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1. |
Record Nr. |
UNINA990005825040403321 |
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Autore |
Savinio, Alberto <1891-1952> |
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Titolo |
Partita rimandata : diario calabrese (1948) / Alberto Savinio ; a cura di Vittorio Cappelli ; prefazione di Giuseppe Leonelli |
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Pubbl/distr/stampa |
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ISBN |
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Descrizione fisica |
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Collana |
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Disciplina |
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Locazione |
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Collocazione |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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2. |
Record Nr. |
UNINA990002069660403321 |
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Autore |
Buxton, Patrick A. |
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Titolo |
Animal life in deserts : a study of the fauna in relation to the environment / P. A. Buxton |
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Pubbl/distr/stampa |
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London : Edward Arnold, 1923 |
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Descrizione fisica |
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Locazione |
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Collocazione |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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3. |
Record Nr. |
UNINA9910829015203321 |
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Autore |
Westermeier Reiner |
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Titolo |
Electrophoresis in practice : a guide to methods and applications of DNA and protein separations / / Reiner Westermeier |
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Pubbl/distr/stampa |
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Weinheim, Germany : , : Wiley-VCH Verlag, , 2016 |
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ISBN |
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3-527-69516-8 |
3-527-69518-4 |
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Edizione |
[Fifth edition.] |
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Descrizione fisica |
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1 online resource (532 pages) |
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Classificazione |
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Disciplina |
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Soggetti |
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Electrophoresis |
DNA - Separation |
Proteins - Separation |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Includes bibliographical references and index |
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Nota di bibliografia |
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Includes bibliographical references at the end of each chapters and index. |
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Nota di contenuto |
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Title Page; Copyright; Table of Contents; Foreword; Abbreviations, |
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Symbols, Units; Preface; German Version; English Version, Fifth Edition; Part I: Fundamentals; Chapter 1: Electrophoresis; 1.1 General; 1.2 Electrophoresis in Nonrestrictive Gels; 1.3 Electrophoresis in Restrictive Gels; References; Chapter 2: Isotachophoresis; 2.1 Migration with the Same Speed; 2.2 "Ion Train" Separation; 2.3 Zone Sharpening Effect; 2.4 Concentration Regulation Effect; 2.5 Quantitative Analysis; References; Chapter 3: Isoelectric Focusing; 3.1 Principles; 3.2 Gels for IEF; 3.3 Temperature |
3.4 Controlling the pH Gradient; 3.5 Kinds of pH Gradients; 3.6 Protein Detection in IEF Gels; 3.7 Preparative Isoelectric Focusing; 3.8 Titration Curve Analysis; References; Chapter 4: High-Resolution Two-Dimensional Electrophoresis; 4.1 IEF in Immobilized pH Gradient Strips; 4.2 SDS-PAGE; 4.3 Proteomics; References; Chapter 5: Protein Sample Preparation; 5.1 Protein Quantification Methods; 5.2 Preparation of Native Samples; 5.3 Samples for SDS Electrophoresis; 5.4 Samples for High-Resolution 2D PAGE; References; Chapter 6: Protein Detection; 6.1 Fixation; 6.2 Poststaining Methods |
6.3 Prelabeling; 6.4 Difference Gel Electrophoresis (DIGE); 6.5 Imaging, Image Analysis, Spot Picking; References; Chapter 7: Blotting; 7.1 Transfer Methods; 7.2 Blotting Membranes; 7.3 Buffers for Electrophoretic Transfers; 7.4 General Staining; 7.5 Blocking; 7.6 Specific Detection; 7.7 Protein Sequencing; 7.8 Transfer Issues; 7.9 Electro-Elution of Proteins from Gels; References; Part II: Equipment and Methods; Chapter 8: Special Laboratory Equipment; Chapter 9: Consumables; Chapter 10: Chemicals; 10.1 Reagents; Method 1: PAGE of Dyes; M1.1 Sample Preparation; M1.2 Stock Solutions |
M1.3 Preparing the Casting Cassette; M1.4 Casting Ultra-Thin-Layer Gels; M1.5 Electrophoretic Separation; Method 2: Agarose and Immunoelectrophoresis; M2.1 Sample Preparation; M2.2 Stock Solutions; M2.3 Preparing the Gels; M2.4 Electrophoresis; M2.5 Protein Detection; References; Method 3: Titration Curve Analysis; M3.1 Sample Preparation; M3.2 Stock Solutions; M3.3 Preparing the Blank Gels; M3.4 Titration Curve Analysis; M3.5 Coomassie and Silver Staining; M3.6 Interpreting the Curves; References; Method 4: Native PAGE in Amphoteric-Buffers; M4.1 Sample Preparation; M4.2 Stock Solutions |
M4.3 Preparing the Empty Gels; M4.4 Electrophoresis; M4.5 Coomassie and Silver Staining; References; Method 5: Agarose IEF; M5.1 Sample Preparation; M5.2 Preparing the Agarose Gel; M5.3 Isoelectric Focusing; M5.4 Protein Detection; References; Method 6: PAGIEF in Rehydrated Gels; M6.1 Sample Preparation; M6.2 Stock Solutions; M6.3 Preparing the Blank Gels; M6.4 Isoelectric Focusing; M6.5 Coomassie and Silver Staining; M6.6 Perspectives; References; Method 7: Horizontal SDS-PAGE; M7.1 Sample Preparation; M7.2 Prelabeling with Fluorescent Dye; M7.3 Stock Solutions for Gel Preparation; M7.4 Preparing the Casting Cassette |
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