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Record Nr. |
UNINA9910828752503321 |
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Autore |
Kirby Lorne T. <1938-> |
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Titolo |
DNA fingerprinting : an introduction / / Lorne T. Kirby |
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Pubbl/distr/stampa |
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New York, : W.H. Freeman, 1992 |
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ISBN |
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0-19-756122-5 |
1-280-47118-2 |
9786610471188 |
1-60256-264-4 |
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Edizione |
[1st ed.] |
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Descrizione fisica |
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1 online resource (382 pages) |
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Disciplina |
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Soggetti |
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DNA fingerprinting |
Forensic genetics - Technique |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Originally published: New York : Stockton Press, c1990. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Intro -- CONTENTS -- 1. INTRODUCTION -- DEFINITION -- HISTORY -- A CASE FOR DNA -- APPLICATIONS -- REFERENCES -- 2. GENETIC PRINCIPLES -- DNA STRUCTURE -- Nuclear DNA -- Organelle DNA -- DNA FUNCTION -- REPRODUCTION -- LINKAGE -- PEDIGREE ANALYSIS -- MUTATION -- RESTRICTION ENDONUCLEASES -- CONCEPT OF POLYMORPHISM -- RECOMBINANT DNA -- BASE SEQUENCE DETERMINATION -- OLIGONUCLEOTIDE SYNTHESIS -- REPETITIVE SEQUENCE ORGANIZATION -- NOMENCLATURE OF ARBITRARY DNA SEGMENTS -- REFERENCES -- 3. LABORATORY ORGANIZATION -- LABORATORY SECTIONS -- Office -- Specimen Documentation -- Specimen Processing -- General Preparation -- Vector Handling -- Amplification by PCR -- Hybridization and Radioisotope Handling -- Darkroom -- Result Interpretation -- Tissue Culture -- Wash-up Area -- EQUIPMENT SUMMARY -- OPERATION -- Personnel -- Work Flow -- Safety -- REFERENCES -- 4. SPECIMENS -- COLLECTION -- STORAGE AND TRANSPORT -- DNA EXTRACTION -- Principles -- Automated System -- Manual System Organic Solvent Extraction -- Manual System Nonorganic Solvent Extraction -- DIALYSIS -- RNAASE TREATMENT -- QUANTITATION -- DNA CONCENTRATION -- QUALITY DETERMINATION -- EFFECTS OF ENVIRONMENTAL FACTORS INCLUDING |
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