| |
|
|
|
|
|
|
|
|
1. |
Record Nr. |
UNINA9910829987003321 |
|
|
Titolo |
Photoproteins in bioanalysis [[electronic resource] /] / edited by Sylvia Daunert and Sapna K. Deo |
|
|
|
|
|
|
|
Pubbl/distr/stampa |
|
|
Weinheim, : Wiley-VCH, c2006 |
|
|
|
|
|
|
|
ISBN |
|
1-280-72271-1 |
9786610722716 |
3-527-60914-8 |
3-527-60869-9 |
|
|
|
|
|
|
|
|
Descrizione fisica |
|
1 online resource (258 p.) |
|
|
|
|
|
|
Altri autori (Persone) |
|
|
|
|
|
|
|
|
Disciplina |
|
|
|
|
|
|
Soggetti |
|
Proteins |
Biomolecules - Analysis |
Biochemistry - Technique |
Photoluminescence |
Bioluminescence |
|
|
|
|
|
|
|
|
Lingua di pubblicazione |
|
|
|
|
|
|
Formato |
Materiale a stampa |
|
|
|
|
|
Livello bibliografico |
Monografia |
|
|
|
|
|
Note generali |
|
Description based upon print version of record. |
|
|
|
|
|
|
Nota di bibliografia |
|
Includes bibliographical references and index. |
|
|
|
|
|
|
Nota di contenuto |
|
Photoproteins in Bioanalysis; Contents; Preface; List of Contributors; 1 The Photoproteins; 1.1 Discovery of Photoprotein; 1.2 Various Types of Photoproteins Presently Known; 1.2.1 Radiolarian (Protozoa) Photoproteins; 1.2.2 Coelenterate Photoproteins; 1.2.3 Ctenophore Photoproteins; 1.2.4 Pholasin (Pholas Luciferin); 1.2.5 Chaetopterus Photoprotein; 1.2.6 Polynoidin; 1.2.7 Symplectin; 1.2.8 Luminodesmus Photoprotein; 1.2.9 Ophiopsila Photoprotein; 1.3 Basic Strategy of Extracting and Purifying Photoproteins; 1.4 The Photoprotein Aequorin; 1.4.1 Extraction and Purification of Aequorin |
1.4.1.1 Hydrophobic Interaction Chromatography1.4.2 Properties of Aequorin; 1.4.2.1 Stability; 1.4.2.2 Freeze-drying; 1.4.3 Specificity to Ca(2+); 1.4.4 Luminescence of Aequorin by Substances Other Than Divalent Cations; 1.4.5 Mechanism of Aequorin Luminescence and Regeneration of Aequorin; 1.4.5.1 Structure of Aequorin; 1.4.5.2 Luminescence Reaction; 1.4.5.3 Regeneration; 1.4.6 Inhibitors of |
|
|
|
|
|
|
|
|
|
|
|
Aequorin Luminescence; 1.4.7 Recombinant Aequorin; 1.4.8 Semi-synthetic Aequorins; 1.4.8.1 e-Aequorins; References; 2 Luminous Marine Organisms; 2.1 Introduction; 2.1.1 Non-luminous Taxa |
2.1.2 Luminous Taxa2.2 Taxonomic Distribution of Bioluminescence; 2.2.1 Bacterial Luminescence; 2.2.2 Dinoflagellate Luciferin; 2.2.3 Cypridina (Vargula) Luciferin; 2.2.4 Coelenterazine; 2.2.5 Other Luciferins: Known and Unknown; 2.3 Functions; 2.3.1 Startle or Distract; 2.3.2 Burglar Alarm; 2.3.3 Counterillumination; 2.3.4 Mating Displays; 2.3.5 Prey Attraction; References; 3 Beetle Luciferases: Colorful Lights on Biological Processes and Diseases; 3.1 Introduction; 3.2 Beetle Luciferases; 3.3 Bioanalytical Assays of ATP; 3.3.1 Biomass Estimation and Microbiological Contamination |
3.3.2 Cytotoxicity and Cell Viability Tests3.3.3 Enzymatic Assays; 3.4 Luciferases as Reporter Genes; 3.4.1 Dual and Multiple Reporter Assays; 3.5 Biophotonic Imaging in Animals: A Living Light on Diseases; 3.5.1 Pathogen Infection in Living Models; 3.5.2 Drug Screening; 3.5.3 Tumor Proliferation and Regression Studies; 3.5.4 Gene Delivery and Gene Therapy; 3.5.5 Luciferase as Biomarkers for Cell Trafficking Studies; 3.5.6 Immunoassays; 3.6 Biophotonic Imaging in Plants; 3.7 Biosensors: Sensing the Environment; 3.8 Novel Luciferases: Different Colors for Different Occasions; References |
4 Split Luciferase Systems for Detecting Protein-Protein Interactions in Mammalian Cells Based on Protein Splicing and Protein Complementation4.1 Introduction; 4.2 Protein Splicing-based Split Firefly Luciferase System [23]; 4.2.1 Split Luciferase Works as a Probe for Protein Interaction; 4.3 Split Renilla Luciferase Complementation System [33]; 4.3.1 Time Course of the Interaction Between Y941 and SH2n; 4.3.2 Location of the Interaction Between Y941 and SH2n; References; 5 Photoproteins in Nucleic Acid Analysis; 5.1 Hybridization Assays; 5.2 Quantitative Polymerase Chain Reaction |
5.3 Genotyping of Single-nucleotide Polymorphisms |
|
|
|
|
|
|
Sommario/riassunto |
|
The use of light-emitting proteins for the detection of biomolecules provides fast and sensitive methods which overcome the disadvantages of radioactive labels and the high cost of fluorescent dyes.This reference work summarizes modern advanced techniques and their applications and includes practical examples of assays based on photoproteins. The book presents contemporary key topics like luminescent marine organisms, DNA probes, reporter gene assays and photoproteins, ratiometric sensing, use of photoproteins for in vivo functional imaging and luminescent proteins in binding assays, to na |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
2. |
Record Nr. |
UNINA9910966984303321 |
|
|
Autore |
Crain Caleb |
|
|
Titolo |
American sympathy : men, friendship, and literature in the new nation / / Caleb Crain |
|
|
|
|
|
|
|
Pubbl/distr/stampa |
|
|
New Haven, : Yale University Press, c2001 |
|
|
|
|
|
|
|
ISBN |
|
9786611722845 |
9781281722843 |
1281722847 |
9780300133677 |
0300133677 |
|
|
|
|
|
|
|
|
Edizione |
[1st ed.] |
|
|
|
|
|
Descrizione fisica |
|
1 online resource (1 online resource (x, 310 p.) ) : ill |
|
|
|
|
|
|
Disciplina |
|
|
|
|
|
|
Soggetti |
|
American literature - Male authors - History and criticism |
Men in literature |
American literature - 19th century - History and criticism |
American literature - 1783-1850 - History and criticism |
Male friendship - United States - History |
Male friendship in literature |
Sympathy in literature |
|
|
|
|
|
|
|
|
Lingua di pubblicazione |
|
|
|
|
|
|
Formato |
Materiale a stampa |
|
|
|
|
|
Livello bibliografico |
Monografia |
|
|
|
|
|
Note generali |
|
Bibliographic Level Mode of Issuance: Monograph |
|
|
|
|
|
|
Nota di bibliografia |
|
Includes bibliographical references (p. 271-305) and index. |
|
|
|
|
|
|
Nota di contenuto |
|
In the pear grove : the romance of Leander, Lorenzo, and Castalio -- The decomposition of Charles Brockden Brown : sympathy in Brown's letters -- The transformation, the self devoted, and the dead recalled : sympathy in Brown's fiction -- The unacknowledged tie : young Emerson and the love of men -- Too good to be believed : Emerson's "Friendship" and the Samaritans -- The heart ruled out : Melville's Palinode. |
|
|
|
|
|
|
|
|
Sommario/riassunto |
|
"A friend in history," Henry David Thoreau once wrote, "looks like some premature soul." And in the history of friendship in early America, Caleb Crain sees the soul of the nation's literature. In a sensitive analysis that weaves together literary criticism and historical narrative, Crain describes the strong friendships between men that supported |
|
|
|
|
|
|
|
|
|
|
and inspired some of America's greatest writing--the Gothic novels of Charles Brockden Brown, the essays of Ralph Waldo Emerson, and the novels of Herman Melville. He traces the genealogy of these friendships through a series of stories. A dapper English spy inspires a Quaker boy to run away from home. Three Philadelphia gentlemen conduct a romance through diaries and letters in the 1780's. Flighty teenager Charles Brockden Brown metamorphoses into a horror novelist by treating his friends as his literary guinea pigs. Emerson exchanges glances with a Harvard classmate but sacrifices his crush on the altar of literature--a decision Margaret Fuller invites him to reconsider two decades later. Throughout this engaging book, Crain demonstrates the many ways in which the struggle to commit feelings to paper informed the shape and texture of American literature. |
|
|
|
|
|
| |