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Record Nr. |
UNINA9910828394903321 |
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Titolo |
BSL3 and BSL4 agents : proteomics, glycomics, and antigenicity / / edited by Jiri Stulik ... [et al.] |
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Pubbl/distr/stampa |
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Weinheim, : Wiley-Blackwell, c2011 |
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ISBN |
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9781283835350 |
1283835355 |
9783527638208 |
3527638202 |
9783527638215 |
3527638210 |
9783527638192 |
3527638199 |
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Edizione |
[1st ed.] |
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Descrizione fisica |
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1 online resource (258 p.) |
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Altri autori (Persone) |
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Disciplina |
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Soggetti |
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Pathogenic microorganisms - Analysis |
Proteomics |
Glycomics |
Antigens |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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BSL3 and BSL4 Agents: Proteomics, Glycomics, and Antigenicity; Contents; Preface; List of Contributors; 1: Introduction: Application of Proteomic Technologies for the Analysis of Microbial Infections; 1.1 Introduction; 1.2 Search for New Factors of Virulence and Potential Diagnostic Markers; 1.3 Search for New Vaccine Candidates; 1.4 Analysis of Post-Translational Modifications of Bacterial Proteins and Protein-Protein Interactions; 1.5 Conclusions; References; Part One: Basic Proteomic Methods; 2: Separation of Proteins and Peptides; 2.1 Introduction; 2.1.1 Gel-Based Separation |
2.1.1.1 One-Dimensional Electrophoresis2.1.1.2 Two-Dimensional Electrophoresis; 2.1.1.3 Protein Staining and Image Analysis; 2.1.1.4 2-DE Limitations; 2.1.2 In Solution-"Gel Free" Proteomics; 2.1.3 Column |
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Chromatography; 2.1.3.1 Size Exclusion Chromatography; 2.1.3.2 Reversed-Phase Liquid Chromatography; 2.1.3.3 Hydrophilic Interaction Liquid Chromatography; 2.1.3.4 Ion Exchanger Chromatography; 2.1.3.5 Affinity Chromatography; 2.1.3.6 Multidimensional Chromatography; 2.1.4 Liquid Phase IEF and Electrophoresis; 2.1.5 Alternative Separation Technologies; Acknowledgment; References |
3: Basic Mass Spectrometric Approaches3.1 Introduction; 3.2 Ionization; 3.2.1 Matrix-Assisted Laser Desorption/Ionization; 3.2.2 Electrospray Ionization; 3.3 Mass Analyzers; 3.3.1 Time of Flight; 3.3.2 Reflectron TOF; 3.3.3 Quadrupole and Ion Trap; 3.3.4 Fourier Transformation Ion Cyclotron; 3.3.5 Tandem Mass Analyzers; 3.3.6 Ion Detection; 3.4 Protein Identification; 3.4.1 Combination of 2-DE and MS; 3.4.2 Peptide Mass Fingerprinting; 3.4.3 Peptide Sequencing (PMF); 3.4.4 Shotgun Proteomics; 3.5 Conclusion; Acknowledgments; References; 4: Quantitative Mass Spectrometric Approaches |
4.1 Introduction4.1.1 Gel-Based Quantitative Proteomic Methods; 4.1.2 Shotgun Quantitative Proteomic Methods; 4.1.3 Labeling Methods; 4.1.3.1 Metabolic Incorporation of Stable Isotopes; 4.1.3.2 Enzymatic Incorporation of Stable Isotopes; 4.1.3.3 Chemical Incorporation of Stable Isotopes; 4.2 iTRAQ Analysis of Bacterial Pathogens; 4.2.1 Bacterial Cell Disruption and Protein Extraction; 4.2.2 Determination of Protein Concentration; 4.2.3 Protein Digestion; 4.2.4 Peptide Labeling with iTRAQ Tags; 4.2.5 Protocol for iTRAQ Analysis of Bacterial Proteins; References |
5: BN-PAGE of Microbial Protein Complexes5.1 Introduction; 5.2 Methods for Studying Protein-Protein Interactions; 5.3 Blue Native Polyacrylamide Gel Electophoresis; 5.3.1 Sample Preparation; 5.3.1.1 Non-Denaturing Conditions; 5.3.1.2 Selection of Detergent and Its Optimal Concentration; 5.3.1.3 Membrane and Cytosolic Fraction Separation; 5.3.2 1D BN-PAGE; 5.3.3 2D BN/SDS-PAGE; 5.4 Evaluation of BN-PAGE-Staining, MS, Western Blotting; 5.4.1 Staining; 5.4.1.1 Silver Staining; 5.4.1.2 Fluorescent Staining; 5.4.1.3 Coomassie Staining; 5.4.2 Mass Spectrometry; 5.4.3 Western Blotting |
5.4.4 Other Methods of Visualization |
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Sommario/riassunto |
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Unique coverage of proteomic and glycomic approaches to better distinguish highly dangerous pathogens, as well as using these to explore novel treatment and prevention options. The editors and authors are either part of a specialized European network initiated to develop fast and reliable detection and therapy options, or are associated with the core military research complex of the United States.With its description of the methods, their advantages and limitations, as well as the principle outcomes, this is a must-have resource for all professionals dealing with BSL3 and/or BSL 4 agen |
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2. |
Record Nr. |
UNINA9910411936603321 |
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Autore |
Dixon Brian S. |
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Titolo |
Dewey and Design : A Pragmatist Perspective for Design Research / / by Brian S. Dixon |
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Pubbl/distr/stampa |
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Cham : , : Springer International Publishing : , : Imprint : Springer, , 2020 |
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ISBN |
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Edizione |
[1st ed. 2020.] |
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Descrizione fisica |
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1 online resource (XI, 200 p. 11 illus.) |
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Collana |
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Design Research Foundations, , 2366-4622 |
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Disciplina |
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Soggetti |
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Design |
Pragmatism |
Knowledge, Theory of |
Design, general |
Epistemology |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Preface -- Acknowledgements -- Chapter 1. Design, Pragmatism and Dewey -- Chapter 2. Valuing Experience -- Chapter 3. Knowing in Making -- Chapter 4. Making Things Meaningful -- Chapter 5. Making Things Better -- Chapter 6. Making as Remaking -- Chapter 7. Dewey’s Pragmatism as a Philosophy for Design Research -- References -- Index. . |
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Sommario/riassunto |
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Over the last four decades, John Dewey’s pragmatist philosophy has formed an intellectual core in design research, underpinning Donald Schön’s theory of reflective practice, the experiential perspective in HCI and the democratic commitments of participatory design. Taking these existing connections as a starting point, Brian Dixon explores how deeper alignments may be drawn between Dewey’s insights and contemporary design research’s concern with practice, meaning and collaboration. Chapter by chapter, a fresh intellectual approach is revealed, one which recognises the transformative power of doing, making and knowing as a force for positive change in the world. We see that, for Dewey, experience comes first. It connects us to surrounding world and the society of which we are part; good things can happen |
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and new realities are possible—we just have to work for them. The implications for design research are vast. We are offered a new way of understanding designerly knowledge production, as well as the methodological implications of adopting Deweyan pragmatism in design research. Taken as a whole, Dewey and Design not only draws out the value of Dewey’s work for design research but also, crucially, offers a clear articulation of the value of design itself. |
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