|
|
|
Disciplina |
|
|
|
|
|
|
Soggetti |
|
Natural areas - Belgium |
Open spaces - Belgium |
|
|
|
|
|
|
|
|
Lingua di pubblicazione |
|
|
|
|
|
|
Formato |
Materiale a stampa |
|
|
|
|
|
Livello bibliografico |
Monografia |
|
|
|
|
|
Sommario/riassunto |
|
On connaissait déjà, de longue date, les parcs et les espaces verts : soigneusement aménagés et jalousement gardés, ils rompent la monotonie de l'espace bâti en y introduisant une image de nature « civilisée ». En revanche, on redécouvre seulement aujourd'hui, à la lisière de nos cités, les sites semi-naturels, qui, parce qu'ils ont été le plus souvent laissés à l'abandon, nous livrent quelque chose de la nature « naturelle ». Les biologistes ont mis en lumière la richesse de leur écosystème, la variété de leur faune et de leur flore. C'est cependant à une autre forme de « défense et d'illustration » que s'attache cet ouvrage. Au-delà de leur diversité biologique, il restait en effet à faire apparaître leur valeur sociale : une analyse sociologique approfondie s'y emploie qui met en lumière les multiples images que s'en font les habitants de la ville, ainsi que les divers usages qui s'y développent. Ressources biologiques uniques en milieu urbain, et |
|
|
|
|
|
|
|
|
|
|
|
|
|
enjeux d'usages tantôt complémentaires, tantôt opposés, les sites semi-naturels appellent une gestion démocratique et un traitement juridique protecteur. C'est à l'étude des virtualités des instruments juridiques disponibles que s'attache la seconde partie de l'ouvrage. Un cadre est ainsi dressé en vue d'assurer une protection réelle de ces espaces-lisières qui occupent d'ores et déjà une place de choix dans le patrimoine commun des citadins. Six sites bruxellois ont été choisis en raison de leur valeur illustrative : le Kauwberg, le Scheutbos, le Moeraske, le Neerpede, le Kattebroeck, ainsi que le plateau de la Foresterie. Ces deux études ont été menées à l'initiative du fonds de l'environnement de la fondation Roi Baudouin. |
|
|
|
|
|
|
2. |
Record Nr. |
UNINA9910830665603321 |
|
|
Autore |
Kellner R. |
|
|
Titolo |
Microcharacterization of proteins / / R. Kellner, F. Lottspeich, H. E. Meyer |
|
|
|
|
|
|
|
Pubbl/distr/stampa |
|
|
Weinheim, Germany : , : WILEY-VCH, , 1999 |
|
©1999 |
|
|
|
|
|
|
|
|
|
ISBN |
|
1-281-84250-8 |
9786611842505 |
3-527-61396-X |
3-527-61397-8 |
|
|
|
|
|
|
|
|
Edizione |
[2nd ed.] |
|
|
|
|
|
Descrizione fisica |
|
1 online resource (349 p.) |
|
|
|
|
|
|
Disciplina |
|
|
|
|
|
|
|
|
Soggetti |
|
Proteins - Analysis - Methodology |
Peptides - Analysis - Methodology |
Amino acids - Analysis - Methodology |
|
|
|
|
|
|
|
|
Lingua di pubblicazione |
|
|
|
|
|
|
Formato |
Materiale a stampa |
|
|
|
|
|
Livello bibliografico |
Monografia |
|
|
|
|
|
Note generali |
|
Description based upon print version of record. |
|
|
|
|
|
|
Nota di bibliografia |
|
Includes bibliographical references at the end of each chapters and index. |
|
|
|
|
|
|
|
|
Nota di contenuto |
|
Microcharacterization of Proteins; Contents; Section I: Overview; I.1 Microcharacterization of Proteins; 1 General Aspects; 2 From a Cell to a |
|
|
|
|
|
|
|
|
|
|
|
Protein Sequence; 3 Genome and Proteome; Section II: Microseparation Techniques and Sample Preparation; II.1 Microseparation Techniques I: High Resolution Gel-Electrophoretic Techniques : Qualitative, Quantitative and Micropreparative Applications; 1 Introduction; 2 Theory; 3 Media and Equipment; 3.1 Media; 3.2 Equipment; 4 Gel Electrophoretic Methods; 4.1 Disc Electrophoresis; 4.2 Gradient Gel Electrophoresis; 4.3 Additives in Electrophoresis |
4.4 SDS Electrophoresis4.5 Isoelectric Focusing; 4.6 Two-Dimensional Electrophoresis (2 -DE); 5 References; II.2 Microseparation Techniques II: Gel Electrophoresis for Sample Preparation in Protein Chemistry; 1 Introduction; 2 Denaturing Techniques; 2.1 Commonly Used SDS-Polyacrylamide Gel Electrophoresis Techniques for Protein Separation; 2.2 Blue-SDS-PAGE for Recovery of Membrane Proteins from Gels; 2.3 Electroelution of Proteins after Blue-SDS-PAGE; 2.4 Electroblotting of Blue and Colorless SDS Gels; 2.5 Isoelectric Focusing in the Presence of Urea; 3 Native Techniques |
3.1 Colorless-Native-PAGE3.2 Blue-Native-PAGE; 3.3 Native Isoelectric Focusing; 4 References; II.3 Microseparation Techniques III: Electroblotting; 1 Introduction; 2 Electroblotting; 2.1 Polyacrylamide Gel Electrophoresis; 2.2 Blot Systems; 2.2.1 Tank Blotting; 2.2.2 Semidiy Blotting; 2.3 Blotting Parameters; 2.3.1 The Blotting Process; 2.3.2 Transfer Buffers; 2.3.3 Addition of SDS; 2.3.4 Addition of Methanol; 2.3.5 Influence of Protein Concentration; 3 Blotting Membranes; 4 References; II.4 Microseparation Techniques IV: Analysis of Peptides and Proteins by Capillary Electrophoresis |
1 Introduction2 Theory; 2.1 Capillary Isotachophoresis; 2.2 Capillary Zone Electrophoresis; 2.3 Electroosmotic Flow; 3 Instrumentation; 3.1 Injection; 3.2 Detection; 4 Applications; 4.1 Peptide Separations; 4.2 Protein Separations; 5 References; II.5 Microseparation Techniques V: High Performance Liquid Chromatography; 1 Introduction; 2 Principle of HPLC; 3 Getting Started; 3.1 Solvents; 3.2 Pump; 3.3 Gradient; 3.4 Pre-Column Split; 3.5 Sample Preparation; 3.6 Injector; 3.7 Tubings; 3.8 In-Line Filter, Guard Column; 3.9 Column; 3.10 Detection; 3.11 Fractionation; 4 Applications; 5 References |
II.6 Sample Preparation I: Removal of Salts and Detergents1 Introduction; 2 Removal of Salts or Polar Components; 2.1 Protein-Binding Membranes; 2.2 High Performance Liquid Chromatography; 2.3 Desalting on Microcolumns; 3 Removal of Detergents and Apolar Contaminants; 3.1 Detergents; 3.2 Protein-binding Membranes; 3.3 Precipitation; 3.4 SDS-PAGE; 3.5 Concentrating Gels; 4 "Golden Rules" for Protein and Peptide Handling; 5 References; II.7 Sample Preparation II: Chemical and Enzymatic Fragmentation of Proteins; 1 Strategy; 2 Denaturation, Reduction and Alkylation; 3 Enzymatic Fragmentation |
3.1 Enzymes |
|
|
|
|
|
|
Sommario/riassunto |
|
Proteomics - the analysis of the whole set of proteins and their functions in a cell - is based on the revolutionary developments which have been achieved in protein analysis during the last years. The number of finished genome projects is growing and in parallel there is a dramatically increasing need to identify the products of revealed genes. Acting on a micro level modern protein chemistry increases our understanding of biological events by elucidating the relevant structure-function relationships. The second edition of the successful title Microcharacterization of Proteins present |
|
|
|
|
|
|
|
| |