Photonics . Volume IV Biomedical photonics, spectroscopy, and microscopy. : scientific foundations, technology, and applications / / edited by David L. Andrews, School of Chemical Sciences University of East Anglia Norwich, UK ; contributors, Thomas Aabo [and thirty three others]

• Record Nr.: UNINA9910824593303321
• Pubbl/distr/stampa: Hoboken, New Jersey : , : Wiley, , 2015; ©2015
• ISBN: 1-119-01179-5; 1-119-01180-9; 1-119-01402-6
• Descrizione fisica: 1 online resource (602 p.)
• Collana: A Wiley-Science Wise Co-Publication
• Disciplina: 610.28
• Soggetti: Photonics; Spectrum analysis; Microscopy
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: "A Wiley-Science Wise Co-Publication"--Cover.
• Nota di bibliografia: Includes bibliographical references at the end of each chapters and index.
• Nota di contenuto: Photonics; Contents; List of Contributors; Preface; 1 Fluorescence; 1.1 Introduction; 1.2 Spectra; 1.2.1 Background and Theory; 1.2.2 Experimental; 1.2.3 Application Example-Melanin Spectra; 1.3 Quantum Yield; 1.3.1 Theory; 1.3.2 Experimental; 1.3.3 Application Example-ThT Detection of Sheet Structure; 1.4 Lifetime; 1.4.1 Theory; 1.4.2 Experimental; 1.4.3 Application Example-In Vivo Glucose Sensing; 1.5 Quenching; 1.5.1 Theory; 1.5.2 Application-Metal Ion Quenching; 1.6 Anisotropy; 1.6.1 Theory; 1.6.2 Experimental; 1.6.3 Application Example-Nanoparticle Metrology; 1.7 Microscopy; 1.7.1 Systems and Techniques 1.7.2 Application Example-Gold Nanorods in Cells; 1.8 Conclusions; Acknowledgments; 2 Single-Molecule Detection and Spectroscopy; 2.1 Introduction; 2.2 Experimental Setups; 2.2.1 Principles; 2.2.2 Correction of Aberrations; 2.2.3 Polarization Structure at the Focus; 2.2.4 Various Microscopy ; Methods; 2.3 Fluorescence Spectroscopy; 2.3.1 Introduction, Signal-to-Noise Ratio; 2.3.2 Sample Preparation; 2.3.3 Orientation; 2.3.4 Blinking; 2.3.5 Bleaching; 2.3.6 Superresolution; 2.4 Fluorescence Correlation Spectroscopy; 2.4.1 Photon Counting Histograms, Burst Analysis; 2.4.2 Fluorescence Correlation Spectroscopy 2.4.3 Multiparameter Analysis; 2.5 Fluorescence Excitation Spectroscopy; 2.5.1 Zero-Phonon Line and Phonon Wing; 2.5.2 Inhomogeneous Broadening; 2.5.3 Hole-Burning [30]; 2.5.4 Single-Molecule Spectroscopy; 2.5.5 Scope of Low-Temperature Single-Molecule Spectroscopy; 2.6 Other Detection Methods; 2.6.1 General Considerations on Signal, Background, and Noise; 2.6.2 Dark-Field Scattering, Total Internal Reflection; 2.6.3 Absorption, Extinction, Interference-Based Methods; 2.6.4 Pump-Probe and Photothermal Detections; 2.7 Conclusion; Acknowledgments; References 3 Resonance Energy Transfer; 3.1 Introduction; 3.2 History of RET; 3.2.1 The First Experiments; 3.2.2 Early Developments of Theory; 3.2.3 Förster Theory; 3.3 The Photophysics of RET; 3.3.1 Primary Excitation Processes; 3.3.2 Coupling of Electronic Transitions; 3.3.3 Dissipation and Line Broadening; 3.3.4 Förster Equation; 3.3.5 Orientation Dependence; 3.3.6 Polarization Features; 3.3.7 Diffusion Effects; 3.3.8 Long-Range Transfer; 3.3.9 Dexter Transfer; 3.4 Investigative Applications of RET in Molecular Biology; 3.4.1 Spectroscopic Ruler; 3.4.2 Conformational Change; 3.4.3 Intensity-Based Imaging 3.4.4 Lifetime-Based Imaging; 3.4.5 Other Applications; 3.5 The Role of RET in Light-Harvesting Complexes; 3.5.1 Introduction; 3.5.2 Photosynthetic Excitons; Acknowledgments; References; 4 Biophotonics of Photosynthesis; 4.1 Introduction; 4.2 Structure of Pigment-Protein Complexes and Structure-Function Relationships; 4.2.1 Photosystem I (PS I) and Photosystem II (PS II); 4.2.2 PCs of Purple Bacteria; 4.3 Key Concepts in Physics of Pigment-Protein Complexes; 4.3.1 Excitons; 4.3.2 Excitation Energy Transfer; 4.3.3 Homogeneous and In homogeneous Broadening, Zero-Phonon Lines (ZPLs), and Phonon Sidebands (PSBs)
• Sommario/riassunto: Comprehensive and accessible coverage of the whole of modern photonics Emphasizes processes and applications that specifically exploit photon attributes of light Deals with the rapidly advancing area of modern optics